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1,048 results for “oocyte”
Movies of mouse oocyte maturation in transmitted light
<p>This dataset has been presented in our paper "An interpretable and versatile machine learning approach for oocyte phenotyping", in bioRxiv.</p> <p>It contains 466 movies of mouse oocytes maturation acquired in transmitted light every 3 min. Spatial resolution is 0.227 µm/pixel.</p>
Mass spectrometry raw data for "Proteomics reveals substantial differences between in vitro matured abattoir-derived and in vivo matured oocytes in cattle"
<p><em><span>In vitro</span></em><span> production (IVP) of bovine embryos still has its limitations such as low blastocyst rate and lower embryo quality, resulting in lower pregnancy rates following the transfer of IVP embryos compared to <em>in vivo</em> produced embryos. </span><span>Given these differences in developmental competence, RNA sequencing and microarray technology have been applied to describe the differences in transcriptional activity between <em>in vitro</em> and <em>in vivo</em> produced embryos. All but one of these studies solely utilized oocytes obtained from slaughterhouse material for the <em>in vitro</em> production of embryos, thereby introducing the possibility, that differences between IVP and <em>in vivo</em> embryos are in part attributable to differing sources of oocytes. The aim of the present study was therefore to compare the proteome of oocytes retrieved from slaughterhouse material, with and without a period of <em>in vitro</em> maturation and <em>in vivo</em> matured oocytes obtained from donor cattle following superovulation. <span>For each group the protein pattern of four biological replicates containing ten oocytes each were analyzed via SWATH<sup>TM</sup>-MS.</span></span></p>
The optimal period for oocyte retrieval after the administration of recombinant human chorionic gonadotropin in in vitro fertilization
<p>This is the dataset of the study called "The optimal period for oocyte retrieval after the administration of recombinant human chorionic gonadotropin in in vitro fertilization".</p> <p><strong>Abstract</strong></p> <p>Background</p> <p>Our objective was to investigate the existence of an optimal period for oocyte retrieval in regards to the clinical pregnancy occurrence after the administration of recombinant human chorionic gonadotropin (rhCG) (Ovitrelle®).</p> <p>Methods</p> <p>We studied the digital records of 3362 middle eastern couples who underwent in vitro fertilization (IVF) treatment between 2019 and 2021.</p> <p>Results</p> <p>Through statistical testing, we found that there is a significant positive correlation between the oocyte retrieval period and the clinical pregnancy occurrence up to the 37th hour, where retrieval at the 37th hour was found to provide the most optimal outcome, especially in the case of gonadotropin-releasing hormone agonist (GnRHa) long protocol.</p> <p>Conclusions</p> <p>This cohort study recommends retrieval at hour 37 after ovulation triggering under the described conditions.</p>
Segmentation of membrane of mouse, sea urchin and human oocytes from transmitted light images
<p>This dataset has been presented in our paper "An interpretable and versatile machine learning approach for oocyte phenotyping", in bioRxiv.</p> <p>It contains images acquired in transmitted light with different settings of mouse and human oocytes and sea urchin eggs, with the corresponding ground-truth of the membrane segmentation. Mouse oocyte images were taken before and during oocyte maturation (meiosis I). Some human oocyte images were taken during oocyte maturation (meiosis I), and some are M-II oocytes just after fertilization. Sea urchin images contains both fertilized and unfertilized eggs.</p> <p> </p>
Segmentation of oocyte zona pellucida in transmitted light images (mouse and human)
<p>This dataset has been presented in our paper "An interpretable and versatile machine learning approach for oocyte phenotyping", in bioRxiv.</p> <p>It contains images acquired in transmitted light with different settings of mouse and human oocytes, with the corresponding ground-truth of the zona pellucida segmentation. Mouse oocyte images were taken before and during oocyte maturation (meiosis I). Some human oocyte images were taken during oocyte maturation (meiosis I), and some are M-II oocytes just after fertilization.</p>
F I G U R E A 1 Oocytes. Figure A1 in Reproductive biology of the electric lanternfish Electrona risso (Myctophidae) and the bigscale fishes Melamphaes polylepis and Scopelogadus mizolepis (Melamphaidae)
F I G U R E A 1 Oocytes. Figure A1: Oocytes of female Electrona risso per reproductive phase (see Table 1), photographed with reflected light. Photography by K. Wieben.
Рис. 8. СреЗы череЗ гонады моллюска: А – поперечный среЗ череЗ гонаду самки, Б–Д – фолликулы в гонадах самок (Б, В – Зрелые ооциты круглой формы, готовые к вымету; Г – ооциты в период активного гаметогенеЗа на стадии раннего трофоплаЗматического роста, Д – ооциты каплевидной формы в период преднерестовой стадии при ЗаверШении трофоплаЗматического роста), Е, Ж – поперечные среЗы череЗ гонаду самца, З, И – ацинусы в гонадах самцов (З – преднерестоваЯ стадиЯ, просветы в ацинусах практически отсутствуют, стенки ацинусов не раЗличимы, И – нерестоваЯ стадиЯ, имеютсЯ просветы в ацинусах). МасШтабные линейки 300 мкм (А), 200 мкм (Е), 100 мкм (Ж), 50 мкм (Б–Д, З, И). вя – вакуолиЗированное Ядро, сф – стенка фолликула, вм – вителлиноваЯ мембрана, РО – раЗвиваюЩиесЯ иЗ пелликулы ооциты, пг – ресничный проток гонады, с – сперматоциты, па – просветы в ацинусах. Fig. 8. Sections through the gonads of the mollusk: А – transverse section through the female gonad, Б–Д – ovarian acini, follicles (Б, В – mature round-shaped oocytes ready to be swept out; Г – oocytes in the period of active gametogenesis at the stage of early trophoplasmatic growth, Д – tear-shaped oocytes during the pre-spawning stage at the end of trophoplasmatic growth), Е, Ж – transverse sections through the male gonads, З, И – testicular acini (З – pre-spawning stage, with practically absent gaps in the acini and invisible the acini walls, И – spawning stage, with gaps in the acini). Scale bars 300 µm (A), 200 µm (E), 100 µm (Ж), 50 µm (Б–Д, З, И). вя – vacuolated nucleus, сф – follicle wall, вм – vitelline membrane, РО – developing oocytes arising from a pellicle, пг – ciliated gonadal duct, с – spermatocytes, па – gaps in acini. in Nodularia vladivostokensis (Bivalvia: Unionidae) from Razdolnaya River (Primorye, Russia)
Рис. 8. СреЗы череЗ гонады моллюска: А – поперечный среЗ череЗ гонаду самки, Б–Д – фолликулы в гонадах самок (Б, В – Зрелые ооциты круглой формы, готовые к вымету; Г – ооциты в период активного гаметогенеЗа на стадии раннего трофоплаЗматического роста, Д – ооциты каплевидной формы в период преднерестовой стадии при ЗаверШении трофоплаЗматического роста), Е, Ж – поперечные среЗы череЗ гонаду самца, З, И – ацинусы в гонадах самцов (З – преднерестоваЯ стадиЯ, просветы в ацинусах практически отсутствуют, стенки ацинусов не раЗличимы, И – нерестоваЯ стадиЯ, имеютсЯ просветы в ацинусах). МасШтабные линейки 300 мкм (А), 200 мкм (Е), 100 мкм (Ж), 50 мкм (Б–Д, З, И). вя – вакуолиЗированное Ядро, сф – стенка фолликула, вм – вителлиноваЯ мембрана, РО – раЗвиваюЩиесЯ иЗ пелликулы ооциты, пг – ресничный проток гонады, с – сперматоциты, па – просветы в ацинусах. Fig. 8. Sections through the gonads of the mollusk: А – transverse section through the female gonad, Б–Д – ovarian acini, follicles (Б, В – mature round-shaped oocytes ready to be swept out; Г – oocytes in the period of active gametogenesis at the stage of early trophoplasmatic growth, Д – tear-shaped oocytes during the pre-spawning stage at the end of trophoplasmatic growth), Е, Ж – transverse sections through the male gonads, З, И – testicular acini (З – pre-spawning stage, with practically absent gaps in the acini and invisible the acini walls, И – spawning stage, with gaps in the acini). Scale bars 300 µm (A), 200 µm (E), 100 µm (Ж), 50 µm (Б–Д, З, И). вя – vacuolated nucleus, сф – follicle wall, вм – vitelline membrane, РО – developing oocytes arising from a pellicle, пг – ciliated gonadal duct, с – spermatocytes, па – gaps in acini.
Fig. 6 in Oocyte development and ovarian maturation of the black triggerfish, Melichthys niger (Actinopterygii: Balistidae) in São Pedro e São Paulo Archipelago, Brazil
Fig. 6. Photomicrograph of ovaries sections of black triggerfish at different maturation stages. (a) early maturation, (b) middle maturation, (c) mature, and (d) resting. PVO: previtellogenic oocytes; CAO: cortical alveoli oocytes; VO: vitellogenic oocytes; MO: mature oocyte; and OW: ovary wall.
Fig. 4 in Oocyte development and ovarian maturation of the black triggerfish, Melichthys niger (Actinopterygii: Balistidae) in São Pedro e São Paulo Archipelago, Brazil
Fig. 4. Mean diameter (µm) of oocytes for each oocyte development stage of ovaries of black triggerfish caught in the São Pedro e São Paulo Archipelago, between January 2006 and May 2008. (YC) young cell, (PVO) pre-vitellogenic oocyte, (CAO) cortical alveoli oocytes, (VO) vitellogenic oocytes and (MO) mature oocytes. Asterisks indicate significant differences (Wilcoxon, p <0.05).
Fig. 2 in Oocyte development and ovarian maturation of the black triggerfish, Melichthys niger (Actinopterygii: Balistidae) in São Pedro e São Paulo Archipelago, Brazil
Fig. 2. Photomicrographs of the oogenesis stages of ovaries of black triggerfish caught in the São Pedro e São Paulo Archipelago, between January 2006 and May 2008. (a) YC - young cells, oogonia - OG; (b) PVO - previtellogenic oocytes; (c) CAO - cortical alveoli oocytes; (d) VO - vitellogenic oocytes; (e) MO - mature oocyte; (f) ZP - zona pellucida (radiated). N - nucleus, Nc - nucleolus; FC - follicular cell; LV - lipid vacuole; YG - yolk globules; CA - cortical alveoli.
Figure 1 in Oocyte distribution, ovarian organization, and spawning pattern in Lutjanus griseus
Figure 1. – Phase-specific variation of oocyte size frequency in ovaries from female Lutjanus griseus in the early developing subphase; spawning capable phase; actively spawning subphase; and past-spawner subphase. Oocytes undergoing germinal vesicle breakdown (GVBD) and hydrated (H) were observed during the actively spawning subphase, but their diameter could not be determined since nucleus was not visible. PG = primary growth oocyte (mean diameter = 47.1 ± 15.1 μm, n = 46730); CA = cortical alveolar oocyte (128.3 ± 29.5 μm, n = 3148); Vtg1 = primary vitellogenic oocyte (190.3 ± 28 μm, n = 709); Vtg2 = secondary vitellogenic oocyte (258.32 ± 29 μm, n = 631) and Vtg3 = tertiary vitellogenic oocyte (298.58 ± 28.2 μm, n = 935); OM (GVM) = oocyte undergoing germinal vesicle migration (274.47 ± 21.2 μm, n = 6). Postovulatory follicles were present in ovaries of past-spawner females.
Figure 7 in Sex ratio, oocyte development and spawning season of Mediterranean moray eel (Muraena helena) from the northern coast of Tunisia
Figure 7. – Section of gonad, illustrating the spawning capable reproductive phase of ovary (CA = cortical alveolar oocyte; Vtg3 = tertiary vitellogenic oocyte).
Figure 5 in Sex ratio, oocyte development and spawning season of Mediterranean moray eel (Muraena helena) from the northern coast of Tunisia
Figure 5. – Section of gonad, illustrating the immature phase of ovary (PG = primary growth oocyte; OW = ovarian wall).
Figure 2 in Sex ratio, oocyte development and spawning season of Mediterranean moray eel (Muraena helena) from the northern coast of Tunisia
Figure 2. Seasonal variation of the gonadosomatic index (GSI), hepatosomatic index (HSI) and relative condition factor (CF) of Muraena helena from the northern coast of Tunisia. Bars are mean values (± 2 standard errors).
◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord in Hardly Venus's servant-morphological adaptations of Veneriserva to an endoparasitic lifestyle and its phylogenetic position within Dorvilleidae (Annelida)
◂Fig. 5 Gametogenesis in male and female Veneriserva pygoclava. A–D Semi-thin histological sections of female Veneriserva pygoclava, stained with toluidine blue. A Cross-section of a female Veneriserva. B Close-up of large mature oocytes without discernible nurse cells. C Developing oocytes attached to mesenteries (mes), and oogonia proliferating from the ventral side of the dorsal blood vessel (bv). D Details of vitellogenic oocytes and nurse cells. Arrowheads indicate brownstained yolk platelets and yolk bodies. E Live sperm cells captured in a light micrograph. F–G Cross-sections of male Veneriserva. Note the absence of a gut in the cross-sections. Abbreviations—ac acicula, acr acrosome, bv blood vessel, coe coelomic cavity, mes mesentery, nc nurse cell, nn nurse cell nucleus, nu sperm cell nucleus, Oo oocyte, on oocyte nucleus, sp spermatogonia, vnc ventral nerve cord
Optimal modes for wavefront sensorless adaptive optics. Turbulence- and oocyte-induced phase screens and Mathematica notebooks.
<p>This notebook and phase screens constitute a numerical experiment to calculate the error of the wavefront approximation using first N modes of the a) Zernike and b) Lukosz-Braat polynomials, c) SVD modes obtained with respect to the gradient-dot product, and the d) eigenfunctions of the Laplace operator with the Neumann boundary conditions. It's a complementary material to a paper submitted to Optics Express.</p>
Figure 2 in Oocyte development in Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae): presence of Balbiani body*
Figure 2. Histological sections of ovary including different stage of oocytes stained with Periodic Acid Schiff (PAS). a) PAS positive materials (asterisk) in the oocytes belonging to different developmental stages. b) PAS positive yolk granules covering the vitellogenic (V) and mature oocytes (M) and PAS negative lipid droplets within these oocytes' ooplasm.
Figure 1 in Oocyte development in Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae): presence of Balbiani body*
Figure 1. Developmental stages of oocytes. a) A few vacuoles (asterisks) of oocytes encircled by follicle epithelium (FE) in previtellogenic stage. b) Large nucleus with prominent nucleolus (No) in previtellogenic stage. c) Yolk granules (YG) firstly appearing just beneath the oolemma in early vitellogenic stage. d) Balbiani body (yolk nucleus) (Bb) around germinal vesicle (nucleus) (N) in mid-vitellogenic stage. e) Many yolk granules (YG) within the oocyte in late-vitellogenic stage. f) Oocytes with large yolk granules (YG) in maturation stage.
Figure 4 in Oocyte development in Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae): presence of Balbiani body*
Figure 4. Oosorption of oocytes. a) Oocytes (O) resorbed by hypertrophied follicle cells (H) and space (asterisk) encircled by follicle cells. b) Hypertrophic (H) follicle cells engulfing the oocyte and apoptotic cell with crescent-shaped nucleus (triangled) and apoptotic bodies (encircled).
Figure 3 in Oocyte development in Melanogryllus desertus (Pallas, 1771) (Orthoptera: Gryllidae): presence of Balbiani body*
Figure 3. Histological sections of ovary including different stage of oocytes stained with Bromophenol Blue (BpB). a) BpB positive materials (asterisks) in the oocytes belonging to different developmental stages. Arrow: Balbiani body. b) BpB positive yolk granules covering the vitellogenic (V) and mature oocytes (M) and lipid droplets that do not react with BpB.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.