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64 results for “mechanotransduction”
Role of TRP channels in dinoflagellate mechanotransduction
<p>This repository contains several files associated with the publication:</p> <p><strong>Transcriptome:</strong> <em>Lingulodinium polyedra </em>transcriptome assembled from 100 bp paired-end Illumina RNA sequences available at http://www.ebi.ac.uk/ (run accession SRR1184657) using the assembly and annotation pipeline “MakeMyTranscriptome” (https://github.com/bluegenes/MakeMyTranscriptome), which leverages the Trinity <em>de novo</em> assembler (v2.1.1; Grabherr 2011). The transcriptome contains 183,451 transcripts, with an N50 of 1161, and 66.5% GC (guanine-cytosine) content. Annotation-based assessment using BUSCO showed that this transcriptome contained 62% of eukaryotic genes predicted to be present in all eukaryotic assemblies. Open reading frames (ORFs) predicted using transDecoder are also provided as a second file (ends with .pep).</p> <p><strong>TRP-like polypeptide sequences: </strong>Transient Potential Receptor (TRP)-like polypeptide sequences identified in transcriptomic data from the dinoflagellate <em>Lingulodinium polyedra</em>.</p> <p><strong>Video: </strong>Video of luminescence from the dinoflagellate <em>Lingulodinium polyedra</em> in response to capsaicin treatment. Samples consisted of 1 ml volumes of <em>L. polyedra</em> culture containing approximately 7000 cells, kept in two separate glass vials. The injection of 0.1 ml volumes of control solution (left vial) and capsaicin (30 µM final concentration, right vial) at a rate of 1ml min<sup>-1</sup> caused some light response due to stimulation of cells by the fluid addition. However, following fluid addition the capsaicin treatment clearly stimulated luminescence in the <em>L. polyedra</em> cells, seen as individual sources of light, while the cells in the control treatment produced essentially no light.</p>
Data for: The energetics of rapid mechanotransduction
<p>Cells throughout the human body detect mechanical forces. While it is known that the rapid (millisecond) detection of mechanical forces is mediated by force-gated ion channels, a detailed quantitative understanding of cells as sensors of mechanical energy is still lacking. Here, we combine atomic force microscopy with patch-clamp electrophysiology to determine the physical limits of cells expressing the force-gated ion channels Piezo1, Piezo2, TREK1, and TRAAK. We find that, depending on the ion channel expressed, cells can function either as proportional or non-linear transducers of mechanical energy, detect mechanical energies as little as ~100 fJ, and with a resolution of up to ~1 fJ. These specific energetic values depend on cell size, channel density, and cytoskeletal architecture. We also make the surprising discovery that cells can transduce forces either nearly instantaneously (<1 ms), or with substantial time delay (~10 ms). Using a chimeric experimental approach and simulations we show how such delays can emerge from channel-intrinsic properties and the slow diffusion of tension in the membrane. Overall, our experiments reveal the capabilities and limits of cellular mechanosensing and provide insights into molecular mechanisms that different cell types may employ to specialize for their distinct physiological roles.</p>
Asymmetric mechanotransduction by hair cells of the zebrafish lateral line (Part 1/2)
<p>In the lateral line system, water motion is detected by neuromast organs, fundamental units that are arrayed on a fish's surface. Each neuromast contains hair cells, specialized mechanoreceptors that convert mechanical stimuli, in the form of water movement, into electrical signals. The orientation of hair cells' mechanosensitive structures ensures that the opening of mechanically-gated channels is maximal when deflected in a single direction. In each neuromast organ, hair cells have two opposing orientations, enabling bi-directional detection of water movement. Interestingly, Tmc2b and Tmc2a proteins, which constitute the mechanotransduction channels in neuromasts, distribute asymmetrically so that Tmc2a is expressed in hair cells of only one orientation. Here, using both<em> in vivo</em> recording of extracellular potentials and calcium imaging of neuromasts, we demonstrate that hair cells of one orientation have larger mechanosensitive responses. The associated afferent neuron processes that innervate neuromast hair cells faithfully preserve this functional difference. Moreover, Emx2, a transcription factor required for the formation of hair cells with opposing orientations, is necessary to establish this functional asymmetry within neuromasts. Remarkably, loss of Tmc2a does not impact hair cell orientation but abolishes the functional asymmetry as measured by recording extracellular potentials and calcium imaging. Overall, our work indicates that oppositely oriented hair cells within a neuromast employ different proteins to alter mechanotransduction to sense the direction of water motion.</p>
Asymmetric mechanotransduction by hair cells of the zebrafish lateral line (Part 2/2)
<p>In the lateral line system, water motion is detected by neuromast organs, fundamental units that are arrayed on a fish's surface. Each neuromast contains hair cells, specialized mechanoreceptors that convert mechanical stimuli, in the form of water movement, into electrical signals. The orientation of hair cells' mechanosensitive structures ensures that the opening of mechanically-gated channels is maximal when deflected in a single direction. In each neuromast organ, hair cells have two opposing orientations, enabling bi-directional detection of water movement. Interestingly, Tmc2b and Tmc2a proteins, which constitute the mechanotransduction channels in neuromasts, distribute asymmetrically so that Tmc2a is expressed in hair cells of only one orientation. Here, using both<em> in vivo</em> recording of extracellular potentials and calcium imaging of neuromasts, we demonstrate that hair cells of one orientation have larger mechanosensitive responses. The associated afferent neuron processes that innervate neuromast hair cells faithfully preserve this functional difference. Moreover, Emx2, a transcription factor required for the formation of hair cells with opposing orientations, is necessary to establish this functional asymmetry within neuromasts. Remarkably, loss of Tmc2a does not impact hair cell orientation but abolishes the functional asymmetry as measured by recording extracellular potentials and calcium imaging. Overall, our work indicates that oppositely oriented hair cells within a neuromast employ different proteins to alter mechanotransduction to sense the direction of water motion.</p>
Data from: Complexes of vertebrate TMC1/2 and CIB2/3 proteins form hair-cell mechanotransduction cation channels
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Asymmetric mechanotransduction by hair cells of the zebrafish lateral line (Part 2/2)
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Data for: The energetics of rapid mechanotransduction
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Asymmetric mechanotransduction by hair cells of the zebrafish lateral line (Part 1/2)
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Data from: LHFPL5 is a key element to the gating spring of cochlear hair cells: comparison of mechanotransduction channel activation in presence or absence of LHFPL5
<p>During auditory transduction, sound-evoked vibrations of the hair cell stereociliary bundles open mechanotransducer (MET) ion channels via tip links extending from one stereocilium to its neighbor. How tension in the tip link is delivered to the channel is not fully understood. The MET channel comprises a pore-forming subunit, transmembrane channel-like protein (TMC1 or TMC2), aided by several accessory proteins, including LHFPL5 (lipoma HMGIC fusion partner-like 5). We investigated the role of LHFPL5 in transduction by comparing MET channel activation in outer hair cells of <em>Lhfpl5-/- </em>knockout mice with those in <em>Lhfpl5+/-</em> heterozygotes. The 10-90 percent working range of transduction in <em>Tmc1+/+; Lhfpl5+/-</em> was 52 nm, from which the single-channel gating force, Z was evaluated as 0.34 pN. However, in <em>Tmc1+/+; Lhfpl5‑/- </em>mice,<em> </em>the<em> </em>working range increased to 123 nm and Z more than halved to 0.13 pN, indicating reduced sensitivity. Tip link tension is thought to activate the channel via a gating spring, whose stiffness is inferred from the stiffness change on tip link destruction. The gating stiffness was ~40 percent of the total bundle stiffness in wild-type but was virtually abolished in <em>Lhfpl5-/-,</em> implicating LHFPL5 as a principal component of the gating spring. The mutation <em>Tmc1 </em>p.D569N reduced the LHFPL5 immunolabeling in the stereocilia and like <em>Lhfpl5-/-</em> doubled the MET working range but other deafness mutations had no effect on the dynamic range. We conclude that tip-link tension is transmitted to the channel primarily via LHFPL5; residual activation without LHFPL5 may occur by direct interaction between PCDH15 and TMC1.</p>
Ca2+ imaging data for: A rigidity transition of MEC-2/Stomatin condensates controls neuronal mechanotransduction during touch sensing
<p>Calcium imaging data from Sanfeliu et al, NCB, 2023.</p> <p>Project contains fluorescence data to characterize the activity of the touch receptor neurons (TRNs) - which are mechanically activated upon touch – in the model organism <em>Caenorhabditis elegans</em>. The set of data includes wild-type animals as a reference and two different mutants to understand their role during touch sensation: MEC-2(R385H) and UNC-89 knock-out.</p> <p> </p>
Data from: LHFPL5 is a key element to the gating spring of cochlear hair cells: comparison of mechanotransduction channel activation in presence or absence of LHFPL5
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RNA-seq and CUT&Tag-seq datasets for MDA-MB-231 and MCF-10A cells from: Sp1 mechanotransduction regulates breast cancer cell invasion in engineered viscoelastic extracellular matrices
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Data from: Mechanotransduction current is essential for stability of the transducing stereocilia in mammalian auditory hair cells
Mechanotransducer channels at the tips of sensory stereocilia of inner ear hair cells are gated by the tension of 'tip links' interconnecting stereocilia. To ensure maximal sensitivity, tip links are tensioned at rest, resulting in a continuous influx of Ca2+ into the cell. Here we show that this constitutive Ca2+ influx, usually considered as potentially deleterious for hair cells, is in fact essential for stereocilia stability. In the auditory hair cells of young postnatal mice and rats, a reduction in mechanotransducer current, via pharmacological channel blockers or disruption of tip links, leads to stereocilia shape changes and shortening. These effects occur only in stereocilia that harbor mechanotransducer channels, recover upon blocker washout or tip link regeneration, and can be replicated by manipulations of extracellular Ca2+ or intracellular Ca2+ buffering. Thus, our data provide the first experimental evidence for the dynamic control of stereocilia morphology by the mechanotransduction current.
Data from: Mechanotransduction current is essential for stability of the transducing stereocilia in mammalian auditory hair cells
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Disrupting Mechanotransduction Decreases Fibrosis and Contracture in Porcine Split Thickness Skin Grafting
GEO Series GSE190849. Sus scrofa. 5 samples. Type: Expression profiling by high throughput sequencing.
Piezo1-mediated Mechanotransduction Shapes ILC2 Translational Activity, Functions, and Lung Pathogenicity
GEO Series GSE278085. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Hic-5 drives epithelial mechanotransduction promoting a feed-forward cycle of bronchoconstriction.
GEO Series GSE310981. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
PRR14 mediates mechanotransduction and regulates myofiber identity via MEF2C in skeletal muscle
GEO Series GSE243333. Mus musculus. 6 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Wnt5a-YAP signaling axis mediates mechanotransduction in cardiac myocytes and contributes to the transition to heart failure
GEO Series GSE200667. Mus musculus; Rattus norvegicus. 32 samples. Type: Expression profiling by high throughput sequencing.
RNA-seq analysis of equine tenocytes exposed to siRNA targeting scleraxis (Scx) to identify novel targets of Scx-mediated mechanotransduction
GEO Series GSE110567. Equus caballus. 6 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.