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Dataset results
123 results for “neuronal culture”
Multielectrode array recordings of spontaneous activity in developing neuronal cultures
<p>These data are to accompany the following paper:</p> <p>Charlesworth P, Morton A, Eglen SJ, Komiyama NH, Grant SGN (2015) Canalization of genetic and pharmacological perturbations in developing primary neuronal activity patterns. Neuropharmacology</p> <p>Available at: http://dx.doi.org/10.1016/j.neuropharm.2015.07.027.</p> <p> </p> <p>For further details about the data, please see h<span>ttp://damtp.cam.ac.uk/user/sje30/papers/charlesworth2015_data.pdf</span></p>
Virtual Screening and Testing of GSK-3 Kinase Inhibitors Using human SH-SY5Y Neuronal cells Expressing Tau Folding Reporter and Mouse Hippocampal Primary Neuron Culture Under Tau Cytotoxicity
<p>Supplementary Figure S1. for IJMS</p>
Patch clamp recording from rat hippocampal neuron culture using bulk and NEA chip
<p>Patch-clamp, in current-clamp mode, recordings from rat hippocampal neuron culture using stimulated nano electrode array chip in bulk and low-density NEA chip. All the uncompressed data are in easy-to-use .mat files divided for type of chip (Bulk and NEA); type of coating material and type of stimulation (only for Bulk).</p>
Data from: Extracellular matrix-associated gene expression in adult sensory neurons cultured on laminin substrates
Background: In our previous investigations of the role of the extracellular matrix (ECM) in promoting neurite growth we have observed that a permissive laminin (LN) substrate stimulates differential growth responses in subpopulations of mature dorsal root ganglion (DRG) neurons. DRG neurons expressing Trk and p75 receptors grow neurites on a LN substrate in the absence of neurotrophins, while isolectin B4-binding neurons (IB4+) do not display significant growth under the same conditions. We set out to determine whether there was an expression signature of the LN-induced neurite growth phenotype. Using a lectin binding protocol IB4+ neurons were isolated from dissociated DRG neurons, creating two groups - IB4+ and IB4-. A small-scale microarray approach was employed to screen the expression of a panel of ECM-associated genes following dissociation (t=0) and after 24 hr culture on LN (t=24LN). This was followed by qRT-PCR and immunocytochemistry of selected genes. Results: The microarray screen showed that 36 of the 144 genes on the arrays were consistently expressed by the neurons. The array analyses showed that six genes had lower expression in the IB4+ neurons compared to the IB4- cells at t=0 (CTSH, Icam1, Itgβ1, Lamb1, Plat, Spp1), and one gene was expressed at higher levels in the IB4+ cells (Plaur). qRT-PCR was carried out as an independent assessment of the array results. There were discrepancies between the two methods, with qRT-PCR confirming the differences in Lamb1, Plat and Plaur, and showing decreased expression of AdamTs1, FN, and Icam in the IB4+ cells at t=0. After 24 hr culture on LN, there were no significant differences detected by qRT-PCR between the IB4+ and IB4- cells. However, both groups showed upregulation of Itgβ1 and Plaur after 24 hr on LN, the IB4+ group also had increased Plat, and the IB4- cells showed decreased Lamb1, Icam1 and AdamTs1. Further, the array screen also detected a number of genes (not subjected to qRT-PCR) expressed similarly by both populations in relatively high levels but not detectably influenced by time in culture (Bsg, Cst3, Ctsb, Ctsd, Ctsl, Mmp14, Mmp19, Sparc. We carried out immunohistochemistry to confirm expression of proteins encoded by a number of these genes. Conclusions:Our results show that 1B4+ and IB4- neurons differ in the expression of several genes that are associated with responsiveness to the ECM prior to culturing (AdamTs1, FN, Icam1, Lamb1, Plat, Plaur). The data suggest that the genes expressed at higher levels in the IB4- neurons could contribute to the initial growth response of these cells in a permissive environment and could also represent a common injury response that subsequently promotes axon regeneration. The differential expression of several extracellular matrix molecules (FN, Lamb1, Icam) may suggest that the IB4- neurons are capable of maintaining /secreting their local extracellular environment which could aid in the regenerative process. Overall, these data provide new information on potential targets that could be manipulated to enhance axonal regeneration in the mature nervous system.
Effect of 3D Synthetic Microscaffold Nichoid on the Morphology of Cultured Hippocampal Neurons and Astrocytes
<p>The dataset contains the raw data and images of the paper.</p>
2D cortical human neuron from ipsc - cultures and electrical activiy based on HD-MEA
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Dataset related to article "Neonicotinoid pesticides affect developing neurons in experi-mental mouse models and in human induced pluripotent stem cell (iPSC)-derived neural cultures and organoids"
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Neonicotinoid pesticides affect developing neurons in experi-mental mouse models and in human induced pluripotent stem cell (iPSC)-derived neural cultures and organoids
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Data from: Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons
Amyloid aggregates of the protein α-synuclein (αS) called Lewy Bodies (LB) and Lewy Neurites (LN) are the pathological hallmark of Parkinson's disease (PD) and other synucleinopathies. We have previously shown that high extracellular αS concentrations can be toxic to cells and that neurons take up αS. Here we aimed to get more insight into the toxicity mechanism associated with high extracellular αS concentrations (50-100 μM). High extracellular αS concentrations resulted in a reduction of the firing rate of the neuronal network by disrupting synaptic transmission, while the neuronal ability to fire action potentials was still intact. Furthermore, many cells developed αS deposits larger than 500 nm within five days, but otherwise appeared healthy. Synaptic dysfunction clearly occurred before the establishment of large intracellular deposits and neuronal death, suggesting that an excessive extracellular αS concentration caused synaptic failure and which later possibly contributed to neuronal death.
Data from: Extracellular matrix-associated gene expression in adult sensory neurons cultured on laminin substrates
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Data from: Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons
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Bulk transcriptome analysis of Myt1l mutant mouse neuron primary cultures.
GEO Series GSE217554. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
Single-cell transcriptional and functional analysis of human dopamine neurons in 3D fetal ventral midbrain organoid like cultures
GEO Series GSE192405. Homo sapiens. 13 samples. Type: Expression profiling by high throughput sequencing.
Co-cultures of enteric neurons and T cells
GEO Series GSE164577. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
Identification of activity-dependent gene expression profiles in cultured rat cortical neurons
GEO Series GSE6254. Rattus norvegicus. 11 samples. Type: Expression profiling by array.
Alteration of gene expression profile of cerebelllar neuron-enriched cultures from neonatal rats by nicotine, acetamiprid, or imidacloprid treatment [imidacloprid]
GEO Series GSE80653. Rattus norvegicus. 12 samples. Type: Expression profiling by array.
Networks of cultured iPSC-derived neurons reveal the human synaptic activity-regulated adaptive gene program
GEO Series GSE88773. Mus musculus; Homo sapiens. 22 samples. Type: Expression profiling by high throughput sequencing.
Identification of extensive cellular diversity and maturation of active neuronal networks in long-term cultures of human brain organoids
GEO Series GSE86153. Homo sapiens. 2 samples. Type: Expression profiling by high throughput sequencing.
Alteration of gene expression profile of cerebelllar neuron-enriched cultures from neonatal rats by nicotine, acetamiprid, or imidacloprid treatment
GEO Series GSE80656. Rattus norvegicus. 36 samples. Type: Expression profiling by array.
Aberrant neurodevelopment in human iPS cell-derived astrocyte-neuron co-culture model of Alexander disease
GEO Series GSE261110. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.