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9 results for “light and scanning electron microscopy”
◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy
◂Fig. 1 Morphology of thecate and coccoid cells, with labelled thecal plates. a–c, i, m Light microscopy, d–h, k–l scanning electron microscopy. a Ventral view of strain GeoM*788; b dorsal view of strain GeoM*793; c apical view of strain GeoK*044; d ventral view of strain GeoK*037; e dorsal view of strain GeoM*788; f apical view of strain GeoK*024, with the dehiscence of epithecal opening indicated by a blue line; g antapical view of strain GeoK*044; h leftlateral view of strain GeoM*866; i motile cell of strain GeoK*037; k–m coccoid cells showing variability in shape and size of strains k GeoM*866, l GeoM*793 and m GeoK*024. Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, Sa: anterior sulcal plate, Sd: right sulcal plate, Sp: posterior sulcal plate. Ss: left sulcal plate. Scale bar: 10 µm. UA: 15 kV
Data from: Crustacean photoreceptor damage and recovery: Applying a novel scanning electronic microscopy protocol in artificial light at night studies
<p>As sources of artificial light at night (ALAN) expand worldwide, research on their impacts has also increased. Most of these studies, including those in coastal habitats, have focused on behavioral and ecological responses to ALAN, overlooking impacts on the photoreceptor, the basic functional structure of animals to absorb light. Examining structural changes in the photoreceptor is essential to understand the mechanisms by which ALAN may be impacting species, particularly those adapted to different light backgrounds. This study examined the photoreceptor (rhabdom) of two sandy beach crustaceans exhibiting different light tolerances at night: the amphipod <em>Orchestoidea tuberculata</em> and the isopod <em>Tylos spinulosus</em>. We developed a novel protocol to measure these species' photoreceptor areas and quantify the damage caused by ALAN using histological sections and scanning electron microscopy (SEM). Our results showed that in the isopod, a species naturally adapted to lower light intensities at night than the amphipod, the rhabdom surface was 20-times larger, and presented a tapetum, an adaptive feature found in species living in low light conditions. This confirmed that this species is potentially more sensitive to ALAN than the amphipod. Consistently, a brief period of exposure to ALAN (1 h, 20 lux) caused 3-6 times more damage in the isopod' rhabdom. In fact, ALAN caused structural damage in the isopod' but not in the amphipod' rhabdom, a damage that did not show signs of recovery from ALAN after 1 and 24 h. Thus, the damage caused by ALAN on an organism's photoreceptors is likely to be more severe and persistent in species naturally adapted to lower light levels at night. Installation of permanent ALAN sources nearby the burrowing area of these light sensitive species may have differential effects on their activity and interactions at night. ALAN may also become a new selection pressure on these species, a concern with wide implications given the ubiquity among animals of the photoreceptor structure and its response to light.</p>
Data from: Crustacean photoreceptor damage and recovery: Applying a novel scanning electronic microscopy protocol in artificial light at night studies
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FIGURE 5–10. Amblyomma oblongoguttatum larva. 5 in Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy
FIGURE 5–10. Amblyomma oblongoguttatum larva. 5. Gnathosoma, dorsal view (30 µm); 6 Gnathosoma, ventral view (30 µm); 7. Detail of tibiotarsus and hypostome (30 µm); 8. Detail of scutum (30 µm); 9. Coxae I–III (20 µm); 10.Tarsus I, dorsal view (30 µm).
FIGURE 2. Amblyomma oblongoguttatum larva. Tarsus I in Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy
FIGURE 2. Amblyomma oblongoguttatum larva. Tarsus I dorsal views. Abbreviations: d—dorsal; a— antiaxial; p—paraxial; la—lateral anterior; lp—lateral posterior.
FIGURE 1 in Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy
FIGURE 1. Amblyomma oblongoguttatum larva. Gnathosoma dorsal (right) and ventral (left) views. Abbreviations: d—dorsal; v—ventral; a—antiaxial; p—paraxial; t—terminal; F—femur; G—genu; Tt—tibiotarsus.
FIGURE 4 in Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy
FIGURE 4. Amblyomma oblongoguttatum larva. Segmentation model of the idiosoma. Segments are indicated by Roman numbers (III–VI e VIII–XIV) and delimitated by dashed lines (----); series are indicated by Arabic numbers and delimitated by dotted lines (........). Integumentary structures are illustrated.
FIGURE 3. Amblyomma oblongoguttatum larva. Tarsus I in Redescription of the larva of Amblyomma oblongoguttatum Koch, 1844 (Acari: Ixodidae) by light and scanning electron microscopy
FIGURE 3. Amblyomma oblongoguttatum larva. Tarsus I ventral views. Abbreviations: v—ventral; a— antiaxial; p—paraxial; la—lateral anterior; lp—lateral posterior.
◂Fig. 3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar= 10 µm. U A= 15 kV in Morphological and molecular variability of Peridinium volzii Lemmerm. (Peridiniaceae, Dinophyceae) and its relevance for infraspecific taxonomy
◂Fig. 3 Historically described phenotypical variations and yet undiscovered deviations in the plate pattern of P. volzii. b–c, f, i, l–m Light microscopy, a, d–e, g–h, k scanning electron microscopy. a–f Newly identified deviations a–b plate 4′′ pentagonal in strains a GeoM*793; b GeoM*788; c plate 2a split (strain GeoK*024); d plates 2′′ and 3′′ fused (strain GeoM*866); e plates 1′′′ and 1′′′′ fused (strain GeoM*788); f plates 1a and 3′ fused (strain GeoM*788). g–m Historic infraspecific taxa; g P. guestrowiense forma lineatum (strain GeoM*866); h P. guestrowiense forma compressum (strain GeoM*866); i P. guestrowiense subvar. originale (strain GeoK*024); k P. volzii var. cinctiforme (strain GeoM*793); l P. volzii var. simplex (strain GeoM*789); m P. volzii forma complexum (strain GeoM*793). Abbreviations: n′: apical plate, n′′: precingular plate, n′′′: postcingular plate, n′′′′: antapical plate, na: anterior intercalary plate, nC: cingular plate, split or fused plates are indicated by asterisks. Scale bar= 10 µm. U A= 15 kV
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