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56 results for “plant-parasitic nematodes”
Fig. 1 in Species Composition And Structure Of The Communities Of Plant-Parasitic And Free-Living Soil Nematodes In The Greenhouses Of Botanical Gardens Of Ukraine
Fig. 1. Dendrogram of similarity of the nematode communities in the greenhouses of botanical gardens of Ukraine (amalgamation by the method of complete linkage). Explanation of the abbreviations is given in table 2. Рис. 1. Дендрограмма сходства нематодных сообществ в оранжереях ботанических садов Украины (объединение по методу полной связи). Расшифровка сокращений дана в таблице 2.
Fig. 2 in Species Composition And Structure Of The Communities Of Plant-Parasitic And Free-Living Soil Nematodes In The Greenhouses Of Botanical Gardens Of Ukraine
Fig. 2. Dendrogram of similarity of plant-parasitic nematodes' communities in the greenhouses of botanical gardens of Ukraine (amalgamation by the method of complete linkage). Explanation of the abbreviations is given in table 2.
Figure 6 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 6: PCR results using Meloidogyne-specific and M. naasi and M. marylandi-specific primers. DL: DNA Ladder; 1: Meloidogyne spp. (DNA ID:9); 2: M. naasi (DNA ID:9); 3: Meloidogyne spp. (DNA ID:4); 4: M. marylandi (DNA ID:4); and 5: Meloidogyne spp.(DNA ID:4).
Figure 3 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 3: PCR results using Hoplolaimus-specific and H. stephanus, H. columbus and H. galeatus-specific primers. DL: DNA Ladder; 1: Hoplolaimus spp. (DNA ID:10); 2: H. stephanus (DNA ID:10); 3: H. columbus (DNA ID:10); 4 H. galeatus (DNA ID:10); 5: Hoplolaimus spp. (DNA ID:3); 6: H. stephanus (DNA ID:3); 7: H. columbus (DNA ID:3); 8 H. galeatus (DNA ID:3); 9: Hoplolaimus spp. (DNA ID:4); 10: H. stephanus (DNA ID:4); 11: H. columbus (DNA ID:4); and 12 H. galeatus (DNA ID:4).
Figure 2 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 2: Phylogeny of the rDNA ITS region of Hoplolaimus spp. isolated from golf putting greens. Phylogenetic trees were constructed with the neighbor-joining algorithm using the Kimura two-parameter model with Litylenchus spp. (LC383724) as the outgroup. Bootstrap values are based on 1000 resamplings of the data set. DNAID codes correlate to Table 2.
Figure 1 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 1: Distribution of plant-parasitic nematode species sampled from creeping bentgrass putting greens in Missouri and eastern Kansas in 2021 and Indiana in 2022 in two independent pie charts. Samples were collected during the months of April, June, August and October of 2021 and 2022, respectively. "n" indicates total PPNs represented within each chart.
Figure 3 in Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review
Figure 3: Hot water dipping tank (A) and the interior of the tank (B) at a commercial nursery in Michigan.
Figure 4 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 4: Scanning-electron micrographs of a lance nematode specimen collected form Site 5. A) four lip annules; B) the presence of an epiptygma; C) 25 longitudinal striae on the basal lip annule; and D) four lateral incisures.
Figure 5 in Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana
Figure 5: Phylogeny of molecularly characterized Meloidogyne spp. isolated from golf coursed based on D2/D3 28S genes. phylogenetic trees were constructed with the neighborjoining algorithm using the Kimura two-parameter model with Litylenchus spp. (LC383724) as the outgroup. Bootstrap values are based on 1000 resamplings of the data set and displayed near branch nodes. DNAID codes correlate to Table 2.
Figure 2 in Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review
Figure 2: Light micrograph of an adult male (A) and head (B) of Aphelenchoides spp. extracted from Heliopsis spp. leaves. Angular lesions (C,D) on the leaves of two varieties of Heliopsis spp. infected with Aphelenchoides spp.
Figure 1 in Plant-Parasitic Nematodes and their Effects on Ornamental Plants: A Review
Figure 1: Light micrograph of Meloidogyne hapla second-stage juvenile (A) extracted from a daylily field at a commercial nursery in Michigan. Daylily roots were taken from the same field showing galling and stunting due to M. hapla infection (B) compared to healthy roots (C).
Linked collectors and determiners for: ARC-PPRI: National Collection of Nematodes and South African Plant-Parasitic Nematode Survey(1901-2014).
Natural history specimen data linked to collectors and determiners held within, "ARC-PPRI: National Collection of Nematodes and South African Plant-Parasitic Nematode Survey(1901-2014)". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="https://bionomia.net/dataset/5edbfe13-c150-43d3-ac38-729b6aeb6147">https://bionomia.net/dataset/5edbfe13-c150-43d3-ac38-729b6aeb6147</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/5edbfe13-c150-43d3-ac38-729b6aeb6147">https://gbif.org/dataset/5edbfe13-c150-43d3-ac38-729b6aeb6147</a>. Formatted as a Frictionless Data package.
Data from: The origin, deployment, and evolution of a plant-parasitic nematode effectorome
<p>Plant-parasitic nematodes constrain global food security. During parasitism, they secrete effectors into the host plant from two types of pharyngeal gland cells. These effectors elicit profound changes in host biology to suppress immunity and establish a unique feeding organ from which the nematode draws nutrition. Despite the importance of effectors in nematode parasitism, there has been no comprehensive identification and characterisation of the effector repertoire of any plant-parasitic nematode.</p> <p>To address this, we advance techniques for gland cell isolation and transcriptional analysis to define a stringent annotation of putative effectors for the cyst nematode <em>Heterodera schachtii </em>at three key life-stages. We define 659 effector gene loci: 293 "known" high-confidence homologs of plant-parasitic nematode effectors, and 366 "novel" effectors with high gland cell expression. In doing so we define a comprehensive "effectorome" of a plant-parasitic nematode.</p> <p>Using this effector definition, we provide the first systems-level understanding of the origin, deployment and evolution of a plant-parasitic nematode effectorome. The robust identification of the comprehensive effector repertoire of a plant-parasitic nematode will underpin our understanding of nematode pathology, and hence, inform strategies for crop protection.</p>
Data from: The origin, deployment, and evolution of a plant-parasitic nematode effectorome
Open the record for dataset details and reuse information.
Towards genetic modification of plant-parasitic nematodes: Delivery of macromolecules to male germlines and expression of exogenous mRNA in second stage juveniles
<p>Plant-parasitic nematodes are a current and future threat to food security, causing an estimated 100 billion USD in crop losses each year. The most problematic are the obligate sedentary endoparasites (primarily root knot nematodes and cyst nematodes). Progress in understanding their biology is held back by a lack of tools for functional genetics. Forward genetics is largely restricted to studies of natural variation in populations, and reverse genetics is entirely reliant on RNA interference. There is an expectation that the development of functional genetic tools would accelerate progress in plant-parasitic nematology, and hence the development of novel control solutions. Here, we develop some of the foundational biology required to deliver a functional genetic "tool kit" in plant-parasitic nematodes. We characterise the gonads of male <em>Heterodera schachtii</em> and Meloidogyne hapla in the context of spermatogenesis. We test and optimise various methods for the delivery, expression, and/or detection of exogenous nucleic acids in plant-parasitic nematodes. We demonstrate that delivery of macromolecules to cyst and root knot nematode male germlines is difficult but possible. Similarly, we demonstrate the delivery of oligonucleotides to root knot nematode gametes. Finally, we develop a transient expression system in plant-parasitic nematodes by demonstrating the delivery and expression of exogenous mRNA encoding various reporter genes throughout the body of <em>H. schachtii</em> juveniles using lipofectamine-based transfection. We anticipate these developments to be independently useful, and, taken together, will expedite the development of genetic modification protocols for sedentary endoparasitic nematodes, and ultimately catalyze research on a group of nematodes that threaten global food security.</p>
FIGURE 7 in Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA
FIGURE 7. Micrographs of Hemicycliophora thienemanni, H. conida, Hemicaloosia graminis from turfgrasses in NC and SC. All scale bars =20µm. A. Female esophageal region of H. thienemanni. B. Vulva region of H. thienemanni. C. Female tail of H. thienemanni. D. Female esophageal region of H. conida. E,F. Vulva region of H. conida. G. Female esophageal region of Hemicaloosia graminis. H. Vulva region of Hemicaloosia graminis. I. Female tail of Hemicaloosia graminis. J. Male esophageal region of Hemicaloosia graminis. K. Male tail of Hemicaloosia graminis.
FIGURE 4 in Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA
FIGURE 4. Micrographs of Belonolaimus longicaudatus and Dolichodorus heterocephalus from turfgrasses in NC and SC. All scale bars=20µm. A. Pharyngeal region of B. longicaudatus. B. Vulva region of B. longicaudatus. C. Female tail of B. longicaudatus. D,E. Male tails of B. longicaudatus. F. Pharyngeal region of D. heterocephalus. G. Vulval region of D. heterocephalus. H. Female tail of D. heterocephalus. I. Male tails of D. heterocephalus.
FIGURE 1 in Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA
FIGURE 1. Micrographs of Pratylenchus penetrans, Tylenchorhynchus claytoni and Filenchus cylindricus from turfgrasses in NC and SC. Scale bars: A, B, J=50 μm; C-I, K-M=20μm. A,B. Entire body of P. penetrans. C. Pharyngeal region of P. penetrans. D,E. Female tails of P. penetrans. F. Male tail of P. penetrans. G. Pharyngeal region of T. claytoni. H. Female tail of T. claytoni. I. Male tail of T. claytoni. J. Entire body of F. cylindricus. K. Pharyngeal region of F. cylindricus. L. Vulval region of F. cylindricus. M. Female tail of F. cylindricus.
FIGURE 6 in Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA
FIGURE 6. Micrographs of Hemicriconemoides chitwoodi, H. wessoni, Paratylenchus goldeni and Aphelenchoides myceliophagus from turfgrasses in NC and SC. Scale bars: A, D=50µm; B, C, E–M=20µm. A. Entire body of H. chitwoodi. B. Pharyngeal region of H. chitwoodi. C. Vulva and tail region of H. chitwoodi. D. Entire body of H. wessoni. E. Pharyngeal region of H. wessoni. F. Vulva and tail region of H. wessoni. G. Pharyngeal region of P. g o l d e n i. H. Vulva and tail region of P. goldeni. I. Pharyngeal region of A. myceliophagus. J. Vulval region of A. myceliophagus. K. Pharyngeal region of A. myceliophagus. L. Female tail of A. myceliophagus. M. Male tail of A. myceliophagus.
FIGURE 3 in Taxonomy and morphology of plant-parasitic nematodes associated with turfgrasses in North and South Carolina, USA
FIGURE 3. Micrographs of J2 of Meloidogyne graminis, M. naasi, Heterodera sp. and Cactodera sp. from turfgrasses in NC and SC. Scale bars: A, E, I, L=50µm; B–D, F–H, J, K, M–O=20µm. A. Entire body of M. graminis. B. Pharyngeal region of M. graminis. C,D. Tail of M. graminis. E. Entire body of M. naasi. F. Pharyngeal region of M. naasi. G,H. Tails of M. naasi. I. Entire body of H. sp. J. Pharyngeal region of H. sp. K. Tail of H. sp. L. Entire body of C. sp. M. Pharyngeal region of C. sp. N,O. Tails of C. sp.
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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