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Dataset results
13 results for “phototransduction”
Phototransduction in a marine sponge provides insights into the origin of animal vision: CEL-Seq2 analyses
<p>Most cellular organisms respond to light. In animals, the phototransduction system has evolved from localised light-detecting photoreceptors to ultimately complex eyes and vision. Here, we investigate the origin of metazoan photodetection and transduction by comparing well-characterised opsin-based photosystems in neural animals with those in the sponge <em>Amphimedon queenslandica</em>. Although sponges lack neurons and opsin genes, they can respond rapidly to light. In <em>Amphimedon </em>larva, this is guided by the light-sensing posterior pigment ring. We first use cell type-specific transcriptomes to reveal that genes that characterise eumetazoan Gt- and Go-mediated photosystems are enriched in the <em>Amphimedon </em>pigment ring. We then apply a suite of cell signalling pathway agonists and antagonists to swimming larvae exposed to directional light. These experiments implicate metabotropic glutamate receptors, phospholipase-C, protein kinase C and voltage-gated calcium channel in larval phototaxis. We find that U-73122-inhibition of phospholipase-C, a key transducer of the Gq-mediated pathway, completely reverses phototactic behaviour. Together, these results are consistent with aneural sponges sharing with neural metazoans an ancestral set of signal transduction pathways that have been maintained in these disparate photosensory systems since the last common animal ancestor.</p>
Photopic flicker optoretinography captures the light-driven length modulation of photoreceptors during phototransduction
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Phototransduction in a marine sponge provides insights into the origin of animal vision: CEL-Seq2 analyses
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Molecular evolution of phototransduction pathway genes in nocturnal and diurnal fireflies (Coleoptera: Lampyridae)
<p>Most organisms are dependent on sensory cues from their environment for survival and reproduction. Fireflies (Coleoptera: Lampyridae) represent an ideal system for studying sensory niche adaptation due to many species relying on bioluminescent communication, as well as a diversity of ecology. Here we examine the phototransduction pathway in this non-model organism, and provide some of the first evidence for positive selection in the PT pathway in insects. Duplications are found in calmodulin, inactivation no afterpotential C, inactivation no afterpotential D, and transient receptor potential. We also find strong support for positive selection in arrestin-2, inactivation no afterpotential D, and transient receptor potential, with weak support for positive selection in guanine nucleotide-binding protein G(q) subunit alpha and neither inactivation nor afterpotential C. This represents an exciting new avenue of study as we seek to further understand these molecular players.</p>
Data from: Multi-scale, numerical modeling of spatio-temporal signaling in cone phototransduction
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Molecular evolution of phototransduction pathway genes in nocturnal and diurnal fireflies (Coleoptera: Lampyridae)
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Data from: Shifts in selective pressures on snake phototransduction genes associated with photoreceptor transmutation and dim-light ancestry
The visual systems of snakes are heavily modified relative to other squamates, a condition often thought to reflect their fossorial origins. Further modifications are seen in caenophidian snakes, where evolutionary transitions between rod and cone photoreceptors, termed photoreceptor transmutations, have occurred in many lineages. Little previous work, however, has focused on the molecular evolutionary underpinnings of these morphological changes. To address this, we sequenced seven snake eye transcriptomes and utilized new whole genome and targeted capture sequencing data. We used this data to analyze gene loss and shifts in selection pressures in phototransduction genes that may be associated with snake evolutionary origins and photoreceptor transmutation. We identified the surprising loss of rhodopsin kinase (GRK1), despite a low degree of gene loss overall and a lack of relaxed selection early during snake evolution. These results provide some of the first evolutionary genomic corroboration for a dim-light ancestor that lacks strong fossorial adaptations. Our results also indicate that snakes with photoreceptor transmutation experienced significantly different selection pressures from other reptiles. Significant positive selection was found primarily in cone-specific genes, but not rod-specific genes, contrary to our expectations. These results reveal potential molecular adaptations associated with photoreceptor transmutation, and also highlight unappreciated functional differences between rod- and cone-specific phototransduction proteins. This intriguing example of snake visual system evolution illustrates how the underlying molecular components of a complex system can be reshaped in response to changing selection pressures.
Data from: Implications of dimeric activation of PDE6 for rod phototransduction
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Data from: Shifts in selective pressures on snake phototransduction genes associated with photoreceptor transmutation and dim-light ancestry
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Reactivating the phototransduction cascade with a mutation agnostic gene therapy preserves vision in Rod-Cone Dystrophies
GEO Series GSE286529. Mus musculus. 5 samples. Type: Expression profiling by high throughput sequencing.
Evolutionary conservation of phototransduction and clock gene expression in the cave beetle Ptomaphagus hirtus (Coleoptera, Leiodidae)
GEO Series GSE28039. Ptomaphagus hirtus. 1 samples. Type: Expression profiling by high throughput sequencing.
Gene profiling studies in postnatal Mfrprd6 mutant eyes reveal differential expression of Prss56, a trypsin-like serine protease, and genes involved in visual and phototransduction pathways.
GEO Series GSE53411. Mus musculus. 12 samples. Type: Expression profiling by array.
Remarkable composition shift of cone subtypes combined with changes on phototransduction due to cone mosaicism induced by ARR3 variants
GEO Series GSE309092. Rattus. 8 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.