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Fig. 2 in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy

Fig. 2 Box plots displaying correlations between cell size (of motile cells; A, B) or side length (of empty thecate cells; C) in selected strains. Colours correspond to the ribotype of each strain (blue: ribotype I; magenta: ribotype II; orange: ribotype III). Statistically significant clusters are indicated with letters a, b and c and were calculated with Tukey's Honest Significant Difference (HSD) test (p-values <0.05). Box plots depict percentile values from 25–75% (box), median (bar inside the box), standard deviation (whiskers) and outliers (dots)

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◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy

◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV

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◂Fig. 4 A molecular tree of 51 systematically representative Peridiniaceae, including all 28 accessions assignable to P. volzii. Maximum Likelihood tree (–ln = 22,017.62), as inferred from a rRNA nucleotide alignment (1,129 parsimony-informative sites) and with strain number information. Numbers on branches are ML bootstrap (above) and Bayesian support values (below) for the clusters (asterisks indicate maximal support values, values under 50 and 0.90, respectively, are not shown). Clades are indicated (abbreviations: HET, Heterocapsaceae; PPE, Protoperidiniaceae) in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy

◂Fig. 4 A molecular tree of 51 systematically representative Peridiniaceae, including all 28 accessions assignable to P. volzii. Maximum Likelihood tree (–ln = 22,017.62), as inferred from a rRNA nucleotide alignment (1,129 parsimony-informative sites) and with strain number information. Numbers on branches are ML bootstrap (above) and Bayesian support values (below) for the clusters (asterisks indicate maximal support values, values under 50 and 0.90, respectively, are not shown). Clades are indicated (abbreviations: HET, Heterocapsaceae; PPE, Protoperidiniaceae)

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Supplementary material 4 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Table 3. Data partition characteristics, summary statistics and models of the phylogenetic analyses : Explanation note: For each data partition (ITS, ETS, trnT-trnL-trnF, rpoB-trnC, MatK) and concatenated dataset (plastid and nuclear), the following are given: number of sequences, number of characters, number of parsimony informative (PI) characters, % parsimony informative characters, maximum parsimony tree length (L), number of most parsimonious trees, consistency index excluding uninformative characters (CI) and retention index (RI). Also given are the models used in the Bayesian analyses as determined using the Akaike information criterion (AIC) in jModeltest.

opencc-zeroNov 2018View details →
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Supplementary material 3 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Table 2. Poa and outgroup samples used in the phylogenetic analyses : Explanation note: Poa and outgroup samples used in the phylogenetic analyses with subgeneric classification (subtribe for outgroups), voucher information and GenBank Accession numbers for each of the five DNA regions (ITS, ETS, trnT-trnL-trnF, matK, rpoB-trnC).

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Supplementary material 1 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Table 1. Chromosome numbers in taxa of Poasubg.Secundae : Explanation note: Chromosome numbers in taxa of Poasubg.Secundae, with RJS' subspecies determinations and original determinations as published or found on herbarium sheets, literature reference, number of counts, voucher collection, country and state or province abbreviation and herbarium where deposited, if known. CI = Carnegie Institution. These numbers show the hexaploid nature of the species complex and the wide and differing ranges of chromosome numbers in each of the P.secunda subspecies.

opencc-zeroNov 2018View details →
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Supplementary material 2 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

David D. Keck's annotations of taxa here included in Poasecunda : Explanation note: The following taxa recognised by Keck, but included by us in P.secunda s.l., are given in the specimen annotation lists: P.ampla, P.canbyi, P.gracillima, P.incurva, P.juncifolia, P.nevadensis, P.sandbergii and P.scabrella. His lists focused on western Continental United States species but included some mainly non-arctic Alaskan, Canadian and Mexican (Baja California) records and some records of eastern United States species. Copies of the original typed lists are stored in the reprint files in the Grass Lab in the Department of Botany, Smithsonian Institution. Optical character recognition (OCR) was performed on the present selection to allow the lists to be searchable to a large degree. Keck's annotations are considered to be sound and to represent hundreds of historical collections widely distributed in herbaria as vouchers for P.secunda infraspecies. We treat P.ampla, P.juncifolia and P.nevadensis as varieties of P.secundasubsp.juncifolia (vars. ampla, juncifolia and nevadensis, respectively) in our revised classification. The remaining taxa are treated as varieties of P.secundasubsp.secunda , as follows: var. gracillima (P.gracillima), var. scabrella (P.scabrella) and var. secunda (P.canbyi, P.incurva, and P.sandbergii).

opencc-zeroNov 2018View details →
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FIGURE 7. Gelidocalamus stellatus var. wugongshanensis. A in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 7. Gelidocalamus stellatus var. wugongshanensis. A. habit; B. new shoot; C–F. culm leaf sheath; G. hollow internode, longitudinal section; H. node and buds; I. foliage leaf, the abaxially basal; J. foliage leaf sheath. (Scale bars = 0.5 m [A]; 1 cm [B–F]; 0.5 cm [G–J])

opennotspecifiedJun 2018View details →
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FIGURE 2 in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 2. Specimens of the taxa of Gelidocalamus analyzed in this study. A. Gelidocalamus monophyllus (Zhang & Liu 20161024001); B. G. stellatus var. stellatus (Yang & Zhang JGS003); C. G. stellatus var. mangshanensis (Zhang & Liu RJD0102); D. G. stellatus var. wugongshanensis (Yang & Zhang JGS107).

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FIGURE 1 in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 1. SEM images of the abaxial leaf epidermis. A. Gelidocalamus monophyllus (Jiuyi Mountain, Hunan, China). B. G. stellatus var. stellatus (Jinggang Mountain, Jiangxi, China). C. G. stellatus var. wugongshanensis (Wugong Mountain, Jiangxi, China). D. G.stellatus var. mangshanensis (Mangshan, Hunan, China).

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FIGURE 6. Gelidocalamus stellatus var. mangshanensis. A in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 6. Gelidocalamus stellatus var. mangshanensis. A. habit; B. fully mature plant; C. new shoot; D. branching and leaves; E. node and branches; F. young culm, transverse section; G. node and internodes; H–K. culm leaves; L. the apical of new shoot; M. foliage leaf sheath. (Scale bars = 1 m [A]; 10 cm [B–D, L]; 0.5 cm [E–K, M])

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FIGURE 4 in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 4. Geographical distribution of Gelidocalamus monophyllus (rhombus) and Gelidocalamus stellatus (pentacle). Solid pentacles, empty pentacles and gray pentacles represent three varieties, i.e. G. stellatus var. stellatus, G. stellatus var. wugongshanensis and G. stellatus var. mangshanensis, respectively.

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FIGURE 5. Gelidocalamus stellatus var. stellatus. A in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 5. Gelidocalamus stellatus var. stellatus. A. habit; B. mature plant; C. new shoot; D–F. culm and branches; G–K. culm leaves; L. twig; M. foliage leaf. (Scale bars = 1 m [A]; 5 cm [B–E, G, H, L, M]; 0.5 cm [F, I–K])

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FIGURE 3. Gelidocalamus monophyllus. A in Re-evaluation of the taxonomy of Gelidocalamus stellatus (Poaceae: Bambusoideae) and its infraspecific taxa from southern China

FIGURE 3. Gelidocalamus monophyllus. A. habit; B. mature culm; C–E. new shoot and rhizomes; F. branches and foliage leaf; G–M. young culm and node (G and H), culm leaf sheath when young (H and I), old culm and node (J), culm leaf sheath when old (K and L), and branches (M). (Scale bars = 0.5 m [A–B]; 5 cm [C–F]; 1 cm [G–M])

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◂Fig. 3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar= 10 µm. U A= 15 kV in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy

◂Fig. 3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar= 10 µm. U A= 15 kV

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Figure 4 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Figure 4 Poasecunda infraspecies illustrations (reproduced from Hitchcock 1935): AP.s.var.ampla panicle and floret BP.s.var.juncifolia panicle and floret CP.s.var.nevadensis panicle and floret DP.s.var.gracillima habit, panicle and floret EP.s.var.scabrella habit, panicles, spikelet and floret F, GP.s.var.secunda habit, panicle and floret variations A, B, C = subsp. juncifoliaD, E, F, G = subsp. secunda. Scale bar: 5 mm for florets, 10 mm for spikelet, 5 cm for habits and panicles (10 cm for E habit and panicles).

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Figure 2 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Figure 2 Distribution of Poasecunda subspecies in North America: subsp. secunda (yellow) ; subsp. juncifolia (blue).

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Figure 1 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Figure 1 Poasecunda habit and panicles: AP.s.subsp.secundavar.secunda (Soreng 9359) BP.s.subsp.juncifoliavar.ampla (Soreng 9358) C Panicles of subsp. secundavar.secunda (left) and juncifolia var. ampla (right) (Photos. RJS, Deschutes River near Madras, Jefferson Co., Oregon).

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Figure 3 from: Soreng RJ, Gillespie LJ (2018) Poa secunda J. Presl (Poaceae): a modern summary of infraspecific taxonomy, chromosome numbers, related species and infrageneric placement based on DNA. PhytoKeys 110: 101-121. https://doi.org/10.3897/phytokeys.110.27750

Figure 3 Bayesian 50% majority rule consensus trees of Poa based on plastid (trnT-trnL-trnF, rpoB-trnC, MatK) data (left) and nrDNA ITS and ETS data (right). Bayesian posterior probabilities are shown above branches, maximum parsimony bootstrap values below branches. Outgroups are not shown. Major clades are indicated by colour and capital letters. Taxa shown in blue belong to P.subg.Secundae; those in grey are other taxa of putative hybrid origin that belong to different major clades in plastid and nrDNA trees.

opencc-by-4.0Nov 2018View details →

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