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135 results for “microsatellite marker”
SBC LTER: Reef: Microsatellite markers for the giant kelp, Macrocystis pyrifera
These data were published as a technical note: Alberto, F., A. C. Whitmer, N. C. Coelho, M. Zippay, E. Varela-Alvarez, P. T. Raimondi, D. C. Reed and E. A. Serrão. 2009. Microsatellite markers for the giant kelp Macroystis pyrifera. Conservation Genetics. 10:1915-1917 doi: 10.1007/s10592-009-9853-9 Abstract: We report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. Markers were obtained by screening a genomic library enriched for microsatellite motifs. Of the 37 primer pairs defined, 16 amplified clean polymorphic microsatellites and are described. These loci identified a number of alleles ranging from three to forty (mean= 16.5, and gene diversity ranging from 0.469 to 0.930 (mean= 0.774). The isolation and characterization of these highly polymorphic markers will greatly benefit much needed studies on the molecular ecology of this important macroalga. These data report the isolation and characterization of 16 microsatellite loci to study the population genetics of the giant kelp, Macrocystis pyrifera. 180 samples of blade tissue were collected at Carpinteria Reef, Santa Barbara Channel, CA (USA) in July 2006. Sequence data are available from GenBank. These data were published as a Technical Note in the journal Conservation Genetics in 2009.
SBC LTER: Reef: Geospatial structure of microsatellite markers for the giant kelp, Macrocystis pyrifera, Santa Barbara CA, 2009
Data package includes data and R code for the examination of geospatial genetic structure (SGS) and simulation of inbreeding in giant kelp (Macrocystis pyrifera) from the Santa Barbara Channel, California. Data are reported for microsatellite markers from individual giant kelp plants from Carpinteria Reef, Mohawk Reef and Goleta Bay, collected during September 2009. Relative X and Y coordinates (meters) were recorded for each specimen sampled. R code consists of two scripts, a) to calculate mean number of alleles per locus, and observed and expected heterozygosity, and b) to simulate self-fertilization and sibling/cousin relationships. These data were presented in: Johansson, Mattias L, Raimondi, Peter T, Reed, Daniel C, Coelho, Nelson C, Serrão, Ester A, Alberto, Filipe A. In press. Looking into the black box: simulating the role of self-fertilization and mortality in the genetic structure of Macrocystis pyrifera. Molecular Ecology, 22:4842–4854. These data are also available from: Johansson ML, Raimondi PT, Reed DC, Coelho NC, Serrão EA, Alberto FA (2013) Data from: Looking into the black box: simulating the role of self-fertilization and mortality in the genetic structure of Macrocystis pyrifera. Dryad Digital Repository. doi:10.5061/dryad.s1b07.
Kelp Metapopulations: Macrocystis pyrifera microsatellite marker biogeography study
Dataset contains microsatellite genotypes specific for Macrocystis pyrifera (giant kelp). Table 1 describes seven loci from blades collected from 62 sites from Alaska, USA, to Baja California, Mexico, and Table 2 blades collected at 38 sites (subpopulations) in central California (Monterey Bay). Each row is the multilocus genotype for a single specimen (individual). These data were described in <ulink url="http://dx.doi.org/10.1111/mec.13371">Johansson ML, Alberto F, Reed DC, Raimondi PT, Coelho NC, Young MA, Drake PT, Edwards CA, Cavanaugh K, Assis J, Ladah LB, Bell TW, Coyer JA, Siegel DA, Serrão EA (2015) Seascape drivers of Macrocystis pyrifera population genetic structure in the northeast Pacific. Molecular Ecology. 24, 4866–4885.</ulink>
Fig. 1 in Molecular Characterization Of Lates Niloticus (Perciformes, Latidae) Populations From Three Nigerian Waterbodies Using Random Amplified Polymorphic Dna And Microsatellite Markers
Fig. 1. Map showing the sample locations of L. niloticus (Linnaeus, 1758). Population 1 — Kainji lake, Population 2 — River Benue, Makurdi and Population 3 — Ikere-Gorge reservoir, Iseyin, Oyo state.
Figure 3 in Genome-wide characterization of microsatellites and development of polymorphic markers shared between two weevils of Eucryptorrhynchus (Coleoptera: Curculionidae)
Figure 3. Genetic structure of Eucryptorrhynchus brandti (a) and E. scrobiculatus (b) populations based on 14 microsatellite markers inferred using the software STRUCTURE. Each bin indicates an individual. Different colors show the identified clusters. The best number of clusters (K) is 3. Abbreviations: BJHD—Haidian District, Beijing; NXZW—Zhongwei, Ningxia; SDTA—Tai'an, Shandong; SXYL—Yangling, Shaanxi.
Figure 2 in Genome-wide characterization of microsatellites and development of polymorphic markers shared between two weevils of Eucryptorrhynchus (Coleoptera: Curculionidae)
Figure 2. Frequency distribution of microsatellites among different motifs in the Eucryptorrhynchus brandti and E. scrobiculatus. The "others" category represents summed motifs with counts below 100.
Figure 1 in Genome-wide characterization of microsatellites and development of polymorphic markers shared between two weevils of Eucryptorrhynchus (Coleoptera: Curculionidae)
Figure 1. Collection sites for specimens of Eucryptorrhynchus brandti (red) and E. scrobiculatus (green). Abbreviations: BJHD— Haidian District, Beijing (116.22°E, 40.04°N); NXZW—Zhongwei, Ningxia (105.12°E, 37.50°N); SDTA—Tai'an, Shandong (116.72°E, 36.27°N); SXYL—Yangling, Shaanxi (108.07°E, 34.26°N).
Monitoring Plasmodium falciparum and Plasmodium vivax using microsatellite markers indicates limited changes in population structure after substantial transmission decline in Papua New Guinea
Monitoring the genetic structure of pathogen populations may be an economical and sensitive approach to quantify the impact of control on transmission dynamics, highlighting the need for a better understanding of changes in population genetic parameters as transmission declines. Here we describe the first population genetic analysis of the major human malaria parasites, <i>Plasmodium falciparum</i> (Pf) and <i>Plasmodium vivax</i> (Pv) populations following nationwide distribution of long-lasting insecticide treated nets (LLIN) in Papua New Guinea (PNG). Parasite isolates from pre- (2005-6) and post-LLIN (2010-2014) were genotyped using microsatellite markers. Despite parasite prevalence declining substantially (East Sepik: Pf=54.9-8.5%, Pv=35.7-5.6%, Madang: Pf=38.0-9.0%, Pv: 31.8-19.7%), genetically diverse and intermixing parasite populations remained. Pf diversity declined modestly post-LLIN relative to pre-LLIN (East Sepik: Rs = 7.1-6.4, He = 0.77-0.71; Madang: Rs= 8.2-6.1, He = 0.79-0.71). Unexpectedly, population structure present in pre-LLIN populations was lost post-LLIN, suggesting that more frequent human movement between provinces may have contributed to higher gene flow. Pv prevalence initially declined but increased again in one province, yet diversity remained high throughout the study period (East Sepik: Rs=11.4-9.3, He=0.83-0.80; Madang: Rs=12.2-14.5, He=0.85-0.88). Although genetic differentiation values increased between provinces over time, no significant population structure was observed at any time point. For both species, a decline in multiple infections and increasing clonal transmission and significant multilocus linkage disequilibrium (mLD) post-LLIN was a positive indicator of impact on the parasite population using microsatellite markers. These parameters may be useful adjuncts to traditional epidemiological tools in the early stages of transmission reduction.
Development of twenty-four microsatellite markers for Afrotropical Ornithodoros ticks
<p><strong>Background: </strong>Soft ticks of the genus <em>Ornithodoros</em> are responsible for the maintenance and transmission of the <em>African swine fever </em>(ASF)<em> virus</em> in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of <em>Borrelia</em> species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical <em>Ornithodoros </em>ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study was to design microsatellite markers that could be used for ticks of the <em>Ornithodoros moubata</em> complex, particularly <em>Ornithodoros phacochoerus</em>, to assess population structure and tick movements in ASF endemic areas.</p> <p><strong>Methods: </strong>One hundred and fifty-one markers were designed using the <em>O. moubata </em>and <em>O. porcinus</em> genomes after elimination of repeated sequences in the genomes. All designed markers were tested on <em>O. phacochoerus </em>and <em>O. porcinus </em>DNA to select the best markers.</p> <p><strong>Results:</strong> Twenty-four microsatellite markers were genotyped on two populations of <em>O. phacochoerus</em> and on few individuals from four other <em>Ornithodoros</em> species. Nineteen markers were selected to be as robust as possible for population genetic studies on <em>O. phacochoerus</em>.</p> <p><strong>Conclusions:</strong> The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of Afrotropical <em>Ornithodoros</em>. This dataset contains the genotyping results obtained for all twenty-four markers tested.</p>
Panmixia and active colonisation of the invasive palm Trachycarpus fortunei (Arecaceae) in Southern Switzerland and Northern Italy as inferred by microsatellites and SNP markers
<p>Dataset for the paper named "Panmixia and active colonisation of the invasive palm Trachycarpus fortunei (Arecaceae) in Southern Switzerland and Northern Italy as inferred by microsatellites and SNP markers"</p> <p>GBS analysis:</p> <p>- variants.vcf.gz : compressed non filtered VCF file with 208 samples and 73685 markers on 36195 loci</p> <p>- variants.filt.vcf.gz: Filtered Variant call file (compressed) - Samples with > 50% missing genotypes, and variants with genotype calls in less than 80% of samples are removed; variants with maf < 1% are removed -207 samples and 31312 markers on 19301 loci - 1 samples removed 6CL</p> <p>Microsatellites:</p> <p>TFT.fortunei_Microsatellites_FSTATFINAL_Pop.dat</p> <p>Samples file</p> <p>-Trachycarpus_Samples_sheet.xlsx : list of samples used (lab extractions) </p> <p> </p> <p> </p>
T a b l e 4 in Molecular Characterization Of Lates Niloticus (Perciformes, Latidae) Populations From Three Nigerian Waterbodies Using Random Amplified Polymorphic Dna And Microsatellite Markers
T a b l e 4. Microsatellites results
Characterization and microsatellite marker development for Geosmithia obscura, a common bark and ambrosia beetle associate
<p class="MsoNormal"><strong><span>Background. </span></strong><span>S</span><span>ymbioses between <em>Geosmithia</em> fungi and </span><span>wood-boring and bark beetles</span><span> seldom result in disease induction within the plant host. Yet exceptions exist such as <em>Geosmithia</em> <em>morbida</em>, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts and <em>Geosmithia</em> sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of<em> G. obscura </em>were recovered from black walnut trees in eastern Tennessee and<em> </em>at least one isolate induced cankers following artificial inoculation. Due to the putative pathogenicity and lack of recovery of <em>G. obscura </em>from natural lesions, a molecular diagnostic screening tool was developed using microsatellite markers mined from the <em>G. obscura </em>genome.</span></p> <p class="MsoNormal"><strong><span>Results. </span></strong><span>A total of 3,256 candidate microsatellite markers were identified (2236, 789, 137 di-, tri-, and tetra- motifs were identified, respectively), with 2011, 703, 101 di-, tri-, and tetra- motifs containing markers with primers. From these, 75 microsatellite markers were randomly selected, screened, and optimized, resulting in 28 polymorphic markers that yielded single, consistently recovered bands which were used in downstream analyses. Five of these microsatellite markers were found to be specific to <em>G. obscura </em>and did not cross-amplify into other, closely related species. Although the remaining tested markers could be useful, they cross-amplified within different <em>Geosmithia</em> species, making them not reliable for <em>G. obscura </em>detection.</span></p> <p class="MsoNormal"><strong><span>Conclusion.</span></strong><span> Five novel microsatellite markers (GOBS9, GOBS10, GOBS41, GOBS43, GOBS50) were developed based on <em>G. obscura</em> genome. These species-specific microsatellite markers are available as a tool for use in molecular diagnostics and can assist future surveillance studies.</span></p>
Nuclear genetic diversity and structure of Anastrepha ludens wild populations evidenced by microsatellite markers
<p class="MsoNormal"><span>The Mexican fruit fly, <em>Anastrepha ludens </em>is an important pest that causes widespread damage to a number of fruit crops in Mexico. The Sterile Insect Technique (SIT) is commonly used for its control. However, the existence of natural barriers can give rise to a population structure in neutral loci and possibly behavioral or adaptive traits that interfere with SIT. For this reason, it is important to understand the genetic diversity and structure of<em> A. ludens </em>populations and to better understand the evolutionary ecology and population processes in view of possible expansions and possible host shifts due to climate change. We genotyped nine nuclear DNA (nDNA) microsatellite loci among fruit fly populations collected from five biogeographic areas within Mexico, Mexican Plateau, the Northeastern Coastal Plain, the Pacific Coast, the Gulf Coast of Mexico, the Soconusco and a laboratory strain. The nuclear genetic diversity was moderate (from <em>H</em>e = 0.34 to <em>H</em>e = 0.39) within the wild mexfly population. We found that populations were clustered in three genetic groups (<em>K</em>=3). The diversity and genetic structure of <em>A. ludens</em> are produced by environmental and geological conditions as well as local conditions like anthropogenic perturbation which would produce population expansion and the existence of possible predators that would affect the population density. Gene flow showed recent migration among populations. The laboratory strain showed less diversity than the wild samples. Large values of current and ancestral population size suggest high resistance to climatic changes, probably due to biological attributes, such as its polyphagous, multivoltine and high dispersal characteristics. In particular ecosystem fragmentation and perturbation as well as the existence of new plant hosts all of which would probably increase the abundance of flies.</span></p>
De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea)
<p class="MsoBodyText">Approaches using microsatellite markers are considered the gold standard for modern population-genetic studies. However, though they have found application in research into various platyhelminth taxa, they remained substantially underutilized in the study of monogeneans. In the present study, a newly-developed set of 24 microsatellite markers was used to investigate the genetic diversity of the generalist monogenean species <i>Dactylogyrus vistulae</i>. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among <i>D. vistulae </i>individuals in the southern Balkans (mean N<sub>A </sub>per locus = 3.917), suggesting that generalist <i>D. vistulae </i>expanded from the south to the north in the Balkans and later putatively into central Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of <i>D. vistulae </i>is associated with the diversification of their cyprinoid hosts. In addition, the partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier to gene flow, even for generalist parasite species.</p>
Fig.1 in Testing The Microsatellites-Pcr Markers For Genetic Diversity Research Of Alien Ponto-Caspian Amphipod Pontogammarus Robustoides G. O. Sars, 1894
Fig.1. Localities of sampling sities in the Latvian reservoirs.
Data from: Novel microsatellite markers for epiphytic bromeliad Tillandsia recurvata L., in an urban landscape in South-eastern Brazil
<p><span>The authors present seven novel microsatellite markers for </span><em>Tillandsia recurvata</em> L. The genome assemble sequences of <em>T. recurvata</em> were obtained from NCBI (7.2Gb) (Sayers <em>et al</em>. 2022). The BioProject Accession and accession numbers are PRJNA701548 and SRX10089449, respectively. The microsatellite identification software Krait (0.5.2) (Du <em>et al</em>. 2018) was used to detect suitable microsatellites, both genome-wide and in the noncoding regions of <em>T. recurvata</em>. The single sequence repeats (SSRs) were refined to a minimum number of seven repeats of di-, tri-, or tetra-nucleotide repeat motifs. These sequences were further limited to SSRs of more than 100bp in length and low GC content (<50%). Krait (0.5.2) (Du <em>et al</em>. 2018) was also used to design the primers for the selected SSR sequences, in conjunction with the integrated Primer3 software. The criteria for primer selection included: a primer length of 18-26bp, an optimal melting temperature (Tm) of 54-59°C and GC content of <50%. These designed primers were single-plexed and amplified using the following PCR cycle: initial denaturation (95°C for 3 min), 34 cycles of 95°C for 30s, annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD: 56°C, 19286TD: 53°C, 5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min and a final extension of 72°C for 5 mins. The authors make this information available to other researchers, to continue the investigation of epiphyte genetics.</p>
Data from: genetic resources of macroalgae: development of an efficient method using microsatellite markers in non-model organisms
<p><span>Red and brown seaweeds are species with high ecological and economic importance. Here we report the feasibility of cost-effective molecular marker development in 6 species from different clades. Microsatellites markers of two brown seaweed species <em>Alaria esculenta</em>, <em>Pylaiella littoralis</em>, and of four red seaweed species <em>Calliblepharis jubata</em>, <em>Gracilaria gracilis</em>, <em>Gracilaria dura </em>and <em>Palmaria palmata</em> were identified and characterized using genomic sequences of Double-Digest Restriction site Associated DNA (ddRAD). A total of 64,623,186 reads were generated from two runs of multiplexed Illumina Miseq sequencing for which 30,636 reads containing microsatellites and 15,443 microsatellite loci with primers pairs were found. Five hundred seventy-six primers pairs were selected for amplification trials and levels of polymorphism. From the 338 that gave a positive amplification, 142 primers pairs were polymorphic. For genetic analyses two or three populations per species from 13 different geographic locations were used. A total of 28 usable polymorphic markers for <em>A. esculenta</em>, 18 for <em>P. littoralis</em>, 11 for <em>C. jubata</em>, 14 for <em>G. gracilis</em>, 21 for <em>G. dura </em>and 13 for <em>P. palmata </em>were developed. The overall number of alleles per locus ranged from 2 to 22. These 105 new microsatellite markers will be useful for further studies of population genetics, breeding programs and conservation genetics of these species. Compared with traditional approaches, our study yielded thousands of microsatellite loci in a short tim</span><span>e with affordable costs in six different species. This study based on ddRAD-sequencing for the development of microsatellite markers provides preliminary data u</span><span>sing a few individuals from two distinct populations on the genetic structure and reproduction mode of a non-model species as shown </span>with the detection of clonality for the two red algae, <em>C. jubata </em>and <em>G. dura</em> and the detection of highly genetically divergent populations corresponding probably to different cryptic species under the name of<em> P. littoralis</em>.</p>
Genotype data of Philippine native pigs, Duroc, Landrace, Large White and Berkshire, using 20 ISAG-FAO recommended microsatellite markers
<p>Microsatellite genotyping is a cost-effective method for the genetic diversity analysis of under-studied populations, such as the Philippine native pigs. We genotyped <em>n</em> = 196 pigs representing 7 Philippine native pig populations (<em>n </em>= 20 to 27 for each population) and 4 commercial transboundary breeds (<em>n</em> = 9 to 11 for each population). Twenty microsatellite markers, recommended by the International Society of Animal Genetics (ISAG)-FAO, were used to generate the dataset for population analysis (S0005, S0155, S0026, S0355, Sw830, Sw2410, Swr1941, Sw632, Sw24, S0228, Sw936, S0097, Sw857, Sw122, Sw2406, IGF1, Sw240, S0090, S0226, Sw72). S0218 was used as a sex marker (data not shown). All loci, except Sw24, did not deviate from Hardy Weinberg equilibrium. Each marker showed an average <em>PIC </em>of 0.779. A total of 260 alleles of length 86 to 272 bp were obtained. Using this dataset, we determined population structure and conservation priorities in the Philippine native pigs. This dataset contains both the raw files (.fsa) and the processed file (.txt). This dataset can be used by colleagues to increase their research coverage and achieve multi-population and multi-country comparisons, especially among Asian indigenous pigs.</p>
Monitoring Plasmodium falciparum and Plasmodium vivax using microsatellite markers indicates limited changes in population structure after substantial transmission decline in Papua New Guinea
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De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea)
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