Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
29
datasets available to search
ShareScore release 0.9.0
Dataset results
29 results for “pollen collection”
Construction, validation and application of nocturnal pollen transport networks in an agro-ecosystem: datasets collected using light microscopy and DNA metabarcoding
<p>This dataset contains all data required to reproduce the analyses conducted in Macgregor <em>et al. </em>(2018), using the R Notebook archived at doi: <a href="https://dx.doi.org/10.5281/zenodo.1322712">10.5281/zenodo.1322712</a>.</p> <p>Specifically, the dataset contains details of pollen transport detected on two matched samples, each containing 311 moths of 41 species, using two methods: a traditional light microscopy approach and a novel DNA metabarcoding approach. Both raw and manually-curated versions of each dataset are archived for full clarity. The dataset additionally contains all metadata required to fully interpret these data, including the RGB tables used to prepare Fig 4 in Macgregor <em>et al. </em>(2018).</p> <p>Macgregor <em>et al. </em>(2018) Construction, validation and application of nocturnal pollen transport networks in an agro-ecosystem: a comparison using light microscopy and DNA metabarcoding. <em>Ecological Entomology</em>, doi: <a href="https://dx.doi.org/10.1111/een.12674">10.1111/een.12674</a>.</p>
Pollen collection by the western honey bee and common eastern bumble bee foraging in a common landscape and applications for agri-environment schemes
Open the record for dataset details and reuse information.
Data from: Introduced bees (Osmia cornifrons) collect pollen from both coevolved and novel host-plant species within their family-level phylogenetic preferences
<p><span><span><span><span><span><span><span><span><span><span><span>Studying the pollen preferences of introduced bees allows us to investigate how species utilize host-plants when establishing in new environments. <i>Osmia cornifrons</i> is a solitary bee introduced into North America from East-Asia for pollination of crops in the Rosaceae. We investigated whether <i>O. cornifrons</i> 1) more frequently collected pollen from host-plant species they coevolved with from their geographic origin, or 2) prefer hosts-plant species of specific plant taxa independent of origin. To address this question, using pollen metabarcoding we examined the identity and relative abundance of pollen in larval provisions from nests located in different habitats with varying abundance of East-Asian and non-Asian plant species. Our results show that <i>O. cornifrons</i> disproportionately, yet not exclusively, collected pollen from their native range. Plants in the family Rosaceae were their most preferred pollen hosts, where they differentially collected species native to East-Asia, Europe, or North America depending on the landscape. Our results suggest that while <i>O. cornifrons</i> more frequently collect pollen of East-Asian origin, the collection of pollen from novel species within their phylogenetic familial affinities can facilitate pollinator establishment. This phylogenetic preference highlights the effectiveness of <i>O. cornifrons</i> as crop pollinators of a variety of Rosaceae crops from different geographic origins. Ourresults imply that globalization of non-native plant species may ease the naturalization of their coevolved pollinators outside of their native range. </span></span></span></span></span></span></span></span></span></span></span></p>
Figure 2. - Nesting architecture of Xylocopanasalis; Dissected nests of Xylocopanasalis revealing the nest structure inside the bamboo culm and its residents. Measurements of the nest parameters are shown in Table 1. The diameters of the nests (excluding the nest thickness) were measured at the nest entrance, followed by the vestibulum (antechamber) length, cell length, and the inner most cell length, respectively (2a). Cells containing larvae with pollen masses and their feces were collected and weighted (2b).
Figure 2. - Nesting architecture of Xylocopanasalis; Dissected nests of Xylocopanasalis revealing the nest structure inside the bamboo culm and its residents. Measurements of the nest parameters are shown in Table 1. The diameters of the nests (excluding the nest thickness) were measured at the nest entrance, followed by the vestibulum (antechamber) length, cell length, and the inner most cell length, respectively (2a). Cells containing larvae with pollen masses and their feces were collected and weighted (2b).
Figure 3. - Pollen grains collected by Xylocopanasalis; Some representations of pollens collected from pollen masses of Xylocopanasalis. The "major" pollen sources: Fagaceae, Castanopsis sp. (3a); Elaeagnaceae, Elaeagnuscf.latifolia (3b); Fabaceae, Cassia sp. (3c and 3d), Sennasiamea (3e and 3f); Acanthaceae, Thunbergia (3g and 3h).
Figure 3. - Pollen grains collected by Xylocopanasalis; Some representations of pollens collected from pollen masses of Xylocopanasalis. The "major" pollen sources: Fagaceae, Castanopsis sp. (3a); Elaeagnaceae, Elaeagnuscf.latifolia (3b); Fabaceae, Cassia sp. (3c and 3d), Sennasiamea (3e and 3f); Acanthaceae, Thunbergia (3g and 3h).
Data from: Introduced bees (Osmia cornifrons) collect pollen from both coevolved and novel host-plant species within their family-level phylogenetic preferences
Open the record for dataset details and reuse information.
Learning to handle flowers increases pollen collection benefits for bees but does not affect pollination success for plants
Open the record for dataset details and reuse information.
Data from: Application of ITS2 metabarcoding to determine the provenance of pollen collected by honey bees in an agroecosystem
Premise of the study: Melissopalynology, the identification of bee-collected pollen, provides insight into the flowers exploited by foraging bees. Information provided by melissopalynology could guide floral enrichment efforts aimed at supporting pollinators, but it has rarely been used because traditional methods of pollen identification are laborious and require expert knowledge. We approach melissopalynology in a novel way, employing a molecular method to study the pollen foraging of honey bees (Apis mellifera) in a landscape dominated by field crops, and compare these results to those obtained by microscopic melissopalynology. Methods: Pollen was collected from honey bee colonies in Madison County, Ohio, USA, during a two-week period in mid-spring and identified using microscopic methods and ITS2 metabarcoding. Results: Metabarcoding identified 19 plant families and exhibited sensitivity for identifying the taxa present in large and diverse pollen samples relative to microscopy, which identified eight families. The bulk of pollen collected by honey bees was from trees (Sapindaceae, Oleaceae, and Rosaceae), although dandelion (Taraxacum officinale) and mustard (Brassicaceae) pollen were also abundant. Discussion: For quantitative analysis of pollen, using both metabarcoding and microscopic identification is superior to either individual method. For qualitative analysis, ITS2 metabarcoding is superior, providing heightened sensitivity and genus-level resolution.
FIGURE 4. Pollen and achenes. A in A taxonomic revision of the genus Antiphiona (Inuleae, Asteraceae), including a report of the first collection of the genus for South Africa
FIGURE 4. Pollen and achenes. A. Spinose tricolporate pollen of Antiphiona fragrans (Story 5854); B. A. pinnatisecta (Koekemoer 5000); C. Rigid twin hairs on the achenes of A. fragrans (Strey 2410); D. A. pinnatisecta (Schoenfelder 15568). Scale bar = 10 μm (A–B); 200 μm (C); 100 μm (C).
Data from: How a generalist bee achieves high efficiency of pollen collection on diverse floral resources
Bees foraging for floral rewards are one of our most thoroughly studied examples of generalist foraging ecology. Generalist bees rely considerably on instrumental (associative) learning to acquire routines that allow them to collect nectar efficiently from diverse plant species. Although such bees must also collect pollen from diverse species, few studies have examined if and how high efficiency is achieved. We characterized how generalist bumble bees (Bombus impatiens) foraged effectively for pollen from diverse floral resources, by manipulating the presence of pollen and anther cues, in a series of experiments using pollen-bearing live flowers, flowers of a sterile pollenless horticultural hybrid, and artificial flowers. We show that generalist bumble bees exhibit flexible and effective pollen collection by switching between 2 routines: "scrabbling" when pollen is abundant and "sonicating" when pollen is scarce. Efficient switching between these behaviors is regulated by the interplay of 2 ubiquitous floral cues: chemical anther cues stimulating pollen collection behavior and mechanical pollen cues suppressing sonication (and eliciting scrabbling). Flexible pollen collection behavior is functional: When pollen on anthers was scarce, bees collected it at a greater rate by sonicating than scrabbling. This mechanism of behavioral flexibility likely allows generalist bees to handle diverse anther morphologies efficiently and may have facilitated the recurrent evolution of plant species that conceal pollen rewards via pored floral morphology. Whereas effective nectar foraging relies heavily on associative learning of unique routines for each flower type, a weighing of 2 types of cues regulates the flexible pollen collection mechanism we describe.
FIGURES 7–12 in Character analysis of adaptations for tarsal pollen collection in the Bombyliidae (Insecta: Diptera): the benefits of putting your foot in your mouth
FIGURES 7–12. Female tarsal setae of Bombyliidae. 7. Poecilognathus sulphureus, spatulate setae, 2000 x. 8. P. sulphureus, lateral view tarsomeres 3, 4, and 5 with microtrichia, normal setae and apicoventral spatulate setae, 300 x. 9. Hemipenthes jaennickeana, apex foretarsal spatulate setae, 2660 x. 10. Exoprosopa eremita, distal tarsomeres and pretarsus with weakly spatulate setae, 270 x. 11. H. jaennickeana, foretarsal spatulate setae plus microtrichia, 990 x. 12. P. lucifer, lateral view of foretarsomere 2 with elongate, weakly clavate setae, 160 x.
FIGURES 2–6 in Character analysis of adaptations for tarsal pollen collection in the Bombyliidae (Insecta: Diptera): the benefits of putting your foot in your mouth
FIGURES 2–6. Female tarsal setae of Bombyliidae. 2. Neodiplocampta paradoxa, foretarsus with capitate setae, 231 x. 3. Dipalta serpentina, apex of foretarsal capitate seta, 800 x. 4. Dipalta serpentina, foretarsal capitate setae plus microtrichia, 800 x. 5. Neodiplocampta paradoxa, foretarsus plus apex of tibia, 63 x. 6. Bombylius major, tarsomere 5 and pretarsus of foreleg with lanceolate setae, 370 x.
FIGURE 1 in Character analysis of adaptations for tarsal pollen collection in the Bombyliidae (Insecta: Diptera): the benefits of putting your foot in your mouth
FIGURE 1. Female of Hemipenthes jaennickeana on staminatephase flower of Sabatia campestris. A. Right foretarsi stroking dehiscing anther. B. Right foreleg transferring pollen to labellum.
Figure 2 in Differentiated use of pollen sources by two sympatric species of oil-collecting bees (Hymenoptera: Apidae)
Figure 2. Pollen types used by Centris analis and Centris tarsata in larval provision. (A) Heteropterys sp. (polar view); (B) Solanum lycocarpum (polar view); (C) Banisteriopsis malifolia (polar view); (D) Byrsonima sp. (equatorial view); (E) Senna sp1 (equatorial view) and (F) Machaerium sp. (equatorial view).
Individual bee foragers are less efficient transporters of pollen for the plants from which they collect the most pollen into their scopae
<p><strong>PREMISE</strong>: Bees provision most of the pollen they remove from anthers to their larvae and transport only a small proportion to stigmas, which can negatively affect plant fitness. Though most bee species collect pollen from multiple plant species, we know little about how the efficiency of bees' pollen transport varies among host plant species, or how it relates to other aspects of generalist bee foraging behavior that benefit plant fitness, such as specialization on individual foraging bouts.</p> <p><strong>METHODS</strong>: We compared the pollen collected and transported by three bee species for 46 co-occurring plant species. Specifically, we compared the relative abundance of pollen taxa in individual bees' scopae, structures where bees store pollen to provision larvae, with the relative abundance of pollen taxa on the rest of bees' bodies, which is more likely to be transferred to stigmas. </p> <p><strong>RESULTS</strong>: Bees carried five times more pollen grains in their scopae than elsewhere on their bodies. Within foraging bouts, bees were relatively specialized in their pollen collection, but transported proportionally less pollen for the host plants on which they specialized. Across foraging bouts, two bee species transported proportionally less pollen for some of their host plants than for others, though differences didn't consistently follow the same trend as at the foraging bout scale.</p> <p><strong>CONCLUSIONS</strong>: Our results suggest that foraging bout specialization, which is known to reduce heterospecific pollen transfer, also results in less efficient pollen transport. Thus, bee foragers that visit predominantly one plant species may have contrasting effects on that plant's fitness. </p>
Individual bee foragers are less efficient transporters of pollen for the plants from which they collect the most pollen into their scopae
Open the record for dataset details and reuse information.
Global patterns in bumble bee pollen collection show phylogenetic conservation of diet
Open the record for dataset details and reuse information.
Data from: Application of ITS2 metabarcoding to determine the provenance of pollen collected by honey bees in an agroecosystem
Open the record for dataset details and reuse information.
Data from: Honey bee dietary neonicotinoid exposure is associated with pollen collection from agricultural weeds
Open the record for dataset details and reuse information.
Data from: How a generalist bee achieves high efficiency of pollen collection on diverse floral resources
Open the record for dataset details and reuse information.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.