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ShareScore release 0.9.0
Dataset results
47 results for “intracellular signals”
p53 alters intracellular Ca2+ signaling through regulation of TRPM4
<p>Altered expression of transient receptor potential channel melastatin 4 (TRPM4) contributes to several malignancies, including cardiac conduction diseases, immune diseases, and cancer. Yet the underlying mechanisms of TRPM4 expression changes remain elusive.In this study, we report that loss of tumor suppressor protein p53 or p63γ function or mutation of a putative p53 response element in the TRPM4 promoter region increase TRPM4 promoter activity in the colorectal cancer cell line HCT 116. In cells that lack p53 expression, we observed increased TRPM4 mRNA and protein levels and TRPM4-mediated Na<sup>+</sup> currents. This phenotype can be reversed by transient overexpression of p53. In the prostate cancer cell line LNCaP, which expresses p53 endogenously, p53 overexpression decreases TRPM4-mediated currents. As in other cancer cells, CRISPRcas9 mediated knockout of TRPM4 in p53 deficient HCT 116 cells results in increased store-operated Ca<sup>2+</sup>entry. The effect of the TRPM4 knockout is mimicked by p53 mediated suppression of TRPM4 in the parental cell line expressing TRPM4. In addition, a TRPM4 knockout-mediated shift in cell cycle is abolished upon loss of p53.Taken together, these findings indicate that p53 represses TRPM4 expression, thereby altering cellular Ca<sup>2+</sup> signaling and that TRPM4 adds to cell cycle shift dependent on p53 signaling.</p>
Magnetic manipulation of intracellular signals
<p>In the context of the MAGNEURON project we have developed magnetic tools to manipulate intracellular proteins involved in intracellular signaling processes. We focused on the manipulation of the subdomain DHPH of ITSN1 in order to control the activity of CDC42.</p> <p>To control the activity of CDC42 we produced a fusion protein consisting in the DHPH domain of ITSN1, the mCherry fluorescent protein and a nanobody against GFP (aGFPnb).</p> <p>The manipulations were done either using a magnetic tip or magnetic micropillars done with soft magnetic material (Nickel Iron alloy). Nanoparticles were first injected in the cytoplasm of the cell. After a delay of at least ten minutes, the survival of the cell was controlled and the manipulation could start.<br> We monitored the fluorescence of the reporter (IRFP-NWASP) and the formation of protrusion/filopodia as markers of a biological response to the manipulation.</p> <p>We observed protrusion formation as well as NWASP activity when attracting the nanoparticles, but control experiments (Manipulation of particles without ITSN1-mcherry-aGFPnb) also showed increase of fluorescence of the NWASP reporter at the point of attraction of the particles.</p> <p>In addition, if compared to optogenetic manipulation of the similar ITSN1-DHPH domain, the formation of protrusion was very limited. We concluded that magnetic manipulation of intracellular signals was not efficient to hijack signaling pathways.</p> <p>We publish here the raw data of some experiments that were produced in the lab.</p> <p>Each experiment has a README file describing the parameter of the recording and what can be observe on the video</p>
Impaired intracellular Ca2+ signaling contributes to age-related cerebral small vessel disease in Col4a1 mutant mice
<p>Humans and mice with mutations in <em>COL4A1</em> and <em>COL4A2</em> manifest hallmarks of cerebral small vessel disease (cSVD). Mice with a missense mutation in <em>Col4a1</em> at amino acid 1344 (<em>Col4a1<sup>+/G1344D</sup></em>) exhibit age-dependent intracerebral hemorrhage (ICH) and brain lesions. Here we report that this pathology was associated with the loss of myogenic vasoconstriction, an intrinsic vascular response essential for the autoregulation of cerebral blood flow. Electrophysiological analyses showed that the loss of myogenic constriction resulted from blunted pressure-induced smooth muscle cell (SMC) membrane depolarization. Further, we found that dysregulation of membrane potential was associated with impaired Ca<sup>2+</sup>-dependent activation of large-conductance Ca<sup>2+</sup>-activated K<sup>+</sup> (BK) and transient receptor potential melastatin 4 (TRPM4) cation channels linked to disruptions in sarcoplasmic reticulum (SR) Ca<sup>2+</sup> signaling. Treating <em>Col4a1<sup>+/G1344D</sup></em> mice with 4-phenylbutyrate, a compound that promotes the trafficking of misfolded proteins and alleviates SR stress, restored SR Ca<sup>2+</sup> signaling and BK and TRPM4 channel activity, prevented loss of myogenic tone, and reduced ICH. We conclude that alterations in SR Ca<sup>2+</sup> handling that impair membrane potential regulating ion channel activity result in dysregulation of SMC membrane potential and loss of myogenic tone contributing to age-related cSVD in <em>Col4a1<sup>+/G1344D</sup></em> mice.</p>
Data from: Characterization of binding kinetics and intracellular signaling of new psychoactive substances targeting cannabinoid receptor using transition-based reweighting method
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Impaired intracellular Ca2+ signaling contributes to age-related cerebral small vessel disease in Col4a1 mutant mice
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Effects of Free Fatty Acids and 3-hydroxybutyrate on Protein, Glucose, Lipid Metabolism and Intracellular Signals.
ClinicalTrials.gov study NCT01752348. IPD Sharing: Not stated. Countries: 1. Publications: 1.
A Study of Intracellular Signaling in Muscle and Fat Cells During Ketosis
ClinicalTrials.gov study NCT02157155. IPD Sharing: Not stated. Countries: 1. Publications: 14.
TPL-2;ERK1/2 signaling promotes host resistance against intracellular bacterial infection by negative regulation of type I interferon production [Set 1]
GEO Series GSE47672. Mus musculus. 26 samples. Type: Expression profiling by array.
Salmonella activation of STAT3 signaling by SarA effector promotes intracellular replication and production of IL-10
GEO Series GSE104354. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Artificial neural networks enable genome-scale simulations of intracellular signaling
GEO Series GSE202515. Homo sapiens. 190 samples. Type: Expression profiling by high throughput sequencing.
T cell-intrinsic MyD88 signaling in cognate immune response to intracellular parasite infection: crucial role for IL-18R
GEO Series GSE57738. Mus musculus. 4 samples. Type: Expression profiling by array.
Host succinate is a signal for activation of Salmonella virulence during intracellular infection
GEO Series GSE139208. Salmonella enterica subsp. enterica serovar Typhimurium; Mus musculus. 72 samples. Type: Expression profiling by high throughput sequencing.
The Unique Role of Intracellular Perinuclear β1-Adrenergic Receptors in Defining Signaling Compartmentation and Pathological Cardiac Remodeling.
GEO Series GSE305499. Rattus norvegicus. 24 samples. Type: Expression profiling by high throughput sequencing.
Intracellular accumulation of IFN-λ4 contributes to protection from liver cirrhosis by inducing ER stress and enhancing IRF1 signaling
GEO Series GSE145038. Homo sapiens. 54 samples. Type: Expression profiling by high throughput sequencing.
Inositol pyrophosphate InsP8 acts as intracellular phosphate signal in Arabidopsis
GEO Series GSE126927. Arabidopsis thaliana. 6 samples. Type: Expression profiling by high throughput sequencing.
TPL-2;ERK1/2 signaling promotes host resistance against intracellular bacterial infection by negative regulation of type I interferon production [Set 2]
GEO Series GSE47673. Mus musculus. 132 samples. Type: Expression profiling by array.
Discovery and functional assessment of a novel adipocyte population driven by intracellular Wnt/β-catenin signaling in mammals
GEO Series GSE164747. Mus musculus. 14 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics
GEO Series GSE162461. Homo sapiens. 16 samples. Type: Expression profiling by high throughput sequencing; Other.
Intracellular C3 regulates the immune response to infection via NF-κB signaling
GEO Series GSE301708. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
Pro-survival lipid metabolism activates intracellular complement signaling to induce inflammasome-mediated tumor metastasis
GEO Series GSE169443. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.