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217 results for “inbreds”
Simulated Ancient Genomic Kinship Dataset: VCF and BAM (1x) Files for Related (including inbred) Pairs
<p>Simulated Ancient Genomic Kinship Dataset: VCF and BAM (1x and 5x) Files for Related (including inbred) Pairs</p><p><strong>Description:</strong></p><p>This dataset comprises simulated pedigrees (VCF files containing 8,677,101 autosomal biallelic and 298,625 X chromosomal SNP positions) generated using Ped-sim (v1.3) and comprising pairs of diverse familial relationship types up to third-degree. The first-degree relationships are parent-offspring and siblings; the second-degree relationships are half-siblings, grandparent-grandchild, and avuncular pairs; and third-degree relationships are first cousins, great-grandparent-great-grandchild, and grand avuncular pairs. For each of these 8 relationship types, our dataset includes 48 pairs of individuals. It also contains unrelated pairs. Additionally, the dataset includes first- and second-degree relatives, with inbreeding (parent-offspring pairs where the parents of the offspring are the first cousins and grandparent-grandchild pairs where the grandchild is the offspring of first cousins). Our simulations encompass all combinations of kinship types regarding sex. The dataset was further enriched by simulating ancient DNA-like sequencing data (5x and 1x BAM files) of Ped-sim simulated individuals using the gargammel tool, employing procedures akin to standard paleogenomic sequencing libraries. Note that the BAM files contain only randomly chosen 200K autosomal SNP positions. Positions can be found in the "200K_positions" file. Details can be found in Aktürk, Mapelli and Güler et al. 2023.</p><p><strong>Data Sources and Generation:</strong></p><p>Founder genotypes for pedigree simulation were created from the Tuscany (TSI) population SNPs within the 1000 Genomes Dataset v3. Notably, the founder genotypes lack background relatedness or runs of homozygosity (ROH).</p><p><strong>Description of File Naming Conventions:</strong></p><p>The naming conventions of the BAM files in this dataset are designed to convey key information regarding the specifics of each file.</p><p><strong>cov1x or cov5x:</strong> This segment denotes the coverage level of the BAM files, indicating whether the sequencing coverage for the individuals in the files is 1x or 5x.</p><p><strong>run_*:</strong> Signifies the particular batch from which the pedigree and individuals are derived. This name segment also applies to VCF files.</p><p><strong>parent-offspring_* or similar identifiers:</strong> Reflects the origin of the individual from the corresponding VCF file. For instance, "parent-offspring_1" corresponds to the individuals present in the "run_*_parent-offspring_1.vcf" file.</p><p><strong>parent-offspring* or similar identifiers:</strong></p><p> Indicates the origin of the individual from the sets within the VCF files. For example, "parent-offspring1" signifies the first set of parent-offspring pedigrees within the VCF file. Note that parent-offspring, grandparent-grandchild, and great-grandparent-great-grandchild and the inbreeding VCFs contain only one set, so this identifier is always 1. This convention can be 1 or 2 for the rest of the pedigrees, as the VCF files contain two sets of related pairs.</p><p><strong>_g*-b*-: </strong>Provides information about the individual's generational level within the VCF. This follows the Ped-sim syntax. For example, for parent-offspring type, "_g1-b1-" indicates the first parent (generation 1) within a specific pedigree, and "_g1-b2-" indicates the second parent (generation 1) while "_g2-b1-" represents the offspring (generation 2).</p><p><strong>Example Naming Structure:</strong></p><p>For instance, the file "cov1x_run1_parent-offspring_1_parent-offspring1_g1-b1-i1.all.hs37d5.cons.90perc.trimBAM.bam" signifies a BAM file with 1x coverage, originating from "run1," containing individuals from the "run_*_parent-offspring_1.vcf" file (first set of parent-offspring pairs) where "_g1-b1-" designates the first parent in the first generation. The latter half of the name "hs37d5.cons.90perc.trimBAM.bam" is the same across all files. </p><p><strong>Note1:</strong> Segments such as <strong>parent-offspring*_g*-b*- </strong>can also be tracked in the naming of the genotype columns in the VCF.</p><p><strong>Note2: </strong>Sexual information within the VCF files is discernible from the genetic data present at X chromosome positions. Individuals carrying two genotypes on the X chromosome are female, while those with a single genotype are male.</p><p><strong>Note3: Some of the individuals from distinct pedigrees may</strong>,<strong> in fact</strong>,<strong> be related due to shared ancestry through common founders. To suit specific research objectives, researchers may need to identify and exclude such relatives if the full dataset is used for kinship estimation.</strong></p><p>For more details about the dataset's generation process, unique characteristics, or any specific inquiries, our team is available for further information. We welcome and encourage inquiries, aiming to provide comprehensive support and additional details that might aid researchers in utilizing this dataset effectively. Please don't hesitate to contact us for any specific information you may need.</p><p>This repository contains only VCFs and cov1x BAM and 200K_positions files. The rest of the files can be found at <strong>10.5281/zenodo.10079625 </strong>and<strong> 10.5281/zenodo.10079685.</strong></p><p> </p>
Simulated Ancient Genomic Kinship Dataset: BAM (5x run1-6) Files for Related (including inbred) Pairs
<p>Simulated Ancient Genomic Kinship Dataset: VCF and BAM (5x (run1-6)) Files for Related (including inbred) Pairs</p> <p><strong>Description:</strong></p> <p>This dataset comprises simulated pedigrees (VCF files containing 8,677,101 autosomal biallelic and 298,625 X chromosomal SNP positions) generated using Ped-sim (v1.3) and comprising pairs of diverse familial relationship types up to third-degree. The first-degree relationships are parent-offspring and siblings; the second-degree relationships are half-siblings, grandparent-grandchild, and avuncular pairs; and third-degree relationships are first cousins, great-grandparent-great-grandchild, and grand avuncular pairs. For each of these 8 relationship types, our dataset includes 48 pairs of individuals. It also contains unrelated pairs. Additionally, the dataset includes first- and second-degree relatives, with inbreeding (parent-offspring pairs where the parents of the offspring are the first cousins and grandparent-grandchild pairs where the grandchild is the offspring of first cousins). Our simulations encompass all combinations of kinship types regarding sex. The dataset was further enriched by simulating ancient DNA-like sequencing data (5x and 1x BAM files) of Ped-sim simulated individuals using the gargammel tool, employing procedures akin to standard paleogenomic sequencing libraries. Note that the BAM files contain only randomly chosen 200K autosomal SNP positions. Positions can be found in the "200K_positions" file. Details can be found in Aktürk, Mapelli and Güler et al. 2023.</p> <p><strong>Data Sources and Generation:</strong></p> <p>Founder genotypes for pedigree simulation were created from the Tuscany (TSI) population SNPs within the 1000 Genomes Dataset v3. Notably, the founder genotypes lack background relatedness or runs of homozygosity (ROH).</p> <p><strong>Description of File Naming Conventions:</strong></p> <p>The naming conventions of the BAM files in this dataset are designed to convey key information regarding the specifics of each file.</p> <p><strong>cov1x or cov5x:</strong> This segment denotes the coverage level of the BAM files, indicating whether the sequencing coverage for the individuals in the files is 1x or 5x.</p> <p><strong>run_*:</strong> Signifies the particular batch from which the pedigree and individuals are derived. This name segment also applies to VCF files.</p> <p><strong>parent-offspring_* or similar identifiers:</strong> Reflects the origin of the individual from the corresponding VCF file. For instance, "parent-offspring_1" corresponds to the individuals present in the "run_*_parent-offspring_1.vcf" file.</p> <p><strong>parent-offspring* or similar identifiers: </strong>Indicates the origin of the individual from the sets within the VCF files. For example, "parent-offspring1" signifies the first set of parent-offspring pedigrees within the VCF file. Note that parent-offspring, grandparent-grandchild, and great-grandparent-great-grandchild and the inbreeding VCFs contain only one set, so this identifier is always 1. This convention can be 1 or 2 for the rest of the pedigrees, as the VCF files contain two sets of related pairs.</p> <p><strong>_g*-b*-: </strong>Provides information about the individual's generational level within the VCF. This follows the Ped-sim syntax. For example, for parent-offspring type, "_g1-b1-" indicates the first parent (generation 1) within a specific pedigree, and "_g1-b2-" indicates the second parent (generation 1) while "_g2-b1-" represents the offspring (generation 2).</p> <p><strong>Example Naming Structure:</strong></p> <p>For instance, the file "cov1x_run1_parent-offspring_1_parent-offspring1_g1-b1-i1.all.hs37d5.cons.90perc.trimBAM.bam" signifies a BAM file with 1x coverage, originating from "run1," containing individuals from the "run_*_parent-offspring_1.vcf" file (first set of parent-offspring pairs) where "_g1-b1-" designates the first parent in the first generation. The latter half of the name "hs37d5.cons.90perc.trimBAM.bam" is the same across all files. </p> <p><strong>Note1:</strong> Segments such as <strong>parent-offspring*_g*-b*- </strong>can also be tracked in the naming of the genotype columns in the VCF.</p> <p><strong>Note2: </strong>Sexual information within the VCF files is discernible from the genetic data present at X chromosome positions. Individuals carrying two genotypes on the X chromosome are female, while those with a single genotype are male.</p> <p><strong>Note3: Some of the individuals from distinct pedigrees may</strong>,<strong> in fact</strong>,<strong> be related due to shared ancestry through common founders. To suit specific research objectives, researchers may need to identify and exclude such relatives if the full dataset is used for kinship estimation.</strong></p> <p>For more details about the dataset's generation process, unique characteristics, or any specific inquiries, our team is available for further information. We welcome and encourage inquiries, aiming to provide comprehensive support and additional details that might aid researchers in utilizing this dataset effectively. Please don't hesitate to contact us for any specific information you may need.</p> <p>This repository contains only cov5x BAM files (run1-6). The rest of the files can be found at <strong>10.5281/zenodo.10079625 </strong>and<strong> 10.5281/zenodo.10070958.</strong></p>
LA1141 × OH8245 inbred backcross (IBC) single nucleotide polymorphism (SNP) markers for genetic studies
<p>The LA1141 × OH8245 157 polymorphic SNP markers from an optimized tomato panel Sim et al., 2012 were used for linkage map construction in the BC<sub>2</sub>S<sub>3</sub> IBC and composite interval mapping QTL analysis. Genetic map position and physical position corresponding to Sl4.0 (Hosmani et al., 2019), and flanking sequences are provided.</p>
Cellular and Humoral Immune Responses after Immunisation with Low Virulent African Swine Fever Virus in the Large White Inbred Babraham Line and Outbred Domestic Pigs
<p>Raw data for manuscript. Contains temperature, clinical scores, qPCR, blood cell numbers and immune responses over time for two groups of pigs immunised with low virulent African swine fever virus and challenged with highly virulent virus. Data for each panel or figure is displayed on a separate worksheet in the file. The readme worksheet contains a brief description of each figure. The majority of data is displayed in an XY table format, with the number of days post immunisation with low virulent virus indicated.</p>
Inbred Strain Variant Database (ISVdb): A repository for probabilistically informed sequence differences among the Collaborative Cross strains and their founders
<p>Data files for the development of a database for storing (and a GUI for retrieving) the imputed variants for 72 Collaborative Cross strains of mice. Files include the inputs for the imputation, as well as the final results. See File_S1_Readme for more details on the included files.</p>
Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato
<p>Wild species are an invaluable source of new traits for crop improvement. Over the years the tomato community bred cultivated lines that carry introgressions from different species of the tomato tribe to facilitate trait discovery and mapping. The next phase in such projects is to find the genes that drive the identified phenotypes. This can be achieved by genotyping a few thousand individuals resulting in fine-mapping that can potentially identify the causative gene. To couple trait discovery and fine mapping we are presenting large, recombination-rich, Backcross Inbred Line (BIL) populations involving an unexplored accession of the wild, green-fruited species Solanum pennellii (LA5240; the Lost Accession) with two modern tomato inbreds: LEA, determinate, and TOP indeterminate. The LEA and TOP BILs are in BC2F6-8 generation and include 1,400 and 500 lines respectively. The BILs were genotyped with ~5,000 SPET markers, showing that in the euchromatic regions there was one recombinant every 17-18 Kb while in the heterochromatin a recombinant every 600-700 Kb (TOP and LEA respectively). To gain perspective on the topography of recombination we compared five independent members of the self-pruning gene family with their respective neighboring genes; based on PCR markers, in all cases we found recombinants. Further mapping analysis of two known morphological mutations that segregated in the BILs (Self-pruning and Hair), showed that the maximal delimited intervals were 73 Kb and 210 Kb respectively and included the known causative genes. The LOST_BILs provide a solid framework to study traits derived from a tolerant wild tomato.</p>
Reciprocal F1 hybrids of two inbred mouse strains reveal parent-of-origin and perinatal diet effects on behavior and expression
<p>Raw data and statistical analyses from an experiment to study parent-of-origin and diet-by-parent-of-origin effects on expression and behavior. </p> <p>In this experiment, female NOD/ShiLtJ x C57Bl/6J and C57Bl/6J x NOD/ShiLtJ mice were exposed in utero to one of four diets. After weaning, their whole-brain gene expression, as well as a set of behaviors that model psychiatric disease, were recorded and analyzed.</p> <p>File_S1_README contains detailed descriptions of all included files.</p>
Pre-existing natural variations of adult neurogenesis and anxiety predict hierarchical social status of inbred male mice.
<p>CVS files and image files of all data presented in the correcponding figures. </p>
From high masked to high realized genetic load in inbred Scandinavian wolves
<p><span>When new mutations arise at functional sites they are more likely to impair than improve fitness. If not removed by purifying selection, such deleterious mutations will generate a genetic load that can have negative fitness effects in small populations and increase the risk of extinction. This is relevant for the highly inbred Scandinavian wolf (<em>Canis</em> <em>lupus</em>) population, founded by only three wolves in the 1980s and suffering from inbreeding depression. We used functional annotation and evolutionary conservation scores to study deleterious variation in a total of 209 genomes from both the Scandinavian and neighboring wolf populations in northern Europe. The masked load (deleterious mutations in heterozygote state) was highest in Russia and Finland with deleterious alleles segregating at lower frequency than neutral variation. Genetic drift in the Scandinavian population led to the loss of ancestral alleles, fixation of deleterious variants and a significant increase in the per-individual realized load (deleterious mutations in homozygote state; an increase by 45% in protein-coding genes) over five generations of inbreeding. Arrival of immigrants gave a temporary genetic rescue effect with ancestral alleles re-entering the population and thereby shifting deleterious alleles from homozygous into heterozygote genotypes. However, in the absence of permanent connectivity to Finnish and Russian populations, inbreeding has then again led to the exposure of deleterious mutations. These observations provide genome-wide insight into the magnitude of genetic load and genetic rescue at the molecular level, and in relation to population history. They emphasize the importance of securing gene flow in the management of endangered populations.<br></span></p>
Structural genomic variation in the inbred Scandinavian wolf population contributes to the realized genetic load but is positively affected by immigration
Open the record for dataset details and reuse information.
Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato
Open the record for dataset details and reuse information.
From high masked to high realized genetic load in inbred Scandinavian wolves
Open the record for dataset details and reuse information.
Simulated Ancient Genomic Kinship Dataset: BAM (5x run7-12) Files for Related (including inbred) Pairs
<p>Simulated Ancient Genomic Kinship Dataset: VCF and BAM (5x (run7-12) Files for Related (including inbred) Pairs</p> <p><strong>Description:</strong></p> <p>This dataset comprises simulated pedigrees (VCF files containing 8,677,101 autosomal biallelic and 298,625 X chromosomal SNP positions) generated using Ped-sim (v1.3) and comprising pairs of diverse familial relationship types up to third-degree. The first-degree relationships are parent-offspring and siblings; the second-degree relationships are half-siblings, grandparent-grandchild, and avuncular pairs; and third-degree relationships are first cousins, great-grandparent-great-grandchild, and grand avuncular pairs. For each of these 8 relationship types, our dataset includes 48 pairs of individuals. It also contains unrelated pairs. Additionally, the dataset includes first- and second-degree relatives, with inbreeding (parent-offspring pairs where the parents of the offspring are the first cousins and grandparent-grandchild pairs where the grandchild is the offspring of first cousins). Our simulations encompass all combinations of kinship types regarding sex. The dataset was further enriched by simulating ancient DNA-like sequencing data (5x and 1x BAM files) of Ped-sim simulated individuals using the gargammel tool, employing procedures akin to standard paleogenomic sequencing libraries. Note that the BAM files contain only randomly chosen 200K autosomal SNP positions. Positions can be found in the "200K_positions" file. Details can be found in Aktürk, Mapelli and Güler et al. 2023.</p> <p><strong>Data Sources and Generation:</strong></p> <p>Founder genotypes for pedigree simulation were created from the Tuscany (TSI) population SNPs within the 1000 Genomes Dataset v3. Notably, the founder genotypes lack background relatedness or runs of homozygosity (ROH).</p> <p><strong>Description of File Naming Conventions:</strong></p> <p>The naming conventions of the BAM files in this dataset are designed to convey key information regarding the specifics of each file.</p> <p><strong>cov1x or cov5x:</strong> This segment denotes the coverage level of the BAM files, indicating whether the sequencing coverage for the individuals in the files is 1x or 5x.</p> <p><strong>run_*:</strong> Signifies the particular batch from which the pedigree and individuals are derived. This name segment also applies to VCF files.</p> <p><strong>parent-offspring_* or similar identifiers:</strong> Reflects the origin of the individual from the corresponding VCF file. For instance, "parent-offspring_1" corresponds to the individuals present in the "run_*_parent-offspring_1.vcf" file.</p> <p><strong>parent-offspring* or similar identifiers: </strong>Indicates the origin of the individual from the sets within the VCF files. For example, "parent-offspring1" signifies the first set of parent-offspring pedigrees within the VCF file. Note that parent-offspring, grandparent-grandchild, and great-grandparent-great-grandchild and the inbreeding VCFs contain only one set, so this identifier is always 1. This convention can be 1 or 2 for the rest of the pedigrees, as the VCF files contain two sets of related pairs.</p> <p><strong>_g*-b*-: </strong>Provides information about the individual's generational level within the VCF. This follows the Ped-sim syntax. For example, for parent-offspring type, "_g1-b1-" indicates the first parent (generation 1) within a specific pedigree, and "_g1-b2-" indicates the second parent (generation 1) while "_g2-b1-" represents the offspring (generation 2).</p> <p><strong>Example Naming Structure:</strong></p> <p>For instance, the file "cov1x_run1_parent-offspring_1_parent-offspring1_g1-b1-i1.all.hs37d5.cons.90perc.trimBAM.bam" signifies a BAM file with 1x coverage, originating from "run1," containing individuals from the "run_*_parent-offspring_1.vcf" file (first set of parent-offspring pairs) where "_g1-b1-" designates the first parent in the first generation. The latter half of the name "hs37d5.cons.90perc.trimBAM.bam" is the same across all files. </p> <p><strong>Note1:</strong> Segments such as <strong>parent-offspring*_g*-b*- </strong>can also be tracked in the naming of the genotype columns in the VCF.</p> <p><strong>Note2: </strong>Sexual information within the VCF files is discernible from the genetic data present at X chromosome positions. Individuals carrying two genotypes on the X chromosome are female, while those with a single genotype are male.</p> <p><strong>Note3: Some of the individuals from distinct pedigrees may</strong>,<strong> in fact</strong>,<strong> be related due to shared ancestry through common founders. To suit specific research objectives, researchers may need to identify and exclude such relatives if the full dataset is used for kinship estimation.</strong></p> <p>For more details about the dataset's generation process, unique characteristics, or any specific inquiries, our team is available for further information. We welcome and encourage inquiries, aiming to provide comprehensive support and additional details that might aid researchers in utilizing this dataset effectively. Please don't hesitate to contact us for any specific information you may need.</p> <p>This repository contains only cov5x BAM files (run7-12). The rest of the files can be found at <strong>10.5281/zenodo.10079685 </strong>and<strong> 10.5281/zenodo.10070958.</strong></p> <p> </p>
SNP markers used for QTL mapping in the inbred lines
<p><span>Young leaves of the 175 inbred lines and their seven parents were collected from seedlings grown in a greenhouse. </span><span>About 200 mg bulk leaf sample from three plants of a line was placed in 2 ml safe-lock </span><span>Eppendorf tube and stored at ‒80 </span><span>˚C for one night prior to crushing using a Mixer Mill (TissueLyser II, Qiagen, Germany). Genomic DNA was extracted using SIGMA DNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer's instruction. DNA concentration and purity of the samples were assessed using a NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The samples were processed and sequenced using tunable genotyping-by-sequencing (tGBS®) method by Data2Bio (Ames, IW, USA). Genomic DNA was digested using two restriction enzymes NSpI (5′-RCATG^Y-3′) and BfuCI/Sau3AI (5′-^GATC-3′) which created 3´and 5´overhangs, respectively. Two single-stranded oligos, one containing a sample-specific internal barcode and the other a universal oligo, were ligated to the complementary 3´ and 5´ overhangs, respectively. </span>All 175 inbred lines' and seven parents' treated DNA was pooled for construction of the tGBS library and sequencing. The raw sequence data were demultiplexed by barcode, which was subsequently removed bioinformatically from each sequence. The barcode-trimmed sequence reads of genotype were further trimmed using the trimming software, Lucy (Chou & Holmes, 2001; Li & Chou 2004) to remove low-quality reads based on Phred quality scores of Q15.</p>
Leveraging interindividual variability in threat conditioning of inbred mice to model trait anxiety
<p><strong>Abstact</strong></p> <p>Trait anxiety is a major risk factor for stress-induced and anxiety disorders in humans. However, animal models accounting for the inter-individual variability in stress vulnerability are largely lacking. Moreover, the pervasive bias of using mostly male animals in preclinical studies poorly reflects the increased prevalence of psychiatric disorders in women. Using the threat imminence continuum theory, we designed and validated an auditory aversive conditioning-based pipeline in both female and male mice. We operationalized trait anxiety by harnessing the naturally occurring variability of defensive freezing responses combined with a model-based clustering strategy. While sustained freezing during prolonged retrieval sessions was identified as an anxiety-endophenotype biomarker in both sexes, females were consistently associated with an increased freezing response. RNA-sequencing of CeA, BLA, ACC and BNST revealed massive differences in phasic and sustained responders’ transcriptomes, correlating with transcriptomic signatures of psychiatric disorders, particularly PTSD. Moreover, we detected significant alterations in the excitation/inhibition balance of principal neurons in the lateral amygdala. These findings provide compelling evidence that trait anxiety in inbred mice can be leveraged to develop translationally relevant preclinical models to investigate mechanisms of stress susceptibility in a sex-specific manner.</p> <p><strong>Remarks</strong></p> <p>This submission contains the analysis code and source data to fully reproduce the behavioural, electrophysiology and RNA-seq analysis described in Kovlyagina et al. </p> <p> - Navigate to the <em>behaviour_ephys</em> directory and run the scripts to reproduce the behavioural and electrophysiology analysis</p> <p>- Navigate to the <em>RNAseq</em> directory and run the script to reproduce the RNA-seq analysis</p> <p> </p>
Data used for 'Parent-offspring inference in inbred populations'
<p>House mouse population founder genotypes used for imputation and simulation</p>
Data from: Sustained positive consequences of genetic rescue of fitness and behavioural traits in inbred populations of Drosophila melanogaster
<p><span>One solution to alleviate the detrimental genetic effects associated with reductions in population size and fragmentation is to introduce immigrants from other populations. While the effects of this genetic rescue on fitness traits are fairly well known, it is less clear to what extent inbreeding depression and subsequent genetic rescue affects behavioural traits. In this study, replicated crosses between inbred lines of <em>Drosophila melanogaster</em> were performed in order to investigate the effects of inbreeding and genetic rescue on egg-to-adult viability and negative geotaxis behaviour - a locomotor response used to measure e.g. the effects of physiological ageing. Transgenerational effects of outcrossing were investigated by examining the fitness consequences in both the F<sub>1</sub> and F<sub>4</sub> generation. The majority of inbred lines showed evidence for inbreeding depression for both egg-to-adult viability and behavioural performance (95% and 66% of lines, respectively), with inbreeding depression being more pronounced for viability compared to locomotor response. Subsequent outcrossing with immigrants led to an alleviation of the negative effects for both viability and geotaxis response resulting in inbred lines being similar to the outbred controls, with beneficial effects persisting from F<sub>1</sub> to F<sub>4</sub>. Overall, the results clearly show that genetic rescue can provide transgenerational rescue of small, inbred populations by rapidly improving population fitness components. Thus, we show that even the negative effects of inbreeding on behaviour, similar to that of neurodegeneration associated with physiological ageing, can be reversed by genetic rescue.</span></p>
Summary data for plots in: Eco-evolutionary extinction and recolonization dynamics reduce genetic load and increase time to extinction in highly inbred populations
<p>Understanding how genetic and ecological effects can interact to shape genetic loads within and across local populations is key to understanding ongoing persistence of systems that should otherwise be susceptible to extinction through mutational meltdown. Classic theory predicts short persistence times for metapopulations comprising small local populations with low connectivity, due to accumulation of deleterious mutations. Yet, some such systems have persisted over evolutionary time, implying the existence of mechanisms that allow metapopulations to avoid mutational meltdown. We first hypothesize a mechanism by which the combination of stochasticity in the numbers and types of mutations arising locally (genetic stochasticity), resulting in local extinction and recolonization through evolving dispersal, facilitates metapopulation persistence. We then test this mechanism using a spatially and genetically explicit individual-based model. We show that genetic stochasticity in highly structured metapopulations can result in local extinctions, which can favour increased dispersal, thus allowing recolonization of empty habitat patches. This causes fluctuations in metapopulation size and transient gene flow, which reduces genetic load and increases metapopulation persistence over evolutionary time. Our suggested mechanism and simulation results provide an explanation for the conundrum presented by the continued persistence of highly structured populations with inbreeding mating systems that occur in diverse taxa.</p>
Data from: Genetic patterns of Magnolia in the Lesser Antilles: Stepwise colonisation leading to highly inbred island 'populations'
<p>Aim: Test for genetic signatures of island biogeographic patterns, using a slowly evolving, recent colonist with a low dispersal capability in an island chain without linear chronosequence; evaluate the multiple-endemic status of the species; and quantify genetic diversity of extant island populations.</p> <p>Location: The Lesser Antilles (Caribbean).</p> <p>Taxon: Magnolia dodecapetala (Magnoliaceae).</p> <p>Methods: Genetic diversity was characterised using Sanger sequencing of 21 individuals amplified for 11 DNA markers, plus microsatellite data of 195 individuals genotyped with 19 simple sequence repeat (SSR) markers. Sanger sequencing data were used to construct a Bayesian phylogenetic hypothesis, while SSR markers were used to run approximate Bayesian computation (ABC) demographic analyses and calculate population statistics.</p> <p>Results: Both types of molecular data support stepwise colonization, decoupled from known island ages. The ABC analyses support a north to south migration while the Sanger sequencing data indicates a mixture of island progression rule and stepping stone dispersal. The SSR data show strong genetic structuring per island and significant inbreeding in all populations except in Saint Lucia. The lowest genetic diversity is found in the population from Saint Vincent. A high amount of genetic linkage occurs in a subpopulation from Dominica.</p> <p>Main conclusions: Biogeographic patterns for the complex geological setting of the Lesser Antilles are uncovered using a slowly evolving study species. All genetic data support treating each island as distinct Management Units for conservation and call for a re-evaluation of the species limits. Inbreeding threatens the survival of island populations and the populations of Saint Vincent and Dominica represent conservation priorities.</p>
Data set for manuscript titled "Morphological, physiological and metabolic responses of diverse barley inbreds to dry down and moderate drought stress"
<p>The primary aim of the study was to understand the genotypic diversity on plant morphology, photosynthetic responses, metabolite shift and their relationship in diverse barley inbreds under dry down (DD) and moderate drought (MD) stress using 23 genetically diverse parental inbreds. The data were collected from over a period of 28 days after the start of stress treatment. The publised data set indcludes the emmeans of all the evaluated characters. Metabolite profiling was done in samples collected from 7 d and 12 d after the start of DD and MD stress.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.