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2 results for “nanoscale resolution”
Achieving Nanoscale Resolution with EBSD
<p>Struggling to map nanoscale features in your microstructures with EBSD? Perhaps this video can help... (Case study: indexing the β phase in Ti-6Al-4V).</p> <p>Original research paper: https://doi.org/10.1016/j.matchar.2022.112371</p> <p>Research credits: A. E. Davis, X. Zeng, R. Thomas, J. R. Kennedy, J. Donoghue, A. Gholinia, P. B. Prangnell.</p> <p>Video credits: Produced, written, and performed by Alec E. Davis. Recorded by Alec E. Davis, Jack Donoghue, and Vivek Sahu.</p> <p>This work was supported by grants: Lightform (EPSRC EP/R001715/1), NEWAM (EPSRC EP/R027218/1), and Henry Royce Institute for Advanced Materials (EPSRC EP/R00661X/1, EP/S019367/1, EP/P025021/1, and EP/P025498/1). Alec E. Davis is also appreciated for equipment loan from the @materialsavclub.</p> <p>Commercial EBSD indexing software links:</p> <p>Oxford Instruments: https://www.oxinst.com/blogs/pattern-matching-a-paradigm-shift-for-ebsd EDAX: https://www.edax.com/resources/application-notes/spherical-indexing</p>
Corrected super-resolution microscopy enables nanoscale imaging of auto-fluorescent lung macrophages
<p>Observing the cell surface and underlying cytoskeleton at nanoscale resolution using super-resolution microscopy has enabled many insights into cell signalling and function. However, the nanoscale dynamics of tissue-specific immune cells have been relatively little studied. Tissue macrophages, for example, are highly auto-fluorescent, severely limiting the utility of light microscopy. Here, we report a correction technique to remove auto-fluorescent noise from Stochastic Optical Reconstruction Microscopy (STORM) datasets. Simulations identified a moving median filter as an accurate and robust correction technique. Using this, we were able to visualise lung macrophages activated through Fc receptors by antibody-coated glass slides. Accurate, nanoscale quantification of macrophage morphology revealed that activation induced the formation of cellular protrusions tipped with MHC class I protein. These data are consistent with a role for lung macrophage protrusions in antigen presentation. We further show that the tetraspanin and extracellular vesicle (EV) marker CD81 appears in ring-shaped structures (mean diameter 93 ± 50 nm) at the surface of activated lung macrophages, likely marking the secretion of extracellular vesicles. Moreover, this correction method for super-resolution microscopy is widely applicable to other challenging biological samples.</p>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.