Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
21
datasets available to search
ShareScore release 0.7.1
Dataset results
21 results for “non-lethal”
The FAIR-Device - a non-lethal and generalist semi-automatic Malaise trap for insect biodiversity monitoring: Proof of concept - Supplementary Material
<h3>Abstract</h3> <p>Field monitoring plays a crucial role in understanding insect dynamics within ecosystems. It facilitates pest distribution assessment, control measure evaluation, and prediction of pest outbreaks. Additionally, it provides important information on bioindicators with which the state of biodiversity and ecological integrity in specific habitats and ecosystems can be accurately assessed. However, traditional monitoring systems can present various difficulties, leading to a limited temporal and spatial resolution of the obtained information. Despite recent advancements in automatic insect monitoring traps, also called e-traps, most of these systems focus exclusively on studying agricultural pests, rendering them unsuitable for monitoring diverse insect populations. To address this issue, we introduce the Field Automatic Insect Recognition (FAIR)-Device, a novel non-lethal field tool that relies on semi-automatic image capture and species identification using artificial intelligence via the iNaturalist platform. Our objective was to develop an automatic, cost-effective, and non-specific monitoring solution capable of providing high-resolution data for assessing insect diversity. During a 26-day proof-of-concept evaluation, the FAIR-Device recorded 24.8 GB of video, identifying 431 individuals from 9 orders, 50 families, and 69 genera. While improvements are possible, our device demonstrated potential as a cost-effective, non-lethal tool for monitoring insect biodiversity. Looking ahead, we envision new monitoring systems such as e-traps as valuable tools for real-time insect monitoring, offering unprecedented insights for ecological research and agricultural practices.</p> <h3>Description of the data and file structure</h3> <p>This repository complements the publication <a href="https://www.biorxiv.org/content/10.1101/2024.03.22.586299v2" target="_blank" rel="noopener">"The FAIR-Device - a non-lethal and generalist semi-automatic Malaise trap for insect biodiversity monitoring: Proof of concept".</a> It contains the result data from the proof of concept field test of V1.0 of the FAIR-Device, conducted between July and August 2021 at the Thünen Institute of Agricultural Technology in Braunschweig. The repository comprises three compressed files (.zip):</p> <ul> <li><strong>FAIR-D_captures.zip</strong>: <ul> <li>Video captures from the field tests organized by recording day.</li> <li>Filenames indicating recording time (hh-mm-ss).</li> </ul> </li> </ul> <ul> <li><strong>FAIR-D_Tables&Code.zip</strong>: <ul> <li>Processed results from the obtained image captures, organized into: <ul> <li><strong>Monitoring2021_TotalPeriod.xlsx: </strong>Result table with taxonomic classifications, data analysis, and charts.</li> <li><strong>iNat_observations.xlsx: </strong>iNaturalist reviews analysis table.</li> <li><strong>R: </strong>code for generating article graphics.</li> </ul> </li> </ul> </li> </ul> <ul> <li><strong>FAIR-D_V1.0_3D_Models.zip</strong>: <ul> <li>Complete 3D design of the FAIR-D V1.0 in .stl format, organized into: <ul> <li><strong>3D_print_parts</strong>: 3D-printable parts with spatial coordinates for correct positioning in CAD software.</li> <li><strong>other_3Dparts</strong>: Non-printable parts, also in .stl format with spatial coordinates.</li> </ul> </li> </ul> </li> </ul> <p> </p> <p>NOTE: For visualizing the videos, we recommend <strong>VLC media player</strong> - <a href="https://www.videolan.org/vlc/">https://www.videolan.org/vlc/</a> </p>
Non-lethal fungal infection could reduce aggression towards strangers in ants
<p>Many parasites interfere with the behaviour of their hosts. In social animals, such as ants, parasitic interference can cause changes on the level of the individual and also on the level of the society. The ant-parasitic fungus <em>Rickia</em> <em>wasmannii</em> influences the behaviour of <em>Myrmica</em> ants by expanding the host's nestmate recognition template, thereby increasing the chance of the colony accepting infected non-nestmates. Infected ants consistently show an increase of the alkane tricosane (n-C23) in their cuticular hydrocarbon profiles. Although experimental application of single compounds often elicits aggression towards manipulated ants, we hypothesized that the increase of n-C23 might underlie the facilitated acceptance of infected non-nestmates. To test this, we mimicked fungal infection in <em>M</em>. <em>scabrinodis</em> by applying synthetic n-C23 to fresh ant corpses and observed the reaction of infected and uninfected workers to control and manipulated corpses. Infected ants appeared to be more peaceful towards infected but not uninfected non-nestmates. Adding n-C23 to uninfected corpses resulted in reduced aggression in uninfected ants. This supports the hypothesis that n-C23 acts as a 'pacifying' signal. Our study indicates that parasitic interference with the nestmate discrimination of host ants might eventually change colony structure by increasing genetic heterogeneity in infected colonies.</p>
Non-lethal fungal infection could reduce aggression towards strangers in ants
Open the record for dataset details and reuse information.
Digesta and Plasma Metabolomics of Rainbow Trout Strains with Varied Tolerance of Plant-Based Diets Highlights Potential for Non-Lethal Assessments of Enteritis Development
<p>The replacement of fishmeal in aquafeeds is essential to the sustainability of aquaculture. Besides the procurement of alternative protein sources, fish can also be selected for better performance on plant-based alternative diets. Rainbow trout (<em>Oncorhynchus mykiss</em>) is one such species in which the strain ARS-<em>Sel</em>has been selected for higher growth and enhanced utilization when fed soy-based diets. The aim of this study was to compare fish growth, and plasma and digesta metabolomes between the ARS-<em>Sel</em>and two commercial strains (CS-1 and CS-2), when fed a plant-protein diet (PM) and a fishmeal-based diet (FM) and correlate them with the onset of enteritis. An NMR-metabolomics approach was taken to assess plasma and digesta metabolite profiles. Diet and strain showed significant effects on fish growth, with the ARS-<em>Sel</em>fish receiving the PM diet reaching the highest final weight at sampling. Multivariate analysis revealed differences between plasma metabolite profiles of ARS-<em>Sel</em>and CS (CS-1 considered together with CS-2) PM-fed groups in the early stages of the enteritis development, which was confirmed by a histological approach. In digesta, no differences were observed between groups. As reported in previous studies the ARS-<em>Sel</em>strain performed better than the commercial strains when fed the PM diet. </p>
Wing: A suitable non-lethal tissue type for repeatable and rapid telomere length estimates in bats
Telomeres are used increasingly in ecology and evolution as biomarkers for ageing and environmental stress, and are typically measured from DNA extracted from non-lethally sampled blood. However, obtaining blood is not always possible in field conditions and only limited amounts can be taken from small mammals, such as bats, which moreover lack nucleated red blood cells and hence yield relatively low amounts of DNA. As telomere length can vary within species according to age and tissue, it is important to determine which tissues serve best as a representation of the organism as a whole. Here, we investigated whether wing tissue biopsies, a rapid and relatively non-invasive tissue collection method, could serve as a proxy for other tissues when measuring relative telomere length (rTL) in the Egyptian fruit bat (Rousettus aegyptiacus). Telomeres were measured from blood, brain, heart, kidney, liver lung, muscle and wing, and multiple wing biopsies were taken from the same individuals to determine intra-individual repeatability of rTL measured by using qPCR. Wing rTL correlated with rTL estimates from most tissues apart from blood. Blood rTL was not significantly correlated with rTL from any other tissue. Blood and muscle rTL were significantly longer compared with other tissues, while lung displayed the shortest rTLs. Individual repeatability of rTL measures from wing tissue was high (>76%). Here we show the relationships between tissue telomere dynamics for the first time in a bat, and our results provide support for the use of wing tissue for rTL measurements.
Data from: Mortality and lamb body mass growth in free-ranging domestic sheep – environmental impacts including lethal and non-lethal impacts of predators
The management and recovery of large predator populations in areas where human persecution has driven them to ecological extinction requires a solid understanding of the effects of both predation and food limitation on prey populations. We used 11 yr of data on reported losses among 17.3 million free-ranging sheep Ovis aries in the Norwegian farming industry to elucidate the relative roles of climate, vegetation characteristics, sheep densities, lamb body mass and densities of predators and alternative prey on the number of lambs and ewes lost on summer pastures. We first examined whether predator densities predicted autumn lamb body mass through possible impacts of predators on body growth (non-lethal effects) but found no evidence for such effects in our study system. This might be due to weak anti-predator behavioral responses in domesticated sheep. However, autumn lamb body mass was predicted by both sheep density and winter and spring weather conditions, probably through food availability. Losses of both lambs and ewes were positively and strongly related to the density of Eurasian lynx Lynx lynx, wolverine Gulo gulo and brown bear Ursus arctos. In addition, food availability and spring weather conditions were associated to losses of lambs, while precipitation in May predicted losses of ewes. There was little evidence for interaction effects of predator species on losses, suggesting that most of the effects of the predators were additive to each other. Given the strong effect of predator densities on sheep losses, we conclude that changing livestock husbandry practices towards a system that actively protects sheep and/or active management of predator densities may be necessary to reduce sheep losses where predators are recolonizing.
Raw data for Urine DNA (uDNA) as a non-lethal method for endoparasite biomonitoring: development and validation
<p>Changes in environmental conditions alter host-parasite interactions, raising the need for effective epidemiological surveillance. Developing operational, accurate, and cost-effective methods to assess individual infection status and potential for pathogen spread is a prerequisite to anticipate future disease outbreaks in wild populations. For endoparasites, effective detection of infections usually relies on host-lethal approaches, which are barely compatible with wildlife conservation objectives. Here, we used the brown trout (<i>Salmo trutta</i>) - <i>Tetracapsuloides bryosalmonae</i> host-parasite system to develop a non-lethal method for endoparasite infection detection, hereafter called "uDNA" for urine DNA. The uDNA diagnostic test is based on the amplification of endoparasite DNA from host urine. We sampled wild fish (N = 111) from eight sites, let them excrete in individual buckets filled with mineral water and performed parasite DNA amplification from water filtration. We compared the results of the uDNA diagnostic test for host infection status and parasite load to those from kidney samples (the current standard method). uDNA was sensitive in determining host infection status (even for infected hosts showing no sign of the disease), since up to 90% of fish individuals were correctly assigned to their infection status. The quantity of uDNA detected from the hosts depended on the sampling sites, suggesting a spatial variation in the parasite spread. uDNA was positively, but weakly correlated with parasite load in the kidney. This correlation depended on the severity of macroscopic lesions caused by the disease, and was negative in fish with severely damaged kidney, likely due to impaired urine excretion. The uDNA approach provides novel avenues to non-lethally infer infection parameters from wildlife populations at large spatial scales. By targeting parasite transmission stage, uDNA is also valuable to get insights on the parasite fitness and the ecological and evolutionary dynamics of this host-parasite interaction.</p>
Data from: Non-lethal sampling for assessment of mitochondrial function does not affect metabolic rate and swimming performance
Open the record for dataset details and reuse information.
Data from: Mortality and lamb body mass growth in free-ranging domestic sheep – environmental impacts including lethal and non-lethal impacts of predators
Open the record for dataset details and reuse information.
Wing: A suitable non-lethal tissue type for repeatable and rapid telomere length estimates in bats
Open the record for dataset details and reuse information.
Raw data for Urine DNA (uDNA) as a non-lethal method for endoparasite biomonitoring: development and validation
Open the record for dataset details and reuse information.
Broadening the ecology of fear: non-lethal effects arise from diverse responses to predation and parasitism
<p><span>The ecology of fear demonstrates how prey responses to avoid predation cause non-lethal effects at all ecological scales. Parasites also elicit defensive responses in hosts with associated non-lethal effects, which raises the longstanding, yet unresolved question of how non-lethal effects of parasites compare with those of predators. We developed a framework for systematically answering this question for all types of predator and parasite systems. Our framework predicts that trait responses and their non-lethal effects should be strongest from predators and parasites that do not kill individuals to feed on them, but which nevertheless damage fitness. Analysing trait response data on amphibians, which have been well-studied for this area of research, showed that individuals generally responded more directly to short-term predation risks than to parasitism. Apart from studies using amphibians, there have been few direct comparisons of responses to predation and parasitism, and none have incorporated responses to micropredators, parasitoids, or parasitic castrators, or examined their long-term consequences. Addressing these and other data gaps highlighted by our general framework can advance the field toward understanding how non-lethal effects shape real food webs, which contain multiple predator and parasite species. </span></p>
Data from: A non-lethal sampling method to obtain, generate and assemble whole-blood transcriptomes from small, wild mammals
The acquisition of tissue samples from wild populations is a constant challenge in conservation biology, especially for endangered species and protected species where nonlethal sampling is the only option. Whole blood has been suggested as a nonlethal sample type that contains a high percentage of bodywide and genomewide transcripts and therefore can be used to assess the transcriptional status of an individual, and to infer a high percentage of the genome. However, only limited quantities of blood can be nonlethally sampled from small species and it is not known if enough genetic material is contained in only a few drops of blood, which represents the upper limit of sample collection for some small species. In this study, we developed a nonlethal sampling method, the laboratory protocols and a bioinformatic pipeline to sequence and assemble the whole blood transcriptome, using Illumina RNA-Seq, from wild greater mouse-eared bats (Myotis myotis). For optimal results, both ribosomal and globin RNAs must be removed before library construction. Treatment of DNase is recommended but not required enabling the use of smaller amounts of starting RNA. A large proportion of protein-coding genes (61%) in the genome were expressed in the blood transcriptome, comparable to brain (65%), kidney (63%) and liver (58%) transcriptomes, and up to 99% of the mitogenome (excluding D-loop) was recovered in the RNA-Seq data. In conclusion, this nonlethal blood sampling method provides an opportunity for a genomewide transcriptomic study of small, endangered or critically protected species, without sacrificing any individuals.
Data for impact of “non-lethal” tarsal clipping on bumble bees (Bombus vosnesenskii) may depend on queen stage and worker size
Open the record for dataset details and reuse information.
Broadening the ecology of fear: non-lethal effects arise from diverse responses to predation and parasitism
Open the record for dataset details and reuse information.
Data from: A non-lethal sampling method to obtain, generate and assemble whole-blood transcriptomes from small, wild mammals
Open the record for dataset details and reuse information.
Identification of biological processes that distinguish lethal from non-lethal influenza infection
GEO Series GSE42638. Mus musculus. 138 samples. Type: Expression profiling by array.
Transcriptomic comparison of 5 cell types during lethal and non-lethal influenza infection
GEO Series GSE42639. Mus musculus. 75 samples. Type: Expression profiling by array.
Time series of Balb/c spleen infected with Plasmodium yoelii non-lethal vs lethal strains
GEO Series GSE17603. Mus musculus. 12 samples. Type: Expression profiling by array.
Ecological transcriptomics: a non-lethal sampling approach for endangered fire salamanders
GEO Series GSE70054. Salamandra salamandra. 24 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.