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24 results for “nuclear microsatellites”
Nuclear genetic diversity and structure of Anastrepha ludens wild populations evidenced by microsatellite markers
<p class="MsoNormal"><span>The Mexican fruit fly, <em>Anastrepha ludens </em>is an important pest that causes widespread damage to a number of fruit crops in Mexico. The Sterile Insect Technique (SIT) is commonly used for its control. However, the existence of natural barriers can give rise to a population structure in neutral loci and possibly behavioral or adaptive traits that interfere with SIT. For this reason, it is important to understand the genetic diversity and structure of<em> A. ludens </em>populations and to better understand the evolutionary ecology and population processes in view of possible expansions and possible host shifts due to climate change. We genotyped nine nuclear DNA (nDNA) microsatellite loci among fruit fly populations collected from five biogeographic areas within Mexico, Mexican Plateau, the Northeastern Coastal Plain, the Pacific Coast, the Gulf Coast of Mexico, the Soconusco and a laboratory strain. The nuclear genetic diversity was moderate (from <em>H</em>e = 0.34 to <em>H</em>e = 0.39) within the wild mexfly population. We found that populations were clustered in three genetic groups (<em>K</em>=3). The diversity and genetic structure of <em>A. ludens</em> are produced by environmental and geological conditions as well as local conditions like anthropogenic perturbation which would produce population expansion and the existence of possible predators that would affect the population density. Gene flow showed recent migration among populations. The laboratory strain showed less diversity than the wild samples. Large values of current and ancestral population size suggest high resistance to climatic changes, probably due to biological attributes, such as its polyphagous, multivoltine and high dispersal characteristics. In particular ecosystem fragmentation and perturbation as well as the existence of new plant hosts all of which would probably increase the abundance of flies.</span></p>
Genotype data of 10 nuclear microsatellite loci for 30 Quercus acutissima populations in China
<p>This dataset includes genotype data of 10 nuclear microsatellite loci for 707 individuals of Quercus acutissima from 30 natural populations in China.</p>
Microsatellite data, chloroplast and nuclear rRNA sequences of Avicennia marina from Vietnam, Malaysia, and The Philippines
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Nuclear genetic diversity and structure of Anastrepha ludens wild populations evidenced by microsatellite markers
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Data from: Development of nuclear microsatellite loci and mitochondrial single nucleotide polymorphisms for the natterjack toad, Bufo (Epidalea) calamita (Bufonidae), using next generation sequencing and Competitive Allele Specific PCR (KASPar)
Amphibians are undergoing a major decline worldwide and the steady increase in the number of threatened species in this particular taxa highlights the need for conservation genetics studies using high-quality molecular markers. The natterjack toad, Bufo (Epidalea) calamita, is a vulnerable pioneering species confined to specialized habitats in Western Europe. To provide efficient and cost-effective genetic resources for conservation biologists, we developed and characterized 22 new nuclear microsatellite markers using next-generation sequencing. We also used sequence data acquired from Sanger sequencing to develop the first mitochondrial markers for KASPar assay genotyping. Genetic polymorphism was then analyzed for 95 toads sampled from 5 populations in France. For polymorphic microsatellite loci, number of alleles and expected heterozygosity ranged from 2 to 14 and from 0.035 to 0.720, respectively. No significant departures from panmixia were observed (mean multilocus F IS = −0.015) and population differentiation was substantial (mean multilocus F ST = 0.222, P < 0.001). From a set of 18 mitochondrial SNPs located in the 16S and D-loop region, we further developed a fast and cost-effective SNP genotyping method based on competitive allele-specific PCR amplification (KASPar). The combination of allelic states for these mitochondrial DNA SNP markers yielded 10 different haplotypes, ranging from 2 to 5 within populations. Populations were highly differentiated (G ST = 0.407, P < 0.001). These new genetic resources will facilitate future parentage, population genetics and phylogeographical studies and will be useful for both evolutionary and conservation concerns, especially for the set-up of management strategies and the definition of distinct evolutionary significant units.
Data from: Nuclear microsatellite and mitochondrial DNA analyses reveal the regional genetic structure and phylogeographical history of a sanguivorous land leech, Haemadipsa japonica, in Japan
Recent molecular studies have indicated that phylogeographical history of Japanese biota is likely shaped by geohistory along with biological events, such as distribution shifts, isolation, and divergence of populations. However, the genetic structure and phylogeographical history of terrestrial Annelida species, including leech species, are poorly understood. Therefore, we aimed to understand the genetic structure and phylogeographical history across the natural range of Haemadipsa japonica, a sanguivorous land leech species endemic to Japan, by using nine polymorphic nuclear microsatellites (nSSR) and cytochrome oxidase subunit one (COI) sequences of mitochondrial DNA (mtDNA). Analyses using nSSR revealed that H. japonica exhibited a stronger regional genetic differentiation among populations (G'ST = 0.77) than other animal species, probably because of the low mobility of land leech. Analyses using mtDNA indicated that H. japonica exhibited two distinct lineages (A and B), which were estimated to have diverged in the middle Pleistocene and probably because of range fragmentation resulting from climatic change and glacial and interglacial cycles. Lineage A was widely distributed across Japan, and lineage B was found in southwestern Japan. Analyses using nSSR revealed that lineage A was roughly divided into two population groups (i.e., northeastern and southwestern Japan); these analyses also revealed a gradual decrease in genetic diversity with increasing latitude in lineage A and a strong genetic drift in populations of northeastern Japan. Combined with the largely unresolved shallow polytomies from the mtDNA phylogeny, these results implied that lineage A may have undergone a rapid northward migration, probably during the Holocene. Then, the regional genetic structure with local unique gene pools may have been formed within each lineage because of the low mobility of this leech species.
Data from: Combining chloroplast and nuclear microsatellites to investigate origin and dispersal of New World sweet potato landraces
We analyzed a representative collection of New World sweet potato landraces (329 accessions from Mexico to Peru) with both chloroplast and nuclear microsatellite markers. Both kinds of markers supported the existence of two geographically restricted genepools, corresponding to accessions from the north-western part of South America and accessions from the Caribbean and Central America super-region. Our conservative cpSSRs markers revealed that the divergence between the two haplotype groups is associated with numerous mutation events concerning various markers, supporting the idea that this divergence may be ancient, predating domestication. For both kind of markers, we found no significant difference in diversity between the two genepools and detected region-specific alleles in both groups. Previous studies have favoured the hypothesis of a single domestication of this crop. Our analysis suggests at least two independent domestications, in Meso-America and in the north-western part of South America. Sweet potato was then dispersed from these centres throughout tropical America. Comparison of nuclear and chloroplast data suggests that exchanges of clones and sexual reproduction were both important processes in landrace diversification in this clonally propagated crop. Our analysis provides useful tools for rationalizing the conservation and use of germplasm collections.
Drakaea glyptodon nuclear microsatellite and chloroplast haplotype data
<p class="CxSpFirst">Many orchids are characterized by small, patchily distributed populations. Resolving how they persist is important for understanding the ecology of this hyper-diverse family, many members of which are of conservation concern. <span>Ten</span> populations of the common terrestrial orchid <i>Drakaea glyptodon</i> from Southwest Australia were genotyped with ten nuclear and five chloroplast SSR markers. Levels and partitioning of genetic variation, and effective population sizes (<i>N</i><sub>e</sub>), were estimated. Spatial genetic structure of nuclear diversity, together with chloroplast data, are used to infer the effective number of seed parents per population. We found high genetic diversity, <i>N</i><sub>e</sub> values that generally exceed predictions based on the number of flowering individuals, and moderate levels of gene flow. Two populations were founded by < 5 colonists suggesting some populations are colonized by few seeds, with growth largely resulting from <i>in situ</i> recruitment. A value of 3.65 for <i>m</i><sub>p </sub>/<i>m</i><sub>s</sub> indicates that pollinators play a greater role than seed in introducing genetic diversity to populations via gene flow. Our results highlight that <i>D. glyptodon</i> is highly effective at persisting in patchily distributed populations. However, it is important to examine how insights from this common, widespread species transfer to species that are rare and/or occur in fragmented landscapes.</p>
Orchis nuclear microsatellite data
<p><b>Premise of the study </b>The genetic structure of hybrid zones provides an insight into the potential for gene flow to occur between plant taxa. Four closely related European orchid species (<i>Orchis anthropophora</i>, <i>O. militaris</i>, <i>O. purpurea</i> and <i>O. simia</i>) hybridize when they co-occur. We aimed to characterize patterns of hybridization in <i>O. militaris – O. purpurea</i>, <i>O. purpurea – O. simia</i> and <i>O. anthropophora – O. simia</i> hybrid zones using molecular and morphological data.</p> <p><b>Methods </b>We used 11 newly isolated nuclear microsatellites to genotype 695 individuals collected from seven hybrid zones and six allopatric parental populations in France. Geometric morphometric analysis was conducted using 15 labellum landmarks to capture the main aspects of petal shape.</p> <p><b>Key Results </b>Backcrossing was asymmetric towards <i>O. militaris</i> in multiple <i>O. militaris – O. purpurea</i> hybrid zones. Hybrids in <i>O. purpurea – O. simia</i> and <i>O. anthropophora – O. simia</i> hybrid zones were largely limited to F1 and F2 generations, but further admixture had occurred. These patterns were reflected in labellum geometric morphometric data which correlated strongly with nuclear microsatellite data in all three species combinations.</p> <p><b>Conclusions </b>The co-existence of parental and admixed individuals in these <i>Orchis</i> hybrid zones implies they are likely to be tension zones being maintained by a balance between gene flow into the hybrid zone and selection acting against admixed individuals. The pattern of admixture in the three species combinations suggests intrinsic selection acting on the hybrids is weaker in more closely related taxa.</p>
12 nuclear microsatellite loci scores for 543 adult trees of Tilia cordata in Lithuainia
<p>Genetic signature of the natural genepool of <i>Tilia cordata</i> Mill. in Lithuania: compound evolutionary and anthropogenic effects</p> <p><i>Tilia cordata</i> Mill. is a valuable tree species enriching the ecological values of the coniferous dominated boreal forests in Europe. Following the historical decline, spreading of <i>Tilia</i> sp. is challenged by the elevated inbreeding and habitat fragmentation. We studied the geographical distribution of genetic diversity of <i>Tilia cordata</i> populations in Lithuania. We used 14 genomic microsatellite markers to genotype 543 individuals from 23 wild growing populations. We found that <i>Tilia cordata</i> retained high levels of genetic diversity (population F<sub>is</sub> = 0 to 0.15, H<sub>o </sub>= 0.53 to 0.69, H<sub>e </sub>= 0.56 to 0.75). AMOVA, Bayesian clustering and Monmonier's barrier detection indicate weak but significant differentiation among the populations (F<sub>st</sub> = 0.037***) into geographically interpretable clusters of (a) western Lithuania with high genetic heterogeneity but low genetic diversity, bottleneck effects, (b) relatively higher genetic diversity of <i>Tilia cordata</i> on rich and most soils of midland lowland, and (c) the most differentiated populations on poor soils of the coolest north-eastern highland possessing the highest rare allele frequency but elevated inbreeding and bottleneck effects. Weak genetic differentiation among the <i>Tilia cordata</i> populations in Lithuania implies common ancestry, absence of strong adaptive gradients and effective genetic exchange possible mediated via the riparian networks. A hypothesis on riparian networks as geneflow mediators in <i>Tilia cordata</i> was raised based on results of this study.</p>
Genotype data of 14 nuclear microsatellite loci for 18 Quercus chenii populations in China
<p>This dataset includes genotype data of 14 nuclear microsatellite loci for 419 individuals of Quercus chenii from 18 natural populations in China.</p>
Data from: Development of 15 nuclear EST microsatellite markers for the palaeoendemic conifer Pherosphaera hookeriana (Podocarpaceae)
Premise of the study: Nuclear microsatellite markers were developed for population genetic analysis of the threatened palaeoendemic conifer Pherosphaera hookeriana W. Archer (Podocarpaceae). Methods and Results: Fifteen variable loci were identified showing 1 to 13 alleles per population with seven loci displaying over four alleles in all populations and the average number of alleles per locus ranging from 4.8 to 5.93 per population. The observed heterozygosity per locus varied from 0.00 to 0.91 and overall heterozygosity was markedly consistent between populations (from 0.54 to 0.57). All loci also amplified in the endangered congener P. fitzgeraldii but only five of the loci had more than one allele. Conclusions: These 15 loci are the first microsatellite markers developed in the genus Pherosphaera. These loci will be useful for investigating the species' extant genetic diversity and structure, the impact of past environmental change and the significance of asexual reproduction.
Data from: Combining chloroplast and nuclear microsatellites to investigate origin and dispersal of New World sweet potato landraces
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Data from: Indications for three independent domestication events for the tea plant (Camellia sinensis (L.) O. Kuntze) and new insights into the origin of tea germplasm in China and India revealed by nuclear microsatellites
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Data from: Development of nuclear microsatellite loci and mitochondrial single nucleotide polymorphisms for the natterjack toad, Bufo (Epidalea) calamita (Bufonidae), using next generation sequencing and Competitive Allele Specific PCR (KASPar)
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Data from: Despite introgressive hybridization, North American birches (Betula spp.) maintain strong differentiation at nuclear microsatellite loci
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Drakaea glyptodon nuclear microsatellite and chloroplast haplotype data
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Data from: Nuclear microsatellite and mitochondrial DNA analyses reveal the regional genetic structure and phylogeographical history of a sanguivorous land leech, Haemadipsa japonica, in Japan
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Data from: Development of 15 nuclear EST microsatellite markers for the palaeoendemic conifer Pherosphaera hookeriana (Podocarpaceae)
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12 nuclear microsatellite loci scores for 543 adult trees of Tilia cordata in Lithuainia
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