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Dataset results
16 results for “patch clamp”
Patch-clamp recordings from dorsal raphe neurons
<p>This dataset contains whole-cell electrophysiological recordings (patch-clamp recordings) from three cell types in mice:</p> <ul> <li>serotonin (5-HT) neurons,</li> <li>somatostatin (SOM)-expressing GABA interneurons, and</li> <li>layer 5 pyramidal neurons.</li> </ul> <p>5-HT and GABA neurons were recorded in the dorsal raphe nucleus (DRN), which is the main source of serotonergic input to the forebrain. Together, they make up the majority of the neurons found in the DRN.</p> <p>This dataset can be used to investigate the intrinsic electrophysiological properties of these two types of DRN neurons and contrast them with another abundant and well-studied cell type, the L5 pyramidal neuron.</p> <p>This data was used in <a href="https://doi.org/10.7554/eLife.72951" rel="noopener" title="Temporal derivative computation in the dorsal raphe network revealed by an experimentally-driven augmented integrate-and-fire modeling framework | eLife">our paper</a> describing a spiking neural network model of the dorsal raphe nucleus:</p> <p>Emerson F. Harkin, Michael B. Lynn, Alexandre Payeur, Jean-François Boucher, Léa Caya-Bissonnette, Dominic Cyr, Chloe Stewart, André Longtin, Richard Naud, and Jean-Claude Béïque. Temporal derivative computation in the dorsal raphe network revealed by an experimentally-driven augmented integrate-and-fire modeling framework. eLife, 2023. doi: 10.7554/eLife.72951</p>
Patch-clamp recordings from dorsal raphe neurons
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Data sets comparing Ca(2+) dynamics in whole-cell and β-escin-based perforated patch clamp recordings in adult mouse brain slices.
<p># Hess-et-al-beta-escin-2020<br> Data of the "Data in Brief" article concerning the added buffer approach with beta-escin perforated patch.</p> <p>A joint work by: Simon Hess (`simon.hess@uni-koeln.de`), Christophe Pouzat (`christophe.pouzat@math.unistra.fr`), and Peter Kloppenburg (`peter.kloppenburg@uni-koeln.de`).</p> <p>## Content</p> <p>This repository contains:</p> <p>- Directory `data_whole_cell` contains the experimental data in [HDF5](https://en.wikipedia.org/wiki/Hierarchical_Data_Format) format. The data contains recordings of _Substantia nigra_ dopaminergic neurons recorded in the whole-cell configuration.<br> - Directory `data_beta_escin` contains the experimental data in [HDF5](https://en.wikipedia.org/wiki/Hierarchical_Data_Format) format. The data contains recordings of _Substantia nigra_ dopaminergic neurons recorded in the β-escin perforated patch clamp configuration.</p>
Patch clamp dataset for: A massively parallel assay accurately discriminates between functionally normal and abnormal variants in a hotspot domain of KCNH2
<p><span>Many genes, including <em>KCNH2</em>, contain </span><span>'hotspot' domains associated with a high density of variants associated with disease. This has led to the suggestion that variant location can be used as evidence supporting classification of clinical variants. However, it is not known what proportion of all potential variants in hotspot domains cause loss of function. Here, we have used a massively parallel trafficking assay to characterize all single-nucleotide variants in exon 2 of <em>KCNH2, </em>a known hotspot for variants that cause long QT syndrome type 2 and an increased risk of sudden cardiac death<em>. </em>Forty-two percent of <em>KCNH2</em> exon 2 variants caused at least 50 % reduction in protein trafficking and 65% of these trafficking defective variants exerted a dominant-negative effect when co-expressed with a WT <em>KCNH2</em> allele as assessed using a calibrated patch clamp electrophysiology assay. The massively parallel trafficking assay was more accurate (AUC of 0.94) than bioinformatic prediction tools (REVEL and CardioBoost, AUC of 0.81) in discriminating between functionally normal and abnormal variants. Interestingly, over half of variants in exon 2 were found to be functionally normal, suggesting a nuanced interpretation of variants in this 'hotspot' domain is necessary. Our massively parallel trafficking assay can provide this information prospectively.</span></p>
[3/3] Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation
<p>Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation</p>
[2/3] Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation
<p>Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation</p>
[1/3] Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation
<p>Patch clamp of a Purkinje cell in a simulated cerebellar cortex model during single impulse stimulation</p>
Patch clamp dataset for: A massively parallel assay accurately discriminates between functionally normal and abnormal variants in a hotspot domain of KCNH2
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Patch-clamp dataset (SyncroPatch384PE) for control variants used in a calibrated functional patch clamp assay to enhance clinical variant interpretation in KCNH2-related long QT syndrome
<p><span>Modern sequencing technologies have revolutionised our detection of gene variants. However, in most genes, including <em>KCNH2</em>, the majority of missense variants are currently classified as variants of uncertain significance (VUS). The aim of this study </span><span class="normaltextrun"><span>was to investigate the utility of an automated patch-clamp assay for aiding clinical variant classification in the <em>KCNH2 </em>gene. </span></span><span>The assay was designed according to recommendations proposed by the ClinGen Sequence Variant Interpretation Working Group. Thirty-one variants (17 pathogenic/likely pathogenic, 14 benign/likely benign) were classified internally as variant controls. They were heterozygously expressed in Flp-In HEK293 cells for assessing the effects of variant on current density and channel gating in order to determine the sensitive and specificity of the assay. All 17 pathogenic variant controls had reduced current density and 13/14 benign variant controls had normal current density, which enabled determination of normal and abnormal ranges for applying moderate or supporting evidence strength for VUS reclassification. Inclusion of <em>KCNH2</em> functional assay evidence enabled us to reclassify 6 out of 44 VUS as likely pathogenic. The high-throughput patch clamp assay can provide moderate strength evidence for clinical interpretation of clinical <em>KCNH2</em> variants and </span><span>demonstrates the value of developing automated patch clamp assays for functional characterisation of ion channel gene variants.</span></p>
Patch clamp recording from rat hippocampal neuron culture using bulk and NEA chip
<p>Patch-clamp, in current-clamp mode, recordings from rat hippocampal neuron culture using stimulated nano electrode array chip in bulk and low-density NEA chip. All the uncompressed data are in easy-to-use .mat files divided for type of chip (Bulk and NEA); type of coating material and type of stimulation (only for Bulk).</p>
Patch-clamp dataset (SyncroPatch384PE) for control variants used in a calibrated functional patch clamp assay to enhance clinical variant interpretation in KCNH2-related long QT syndrome
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patch clamp
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Correlating anatomy and function with gene expression in individual neurons by combining in vivo labeling, patch clamp and single cell RNA-seq
GEO Series GSE90822. Mus musculus. 927 samples. Type: Expression profiling by high throughput sequencing.
Robust RNA-Seq of aRNA-amplified single cell material collected by patch clamp
GEO Series GSE144216. Homo sapiens. 73 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA-seq of astrocytes and neurons from primary culture of the mouse brain and of acute slice of interneurons from Patch-clamp pipette [RNA-seq mouse brain]
GEO Series GSE231725. Mus musculus. 28 samples. Type: Expression profiling by high throughput sequencing.
Single-cell RNA sequencing of aspirates from cortical neurons after patch clamp recording
GEO Series GSE70844. Mus musculus. 83 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.