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13 results for “plant propagation”
plant propagation method: plant-propagation-method
plant propagation method for all available taxa
Fig. 2 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
Fig. 2. The effect of MS medium salt concentrations, sucrose and PG on the germination of somatic embryos of L. montana. The results are expressed as the means with SE from five replicates per treatment. The data were recorded after 8 weeks of culture. Means ± SE followed by the same letter are not significantly different at the 5% level as determined by Duncan's multiple range test.
Fig. 4 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
Fig. 4. TEM analyses of different developmental stages of somatic embryos of L. montana. Formation of cell with large vacuole (V), mitochondria (M), nucleus (N) and nucleolus (NU) in globular embryos (Bar, 0.5 μm) (A). More vacuolated with cytoplasmic organelles in SM regions of pear-shaped (Bar, 0.5 μm) (B) and early torpedo-shaped (Bar, 0.5 μm) (C) embryos. Development of cytoplasmic components with nucleus (N), nucleolus (NU), Golgi apparatuses (GA), mitochondria (M) and chloroplasts (CP) in SM regions of torpedo (Bar, 0.5 μm and 0.2 μm) (D) and cotyledonary embryos (Bar, 0.5 μm) (E). Large intercellular spaces (IS) and thick cell wall (CW) in connective regions of SM and RM of cotyledonary embryos (Bar, 0.5 μm and 0.2 μm) (F). Development of thick IS and CW with cytoplasmic components of mitochondria, starch grains (SG) and plastids (PS) in RMs of torpedo (Bar, 0.2 μm) (G) and cotyledonary (Bar, 0.5 μm and 0.2 μm) (H) embryos.
Fig. 1 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
Fig. 1. In vitro plant regeneration from somatic embryos in cell suspension culture of L. montana: A. Production of FEC on solid MS medium plus 35 g L −1, 10 μM 2,4-D and 2 μM TDZ. B. Formation of white and soft embryo stages in MSL after 3 weeks of culture. C. Different developmental stages of somatic embryos on MSL medium plus 1 μM 2,4-D and 0.5 μM TDZ after 6 weeks of culture. D. Proliferation and germination of embryos on MSL containing 0.5 μM 2,4-D and 1 μM TDZ. E. Germination of SEs on MSL plus 1 μM 2,4-D and 2 μM TDZ. F. Germination of clustered SEs in solid MS medium containing 15 g L −1 sucrose and 10 μM PG. G. Germinated SEs on plant induction medium. H and I. Well-developed plantlets from plant induction medium. J. Acclimatized plants of L. montana in the greenhouse after 3 months.
The origin and genetic variability of vegetatively propagated clones identified from old planted trees and plantations of Thujopsis dolabrata var. hondae in Ishikawa Prefecture, Japan
<p class="Keywords"><span><a name="_Hlk10084612">Clonal plantations of <i>Thujopsis dolabrata</i> var. <i>hondae </i>have been established in Ishikawa Prefecture, Japan, since at least the 1800s. Historical planting of the species has led to the development of vegetatively propagated local cultivars, which originated from 'donor' trees that have often been conserved in sacred groves or avenues at shrines and temples. These donor trees must have been selected from natural populations. In this study we estimated the origin and genetic variability of clones identified among old planted trees and clonal plantations of<i> T. dolabrata </i>var. <i>hondae</i>, using 19 microsatellite markers. We discovered 12 clones among old planted trees, including five identical to members of a set of 14 we previously identified in plantations (giving 21 clones in total). Based on analyses combining assignment and exclusion tests, we inferred origins of eight of those 21 clones: six may have originated from a natural population distributed in Ishikawa, one from Hokkaido & Aomori, and the other from Iwate & Yamagata, suggesting the clones constituting cultivars have multiple origins. The clones identified in plantations have significantly lower genetic variability, and higher relatedness, indicating that clones of cultivars have a much narrower genetic base than those of natural populations. We suggest new clones selected from natural populations</a> elsewhere, as well as Ishikawa, are needed for future breeding of <i>T. dolabrata</i> var. <i>hondae</i> to develop clonal forestry for this species.</span></p>
Data from: Using sensitivity analysis to identify key factors for the propagation of a plant epidemic
Identifying the key factors underlying the spread of a disease is an essential but challenging prerequisite to design management strategies. To tackle this issue, we propose an approach based on sensitivity analyses of a spatiotemporal stochastic model simulating the spread of a plant epidemic. This work is motivated by the spread of sharka, caused by Plum pox virus, in a real landscape. We first carried out a broad-range sensitivity analysis, ignoring any prior information on six epidemiological parameters, to assess their intrinsic influence on model behaviour. A second analysis benefited from the available knowledge on sharka epidemiology and was thus restricted to more realistic values. The broad-range analysis revealed that the mean duration of the latent period is the most influential parameter of the model, whereas the sharka-specific analysis uncovered the strong impact of the connectivity of the first infected orchard. In addition to demonstrating the interest of sensitivity analyses for a stochastic model, this study highlights the impact of variation ranges of target parameters on the outcome of a sensitivity analysis. With regard to sharka management, our results suggest that sharka surveillance may benefit from paying closer attention to highly-connected patches whose infection could trigger serious epidemics.
Table 2 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
<p><b>Table 2</b> Enhancement of somatic embryos and germination frequency in cell suspension culture from friable embryogenic callus (FEC) of L. montana.</p><table><tbody><tr><th>Sucrose (g L <i>−</i> 1) + PGR (μM) in solid MS medium</th><th>PGR (μM) in liquid MS medium</th><th>Liquid MS medium (MSL)</th><th>Number of SEs/SCV developmental stage</th><th>Germination (%)</th></tr></tbody><tbody><tr><th>6 weeks of culture</th><td>4 weeks of culture</td><td>2 weeks of culture</td><td>Globular</td><td>Different stages of embryo*</td><td></td></tr><tr><th>35 + 10 picloram + 2 BA</th><td>1 picloram</td><td>MSL</td><td>10.0 e</td><td>6.4 e</td><td>0</td></tr><tr><td>1 2,4-D</td><td>MSL</td><td>14.4 cd</td><td>9.0 cd</td><td>0</td></tr><tr><td>1 2,4-D + 0.5 BA</td><td>MSL</td><td>15.0 cd</td><td>9.8 cd</td><td>7.2 e</td></tr><tr><td>1 2,4-D + 0.5 <i>m</i> TR</td><td>MSL</td><td>7.8 f</td><td>6.0 e</td><td>8.6 de</td></tr><tr><td>1 2,4-D + 0.5 TDZ</td><td>MSL</td><td>18.0 b</td><td>12.4 b</td><td>14.0 ab</td></tr><tr><th>35 + 10 2,4-D + 2 TDZ</th><td>1 picloram</td><td>MSL</td><td>6.8 fg</td><td>3.0 f</td><td>0</td></tr><tr><td>1 2,4-D</td><td>MSL</td><td>16.0 cd</td><td>6.8 e</td><td>0</td></tr><tr><td>1 2,4-D + 0.5 BA</td><td>MSL</td><td>18.4 b</td><td>10.0 c</td><td>11.4 c</td></tr><tr><td>1 2,4-D + 0.5 <i>m</i> TR</td><td>MSL</td><td>10.2 e</td><td>6.8 e</td><td>14.3 ab</td></tr><tr><td>1 2,4-D + 0.5 TDZ</td><td>MSL</td><td>21.4 a</td><td>14.6 a</td><td>15.2 a</td></tr></tbody></table><p>PGR = Plant growth regulator. <sub>MSL</sub> = Liquid MS medium.SEs = Somatic embryos.SCV = Settled cell volume.*Different stages of embryo = pear-shaped, early torpedo-shaped, torpedoshaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate. Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.</p>
Table 1 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
<p><b>Table 1</b> Effect of sucrose and growth regulators on friable embryogenic callus (FEC) production for somatic embryo (SE) development with 6 weeks of incubation in cell suspension culture of L. montana.</p><table><tbody><tr><th>Sucrose (g L <i>−</i> 1) + PGR (μM) in solid MS medium</th><th>Number of SEs/SCV developmental stage</th><th>Germination (%)</th></tr></tbody><tbody><tr><th>6 weeks of culture</th><td>Globular</td><td>Different stages of embryo*</td><td></td></tr><tr><th>Control</th><td>0</td><td>0</td><td>0</td></tr><tr><th>30 + 10 2,4-D</th><td>4.0 ef</td><td>3.0 gh</td><td>0</td></tr><tr><th>35 + 10 2,4-D</th><td>6.2 de</td><td>4.6 ef</td><td>0</td></tr><tr><th>40 + 10 2,4-D</th><td>7.8 cd</td><td>6.4 de</td><td>0</td></tr><tr><th>35 + 10 2,4-D + 2 BA</th><td>9.4 c</td><td>8.0 c</td><td>0</td></tr><tr><th>35 + 10 2,4-D + 2 TDZ</th><td>14.8 ab</td><td>9.8 a</td><td>0</td></tr><tr><th>30 + 10 picloram</th><td>3.6 f</td><td>2.0 h</td><td>0</td></tr><tr><th>35 + 10 picloram</th><td>7.2 cd</td><td>5.0 ef</td><td>0</td></tr><tr><th>40 + 10 picloram</th><td>10.4 c</td><td>6.6 de</td><td>0</td></tr><tr><th>35 + 10 picloram + 2 BA</th><td>16.2 a</td><td>9.2 ab</td><td>0</td></tr><tr><th>35 + 10 picloram + 2 TDZ</th><td>9.8 c</td><td>7.0 cd</td><td>0</td></tr></tbody></table><p>FEC = Friable embryogenic callus. PGR = Plant growth regulator.SEs = Somatic embryos. SCV = Settled cell volume. *Different stages of embryo = pear-shaped, early torpedoshaped, torpedo-shaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate. Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.</p>
Data from: Using sensitivity analysis to identify key factors for the propagation of a plant epidemic
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The origin and genetic variability of vegetatively propagated clones identified from old planted trees and plantations of Thujopsis dolabrata var. hondae in Ishikawa Prefecture, Japan
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Data from: An individual-based model of seed and rhizome propagated perennial plant species and sustainable management of Sorghum halepense in soybean production systems in Argentina
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Fig. 3 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
Fig. 3. Histological (LS) analyses of different developmental stages of somatic embryos of L. montana. Globular embryo containing meristematic cellular ovate regions (MCO) with smaller to larger isodiametric cells, intense cell division with two celled (TC) and four celled (FC) with vacuolated and intercellular space (IS) (A). The bipolar structure with both shoot (SM) and root (RM) meristems of pear-shaped (B), early torpedo-shaped (C), torpedo-shaped (D) and cotyledonary stage (E) embryos. Epidermis (EP), procambial strands (PS) and vascular tissues (VT) in SM regions of early-torpedo (F) and cotyledonary stage (G) embryos.The torpedo-shaped embryo showing RM with xylem-like pattern and root cap (RC) (H). Cotyledonary stage embryo showing RM with root hair (RH), epidermis, cortex (C) and vascular cylinder (VC) in RM regions (I). Bar (A) 500 μm, (B–E) 500 μm and (F–I) 100 μm.
Table 3 in Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana
<p><b>Table 3</b> Enhancement of somatic embryos (SEs) and germination frequency in cell suspension culture from friable embryogenic callus (FEC) of L. montana.</p><table><tbody><tr><th>Sucrose (g L <i>−</i> 1) + PGR (μM) in solid MS medium</th><th>PGR (μM) in liquid MS medium</th><th>Liquid MS medium (MSL)</th><th>Number of SEs/SCV developmental stage</th><th>Germination (%)</th></tr></tbody><tbody><tr><th>6 weeks of culture</th><td>4 weeks of culture</td><td>2 weeks of culture</td><td>Globular</td><td>Different stages of embryo*</td><td></td></tr><tr><th>35 + 10 2,4-D + 2 TDZ</th><td>0.5 2,4-D + 1 BA</td><td>MSL</td><td>17.6 b</td><td>9.4 c</td><td>23.0 de</td></tr><tr><td>0.5 2,4-D + 1 <i>m</i> TR</td><td>MSL</td><td>13.2 cd</td><td>8.0 cd</td><td>22.6 e</td></tr><tr><td>0.5 2,4-D + 1 TDZ</td><td>MSL</td><td>26.0 a</td><td>19.4 a</td><td>24.2 d</td></tr><tr><td>1 2,4-D + 1 BA</td><td>MSL</td><td>10.6 e</td><td>7.4 de</td><td>24.4 d</td></tr><tr><td>1 2,4-D + 1 <i>m</i> TR</td><td>MSL</td><td>9.8 ef</td><td>6.2 e</td><td>23.7 de</td></tr><tr><td>1 2,4-D + 1 TDZ</td><td>MSL</td><td>15.4 c</td><td>9.6 c</td><td>32.0 b</td></tr><tr><td>1 2,4-D + 2 BA</td><td>MSL</td><td>7.0 gh</td><td>6.6 e</td><td>22.1 e</td></tr><tr><td>1 2,4-D + 2 <i>m</i> TR</td><td>MSL</td><td>5.6 h</td><td>7.8 de</td><td>26.9 c</td></tr><tr><td>1 2,4-D + 2 TDZ</td><td>MSL</td><td>9.0 ef</td><td>11.8 b</td><td>34.7 a</td></tr></tbody></table><p>PGR = Plant growth regulator. <sub>MSL</sub> = Liquid MS medium. SEs = Somatic embryos. SCV = Settled cell volume. *Different stages of embryo = pear-shaped, early torpedo-shaped, torpedoshaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate.Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.</p>
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