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42 results for “inexpensive”
ThermoCyte: an inexpensive open-source temperature control system for in vitro live cell imaging
<p>Live-cell imaging is a common technique in microscopy to investigate dynamic cellular behaviour and permits the accurate and relevant analysis of a wide range of cellular and tissue parameters, such as motility, cell division, wound healing responses, and calcium (Ca2+) signalling in cell lines, primary cell cultures, and ex vivo preparations. Furthermore, this can take place under many experimental conditions, making live-cell imaging indispensable for biological research. Systems which maintain cells at physiological conditions outside of a CO<sub>2</sub> incubator are often bulky, expensive, and use proprietary components. Here we present an inexpensive, open-source temperature control system for in vitro live cell imaging. Our system 'ThermoCyte', which is constructed from standard electronic components, enables precise tuning, control, and logging of a temperature 'set point' for imaging cells at physiological temperature. We achieved stable thermal dynamics, with reliable temperature cycling and a standard deviation of 0.42°C over 1 hour. Furthermore, the device is modular in nature, and is adaptable to the researcher's specific needs. This represents simple, inexpensive, and reliable tool for laboratories to carry out custom live-cell imaging protocols, on a standard lab bench, at physiological temperature.</p>
Fig. 6 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 6. Predicted number of fruit flies caught relative to trap density in a mango orchard in Haiti during Jan 21 to Mar 3, 2015.
Fig. 2 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 2. Total number of fruit flies caught for each trap model in a mango orchard in Haiti. Bars labeled with the same letter were not significantly different (ANOVA, Fisher LSD α = 0.05).
Fig. 1 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 1. Trap models developed and used for evaluation of mass trapping efficacy for fruit flies in a mango orchard in Haiti. AT1, yellow-bottomed artisanal trap; AT2, clear artisanal trap; and MP, commercial McPhail trap.
Fig. 3 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 3. Average proportion of female and male fruit flies caught per trap model in a mango orchard in Haiti. Bars labeled with identical letters were not significantly different afer comparisons among trap models (ANOVA, Fisher LSD α = 0.05).
Fig. 4 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 4. Total number of fruit flies caught at 24 McPhail per ha (MP/ha) and 36 clear artisanal trap per ha (AT2/ha) in a mango orchard in Haiti. Bars labeled with the same letters were not significantly different (Binomial test for equal proportion, α = 0.05).
Fig. 3 in Characterization of sounds in maize produced by internally feeding insects: investigations to develop inexpensive devices for detection of Prostephanus truncatus (Coleoptera: Bostrichidae) and Sitophilus zeamais (Coleoptera: Curculionidae) in small-scale storage facilities in sub-Saharan Africa
Fig. 3. Effects of distance on detectability of larval sound impulses. Horizontal axis indicates the mean distance between the larval pouch and the sensor; vertical axis indicates the log10-transformed mean rate of impulses detected at that distance. Bars indicate the standard error of mean transformed rate.
Fig. 1 in Characterization of sounds in maize produced by internally feeding insects: investigations to develop inexpensive devices for detection of Prostephanus truncatus (Coleoptera: Bostrichidae) and Sitophilus zeamais (Coleoptera: Curculionidae) in small-scale storage facilities in sub-Saharan Africa
Fig. 1. Spectral profiles of 4 distinctive types of larval sound impulses detected in cracked corn: HaNb, solid line; Ma, dashed line, Ha, dash-dot-dotted line, and La, dotted line. Horizontal axis indicates frequency in kHz and vertical axis indicates relative spectrum amplitude in dB.
Fig. 2 in Characterization of sounds in maize produced by internally feeding insects: investigations to develop inexpensive devices for detection of Prostephanus truncatus (Coleoptera: Bostrichidae) and Sitophilus zeamais (Coleoptera: Curculionidae) in small-scale storage facilities in sub-Saharan Africa
Fig. 2. Oscillogram of sound impulses recorded 10 cm from pouch containing Sitophilus oryzae larvae. Examples of 3 types of larval sound impulse occur during the 1 s period, and one example each of type (Ha, La, and HaNb) is marked above the impulse. Horizontal axis indicates time in seconds and vertical axis indicates relative signal amplitude.
ThermoCyte: an inexpensive open-source temperature control system for in vitro live cell imaging
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An evaluation of inexpensive methods for root image acquisition when using rhizotrons
<p>Dataset presenting the root data analysis performed in the paper:</p> <p><strong>An evaluation of inexpensive methods for root image acquisition when using rhizotrons</strong></p> <p><sup>1</sup>Awaz Mohamed*, <sup>1</sup>Yogan Monnier, <sup>1</sup>Zhun Mao, <sup>2,3</sup>Guillaume Lobet, <sup>4</sup>Jean-Luc Maeght, <sup>1</sup>Merlin Ramel, <sup>1</sup>Alexia Stokes</p> <p>The dataset contains:<br> Images examples_LaboratoryTest.7z: original images from laboratory rhizotron test including examples of images from camera, smartphone and scanners methods before and after image stitching (Test1)<br> Images examples in situ rhizotron.7z: original images of roots system from in situ rhizotron (Test2)<br> Day&night root growth by Camera.7z: original images of root growth monitored by time-lapse camera using in situ rhizotron (Test3).<br> Root_data.CSV.7z: Root data exported from SmartRoot and used for analysis in R<br> Root_Data_Analysis.R.7z: R scripts used for root data analysis</p>
Large and exaggerated sexually selected weapons comprise high proportions of metabolically inexpensive exoskeleton
<p>The cost-minimization hypothesis proposes that positive allometry in sexually selected traits can be explained if the proportional energetic maintenance costs of weapons decrease as traits increase in size. Energetic maintenance costs are the costs of maintaining homeostasis. They are slow, persistent energy sinks that are distinct from ephemeral costs of growth. Because some tissues expend more energy on maintenance than others, energetic maintenance costs can be inferred from proportional tissue composition. For example, soft tissues require more energy for maintenance than exoskeleton, so an arthropod claw that is 50% soft tissue and 50% exoskeleton would have higher energetic maintenance costs than one that is 30% soft tissue and 70% exoskeleton. I tested the cost-minimization hypothesis using proportional tissue composition as a proxy for energetic maintenance costs in snapping shrimp ( Alpheus heterochaelis and Alpheus estuariensis ) and fiddler crabs ( Uca pugilator ). As predicted, larger weapons comprised proportionally less soft tissue mass and more exoskeleton mass than smaller weapons. Furthermore, I extended cost-minimization to explain trait exaggeration: individuals might exaggerate traits by investing more mass in exoskeleton. As predicted, exoskeleton mass proportional to weapon mass increased as exaggeration increased. These results support and extend the cost-minimization hypothesis to explain positive allometry and weapon exaggeration.</p>
Fig. 5 in Inexpensive artisanal traps for mass trapping fruit flies (Diptera: Tephritidae) in Haiti
Fig. 5. Numbers of fruit flies caught by trap density in a mango orchard in Haiti.
Data from: An inexpensive and open-source method to study large terrestrial animal diet and behavior using time-lapse video and GPS
1. The behavior of free-ranging animals is difficult to study, especially on the large spatial and temporal scales relevant to long-lived large species. Animal-borne video and environmental data collection systems (AVEDs) record behavior and other data in real time as animals conduct daily activities. However, few studies have combined systematically collected, long term AVED foraging data with environmental and movement data to test hypotheses on animal foraging. Additionally, AVEDs are often either prohibitively expensive, or require extensive fabrication and programming knowledge. 2. The video and coordinate animal-mounted system (VACAMS) is an animal-mounted data collection system based on a modified GoPro® action camera platform that records short, first "person" perspective videos of animal behavior on an automated time-lapse schedule. As most videos are georeferenced, researchers can return to the locations of specific behaviors and collect accurate, fine-grained data on non-woody vegetation and other habitat characteristics that may influence animal behavior. Moreover, VACAMS are inexpensive and easy to use. 3. This study describes VACAMS preliminary data on cattle foraging and a hypothesis exploring free-ranging cattle browsing habits throughout the rainy season in the tropical dry forest of Sonora, Mexico. I generated a database of vegetation types consumed by cows each month (Annual, Woody, and Leaf litter) and compared actual vegetation type frequencies to a priori assumptions based on seasonal patterns of forage availability. During the monsoons, when palatable vegetation was abundant, frequencies of annual and woody perennial vegetation in cattle diets did not differ from month to month. When the rains ceased and palatable vegetation became scarce, cows switched to leaf litter, dead annual vegetation, twigs, and dried leguminous fruits. 4. Open source software and commercially available hardware make VACAMS financially attainable for many researchers, land managers, students, and other user groups. VACAMS could be used on a range of domestic and semi-domestic free-ranging animals, particularly in dense forests where conventional observations are impossible. With improvements to GPS battery life and durability, the weakest points of the system, VACAMS could also potentially apply to studies of other large terrestrial animals.
Cell-free Synthesis of Proteins with Selectively 13C-Labelled Methyl Groups from Inexpensive Precursors- Dataset
<p>NMR Spectra relating to the publication:<br> <br> Cell-free Synthesis of Proteins with Selectively 13C-Labelled Methyl Groups from Inexpensive Precursors.</p> <p>Spectra are labelled as such in the folder name and in the description.</p>
Accurate, Rapid and Inexpensive MRI Protocol for Breast Cancer Screening
ClinicalTrials.gov study NCT04877912. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Data from: An inexpensive and open-source method to study large terrestrial animal diet and behavior using time-lapse video and GPS
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Large and exaggerated sexually selected weapons comprise high proportions of metabolically inexpensive exoskeleton
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Rapid and Inexpensive Whole-Genome Sequencing of SARS-CoV2 using 1200 bp Tiled Amplicons and Oxford Nanopore Rapid Barcoding
<p>Description of 1200bp amplicon primer sets and .bed and .tsv files for SARS-CoV-2 assembly using the ARTIC bioinformatics pipeline.</p>
Can Simple and Inexpensive Techniques Enhance Patient Comfort
ClinicalTrials.gov study NCT00747084. IPD Sharing: Not stated. Countries: 1. Publications: 1.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.