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9 results for “isothermal amplification”
Figure 1 in Diagnostic accuracy of loop-mediated isothermal amplification (LAMP) for screening patients with imported malaria in a non-endemic setting
Figure 1. Study flow chart.
Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'
<p>Here we present images of loop-mediated isothermal amplification (LAMP) and optimized Cap-iLAMP (capture and improved loop-mediated isothermal amplification). Cap-iLAMP combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. The sensitivity is 97% and the specificity is 99%.</p>
Data from: Sex determination in the wild: a field application of loop-mediated isothermal amplification successfully determines sex across three raptor species
PCR-based methods are the most common technique for sex determination of birds. Although these methods are fast, easy and accurate, they still require special facilities that preclude their application outdoors. Consequently, there is a time lag between sampling and obtaining results that impedes researchers to take decisions in situ and in real time considering individuals' sex. We present an outdoor technique for sex determination of birds based on the amplification of the duplicated sex-chromosome-specific gene Chromo-Helicase-DNA binding protein using a loop-mediated isothermal amplification (LAMP). We tested our method on Griffon Vulture (Gyps fulvus), Egyptian Vulture (Neophron percnopterus) and Black Kite (Milvus migrans) (family Accipitridae). We introduce the first fieldwork procedure for sex determination of animals in the wild, successfully applied to raptor species of three different subfamilies using the same specific LAMP primers. This molecular technique can be deployed directly in sampling areas because it only needs a voltage inverter to adapt a thermo-block to a car lighter and results can be obtained by the unaided eye based on colour change within the reaction tubes. Primers and reagents are prepared in advance to facilitate their storage at room temperature. We provide detailed guidelines how to implement this procedure, which is simpler (no electrophoresis required), cheaper and faster (results in c. 90 min) than PCR-based laboratory methods. Our successful cross-species application across three different raptor subfamilies posits our set of markers as a promising tool for molecular sexing of other raptor families and our field protocol extensible to all bird species.
Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'
Open the record for dataset details and reuse information.
Data from: Sex determination in the wild: a field application of loop-mediated isothermal amplification successfully determines sex across three raptor species
Open the record for dataset details and reuse information.
Designing loop-mediated isothermal amplification (LAMP) and assimilating probes
<p>On March 28, 2020, IPANEMA organized the first Scientific online lecture (SOL1) for the project’s partners. dr Ryo Kubota, a molecular biologist from <a href="http://diagenetix.com/">Diagenetix</a> - a member of the IPANEMA consortium, was presenting his scientific expertise in designing Loop-mediated isothermal AMPlification (LAMP) and Assimilating Probes.</p>
Video data describing bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells
<p>Video data of loop-mediated isothermal nucleic acid amplification (LAMP) and bioluminescent assay in real time (BART) in microfluidicaly produced droplets. The videos are the data used to generate still images for figures in the source publication "Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells".</p> <p>Details of the videos acquisition parameters are as follows:</p> <ul> <li>Video 1: LAMP-BART droplets in tubing - no DNA template control (Figure 2a) (assay duration 600s, frames 1 per 10s, 60 frames, final</li> <li>Video 2 frames per second) Video 2: LAMP-BART droplets in tubing - positive DNA template control (Figure 2b) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)</li> <li>Video 3: LAMP-BART parked droplets (Figure 3) (assay duration 600s, frames 1 per 10s, 60 frames, final video 3.3 frames per second)</li> <li>Video 4: LAMP-BART DNA gradient in tubing (Figure 4) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)</li> <li>Video 5: LAMP-BART eDIBs (Figure 5) (assay duration 1570s, frames 1 per 10s, 157 frames, final video 5.2 frames per second)</li> <li>Video 6: One to four core LAMP-BART eDIBs (Figure 6) (assay duration 1200s, frames 1 per 10s, 120 frames, final video 5 frames per second)</li> </ul> <p>When referencing please use:</p> <p>Hardinge, P., Baxani, D. K., McCloy, T., Murray, J. A. H. & Castell, O. K. Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells. <em>Sci Rep</em> <strong>10</strong>, 21886 (2020).</p>
Supplementary materials - A finger-actuated sample-dosing capillary-driven microfluidic device for loop-mediated isothermal amplification
<p>These videos illustrate the filling process occured in the finger-actuated capillary-driven microfluidc. Video S1 shows the filling process when using the food dye as the sample. Video S2 shows the filling process occured in the finger-actuated microfluidic devices with pre-stored LAMP master mix when the sample input was water for No template control (N1) and cDNA of N-gene SARS-CoV-2 (P2).</p>
Use of an Isothermal Linear Amplification Method with Small Samples on DNA Microarrays
GEO Series GSE2252. Mus musculus; unidentified; Homo sapiens. 15 samples. Type: Expression profiling by array.
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