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22 results for “mtDNA Barcode”
Fig. 36 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Fig. 36. Species of the genus Pseudocellus Platnick, 1980 described from Chiapas, Mexico, including the new species described herein. Star: El Triunfo Biosphere Reserve, El Quetzal Camp, Angel Albino Corzo Municipality. Red circle: Cerro Boquerón, Ejido Boquerón, Motozintla Municipality. Orange circle: Sumidero del Camino, 16 km NE of Comitán. Pink circle: Kolem-chen Cave "Cueva Grande", Chan-kin Reserve, Ocosingo Municipality. Blue circle: San Francisco Cave, La Trinitaria Municipality. Purple circle: Finca Guatimoc, south slope of the Tacaná volcano, 32 km north of Tapachula, near Cacahuatán. Green circle: Las Abejas Cave, San Fernando Municipality.
Fig. 1 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Fig. 1. Neighbour-Joining (NJ) tree with p-distances constructed with COI barcode sequences from different specimens and species of Pseudocellus Platnick, 1980. Colors of the branches indicate species of Pseudocellus already described, red branches indicate the new species. Numbers on the branches represent Bootstrap support values (>50% significant).
Figs 32–35 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 32–35. Pseudocellus giribeti sp. nov. Paratypes (MCZ 80010). 32–33. Deutonymph, habitus, dorsal and ventral views. 34–35. Tritonymph, habitus, dorsal and ventral views. Scale bars = 2 mm.
Figs 20–26 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 20–26. Pseudocellus giribeti sp. nov. Holotype, ♂ (MCZ 80010). 20–22. Right leg III (copulatory apparatus), retrolateral, prolateral and dorsal views. 23. Copulatory apparatus extended, prolateral view. 24. Copulatory apparatus, dorsal view. 25. Copulatory apparatus, prolateral view. 26. Tarsal process, distal half, prodorsal view. Scale bars: 20–22 = 0.5 mm; 23–25 = 0.2 mm; 26 = 0.1 mm.
Figs 7–10 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 7–10. Pseudocellus giribeti sp. nov. Holotype, ♂ (MCZ 80010). 7–8. Opisthosoma, dorsal and ventral views. 9. Tergite XI, median plate (arrow indicates the lateral depression). 10. Pygidium, posterior view. Scale bars: 7–8 = 1 mm; 9 = 0.5 mm; 10 = 0.2 mm.
Figs 3–6 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 3–6. Pseudocellus giribeti sp. nov. Holotype, ♂ (MCZ 80010). 3–4. Habitus, dorsal and ventral views. 5. Carapace, dorsal view. 6. Prosoma, ventral view, showing coxosternal region. Scale bars: 3–4 = 2 mm; 5–6 = 0.5 mm.
Figure 1. A neighbour-joining tree using 604 cytochrome C oxidase sub-unit I in Phylogenetic relationship among slender loris species (Primates, Lorisidae: Loris) in Sri Lanka based on mtDNA CO1 barcoding
Figure 1. A neighbour-joining tree using 604 cytochrome C oxidase sub-unit I (CO1) sequences from 7 different slender loris (Loris) taxas, rooted using slow loris (Nycticebus) sequences deposited in the GenBank.
Data for paper: Genomic data reveals new species and the limits of mtDNA barcode diagnostics to contain a global pest species complex (Diptera: Tephritidae: Dacinae)
<p>Files in this repository:</p><p>"COI_alignment.fas.zip" Zipped file of the FASTA alignment of the COI sequences.</p><p>"COI_IQtree.treefile" Newick treefile resulting from the IQ-tree analysis of the COI alignment.</p><p>"RAD-loci_alignment.nex.zip" Zipped file of the NEXUS alignment of RAD loci of 2295 samples.</p><p>"RAD-loci_IQtree.tre" Newick treefile resulting from the IQ-tree analysis of the RAD-loci alignment.</p><p>"RAD-SNP_alignment.usnps.nex" NEXUS alignment of the RAD-SNP data of 50 samples.</p><p>"RAD-SNP_SNAPP.trees" Set of Newick trees resulting from the BEAST SNAPP analysis.</p>
FIGURES 14–20 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 14–20. Ixchela zapatai sp. nov. Male: 14–15, Habitus, lateral and dorsal views, respectively. 16, Carapace and chelicerae, frontal view. 17, Chelicerae, frontal view. 18, Chelicerae, lateral view. 19–20, Left palp, prolateral and retrolateral views, respectively. FAC, frontal apophysis of chelicerae, PAB: prolateroventral apophysis of bulb, VPP, ventrobasal protuberance of procursus. Scale bars: 0.5 mm (Fig. 17, 18), 1 mm (Figs 16, 19, 20), 2 mm (Figs 14, 15).
FIGURES 25–30. 25–28 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 25–30. 25–28, Living specimens (females) of Ixchela zapatai sp. nov. from type locality. 29–30, Temperate pine-oak forest at 2399 m.a.s.l. at the type locality (red arrow indicates the microhabitat where the specimens of I. zapatai sp. nov were collected).
FIGURES 3–9 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 3–9. Ixchela panchovillai sp. nov. Male: 3–4, Habitus, lateral and dorsal views, respectively. 5, Carapace and chelicerae, frontal view. 6, Chelicerae, frontal view. 7, Chelicerae, lateral view. 8–9, Left palp, prolateral and retrolateral views, respectively. PAB: prolateroventral apophysis of bulb, SAC: sclerotized apophysis of chelicerae, VAF: ventrodistal apophysis of femur, VPP, ventrobasal protuberance of procursus. Scale bars: 0.5 mm (Figs 6, 7), 1 mm (Figs 5, 8, 9), 2 mm (Figs 3, 4).
Data from: DNA barcoding meets molecular scatology: short mtDNA sequences for standardized species assignment of carnivore noninvasive samples
Although species assignment of scats is important to study carnivoran biology, there is still no standardized assay for the identification of carnivores worldwide, which would allow large-scale routine assessments and reliable cross-comparison of results. Here we evaluate the potential of two short mtDNA fragments (ATP6 [126 bp] and COI [187 bp]) to serve as standard markers for the Carnivora. Samples of 66 species were sequenced for one or both of these segments. Alignments were complemented with archival sequences, and analyzed with three approaches (tree-based, distance-based and character-based). Intraspecific genetic distances were generally lower than between-species distances, resulting in diagnosable clusters for 86% (ATP6) and and 85% (COI) of the species. Notable exceptions were recently diverged species, most of which could still be identified using diagnostic characters, uniqueness of haplotypes, or by reducing the geographic scope of the comparison. In silico comparative analyses were also performed with a 110-bp cytochrome b (cytb) segment, whose identification success was lower (70%), possibly due to the smaller number of informative sites and/or the influence of misidentified sequences obtained from GenBank. Finally, we performed case-studies with faecal samples, which supported the suitability of our two focal markers for poor-quality DNA, and allowed an assessment of prey-DNA co-amplification. No evidence of prey DNA contamination was found for ATP6, while some cases were observed for COI and subsequently eliminated by the design of more specific primers. Overall, our results indicate that these segments hold good potential as standard markers for accurate species-level identification in the Carnivora.
Fig. 2 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Fig. 2. Maximum Likelihood gene tree (highest log: -3134.13), constructed with COI barcode sequences of Pseudocellus Platnick, 1980. Colors of the branches indicate species; same colors are in the bars, which represent the different species delimitation methods used for their validation. Red branches + red circle indicate the new species described herein. Numbers below the bars represent the number of species recovered under each species delimitation method (not considering the outgroup: Ricinoides feae (Hansen, 1921)): 1: morphology (M). 2: GMYC. 3: ABGD with recursive partitions (RP). 4: ABGD with initial partitions (IP). 5: bPTP with IB. 6: bPTP with ML. 7: ASAP. Numbers on the branches are Bootstrap support values (>50% significant).
Figs 27–31 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 27–31. Pseudocellus giribeti sp. nov. Paratype, ♀ (MCZ 80010). 27–28. Habitus, dorsal and ventral views. 29–31. Spermathecae, anterior, posterior and lateral views, respectively. Scale bars: 27–28 = 2 mm; 29–31 = 0.2 mm.
Figs 11–19 in COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico
Figs 11–19. Pseudocellus giribeti sp. nov. Holotype, ♂ (MCZ 80010). 11. Cucullus, dorsal view. 12. Left chelicera, dorsal view. 13–14. Right tibia II, prolateral and proventral views. 15–16. Right metatarsus II, prolateral and proventral views. 17. Right femur II, prolateral view. 18. Detail of the movable and fixed claws of the right pedipalp, retrolateral view. 19. Right pedipalp tibia, retrolateral view. Scale bars: 11, 13–17, 19 = 0.5 mm; 12 = 0.2 mm; 18 = 0.1 mm.
Figure 2. A neighbour-joining tree using 604 cytochrome c oxidase subunit I in Phylogenetic relationship among slender loris species (Primates, Lorisidae: Loris) in Sri Lanka based on mtDNA CO1 barcoding
Figure 2. A neighbour-joining tree using 604 cytochrome c oxidase subunit I (CO1) sequences from 7 different slender loris (Loris) taxas found in Sri Lanka with their external appearance.
Data from: DNA barcoding meets molecular scatology: short mtDNA sequences for standardized species assignment of carnivore noninvasive samples
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FIGURES 21–24 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 21–24. Ixchela zapatai sp. nov. Female epigynum: 21, ventral view. 22, dorsal view. 23, frontal view. 24, lateral view. MSE: median septum of epigynum, PP: pore plates. Scale bars: 0.5 mm.
FIGURES 10–13 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURES 10–13. Ixchela panchovillai sp. nov. Female epigynum: 10, ventral view. 11, dorsal view. 12, frontal view. 13, lateral view. MSE: median septum of epigynum, PP: pore plates. Scale bars: 0.5 mm (Figs 12, 13), 1 mm (Figs 10, 11).
FIGURE 2 in COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico
FIGURE 2. Maximum Likelihood gene tree (highest log: -3749.87), constructed with COI barcode sequences of Ixchela. Colors in the branches indicate species; same colors are in the bars, which represent the different species delimitation methods used for their validation. Red branches + blue circles indicate the new species. Numbers below the bars represent the species recovered in each species delimitation method (not considering the outgroup: P. dugesi): 1: morphology (M). 2: neighbor-joining (NJ). 3: GMYC. 4: ABGD with recursive partitions (RP). 5: ABGD with initial partitions (IP). 6: bPTP with IB. 7: bPTP with ML. Numbers above branches are Bootstrap support values (>50% significant), number below are posterior probabilities (PP) support values under BI.
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