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5 results for “membrane feeding”
A feed-forward pathway drives LRRK2 kinase membrane recruitment and apparent activation
<p>Activating mutations in the Leucine Rich Repeat Kinase 2 (LRRK2) cause Parkinson's disease and activated LRRK2 phosphorylates a subset of Rab GTPases. Moreover, Golgi-associated Rab29 can recruit LRRK2 to the surface of the Golgi and activate it there for both auto- and Rab substrate phosphorylation. Here we define the precise Rab29 binding region of the LRRK2 Armadillo domain between residues 360-450 and show that this site, termed "Site #1", can also bind additional LRRK2 substrates, Rab8A and Rab10. Moreover, we identify a distinct, N-terminal, higher affinity interaction interface between LRRK2 phosphorylated Rab8 and Rab10 termed "Site #2", that can retain LRRK2 on membranes in cells to catalyze multiple, subsequent phosphorylation events. Kinase inhibitor washout experiments and mutation analysis demonstrate that rapid recovery of kinase activity in cells depends on the ability of LRRK2 to associate with phosphorylated Rab reaction products. Reconstitution of purified LRRK2 recruitment onto planar lipid bilayers decorated with Rab10 protein demonstrates cooperative association of only active LRRK2 with phospho-Rab10-containing membrane surfaces. These experiments reveal a feed-forward pathway that provides spatial control and apparent membrane activation of LRRK2 kinase activity.</p>
Figure 3–Figure Supplement 4 of the paper 'A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation'
<p>Raw immunoblotting and quantitation data for Figure 3–Figure Supplement 4 of the paper "A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Activation" (Edmundo G. Vides, Ayan Adhikar, Claire Y. Chiang, Pawel Lis, Elena Purlyte, Charles Limouse, Justin L. Shumate, Elena Spínola-Lasso, Herschel S. Dhekne, Dario R. Alessi, and Suzanne R. Pfeffer)</p> <p><strong>File descriptions:</strong></p> <p><strong>Figure 3–Figure Supplement 4A</strong><br> <strong>Fig.3.S4A_700(tRab10;tLRRK2)_High.tif </strong>- Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4A_700(tRab10;tLRRK2)_Low.tif</strong> - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; low contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4A_800(pRab10;pLRRK2)_High.tif</strong> - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4A_800(pRab10;pLRRK2)_Low.tif</strong> - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4A_HA.tif </strong>- ChemiDoc Scan (ECL) showing HA blot</p> <p><strong>Figure 3–Figure Supplement 4B</strong></p> <p><strong>Fig.3.S4B_700(tRab10;tLRRK2)_High.tif </strong>- Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4B_700(tRab10;tLRRK2)_Low.tif</strong> - Licor scan (channel 700) showing total Rab10 and total LRRK2 blots; low contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4B_800(pRab10;pLRRK2)_High.tif</strong> - Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4B_800(pRab10;pLRRK2)_Low.tif </strong>- Licor scan (channel 800) showing Rab10 pT73 and LRRK2 pS935 blots; high contrast<br> Panel 1 - top left membrane<br> Panel 2 - top right membrane<br> Panel 3 - bottom left membrane<br> Panel 4 - bottom right membrane</p> <p><strong>Fig.3.S4B_HA.tif </strong>- ChemiDoc Scan (ECL) showing HA blot</p> <p><strong>Fig.3.S4_immunoblotting_numerical_data.xlsx</strong> - Excel sheet containing raw quatitation values from Licor scans as well as calculated pRab10/Rab10 ratios (values used in Figure 3C and 3D)</p>
A feed-forward pathway drives LRRK2 kinase membrane recruitment and apparent activation
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A novel positive feed-back loop mediated by the membrane-associated transcription factor NTL3 is important for heat stress tolerance in rice
GEO Series GSE122021. Oryza sativa. 12 samples. Type: Expression profiling by high throughput sequencing.
The Effect of β-Carotene, Vitamin D3 and Zinc on Hyaline Membrane Disease and Feeding Intolerance in Premature Neonates
ClinicalTrials.gov study NCT03366584. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
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