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79 results for “ribosomal DNA”
Fig. 3 in Relationships Of The Heteronchocleidids (Heteronchocleidus, Eutrianchoratus And Trianchoratus) As Inferred From Ribosomal Dna Nucleotide Sequence Data
Fig. 3. Bayesian consensus tree for the anabantoids, channids and catfishes (silurids, bagrids, clariids) obtained using partial Cytochrome b sequences with cyprinids as outgroup. The heteronchocleidids genera present on the anabantoids and channids are shown with their geographical areas. Values shown at each node refer to Bayesian posterior probabilities. (*refer to Table 3 for names used in GenBank).
Fig. 2. Bayesian consensus tree generated from partial 28S in Relationships Of The Heteronchocleidids (Heteronchocleidus, Eutrianchoratus And Trianchoratus) As Inferred From Ribosomal Dna Nucleotide Sequence Data
Fig. 2. Bayesian consensus tree generated from partial 28S rDNA sequences (D1 domain) with Diplectanum spp. and Gyrodactylus spp. as outgroups. Values shown at each node refer to Bayesian (BI) posterior probabilities/maximum likelihood (ML) percentages of the bootstrap values with 100 replicates. Bootstrap values lower than 50 are given as dashes (-).
Fig. 1 in Relationships Of The Heteronchocleidids (Heteronchocleidus, Eutrianchoratus And Trianchoratus) As Inferred From Ribosomal Dna Nucleotide Sequence Data
Fig. 1. Neighbour joining (NJ) tree constructed by PAUP* using partial 28S rDNA sequences (D1 domain) with Diplectanum spp. and Gyrodactylus spp. as outgroups. Percentages of the bootstrap values for neighbour joining (NJ)/maximum parsimony (MP) (NJ & MP=1,000 replicates) are shown along the branches. Bootstrap values lower than 50 are given as dashes (-).
Fig. 3 in Broad Taxon Sampling of Ciliates Using Mitochondrial Small Subunit Ribosomal DNA
Fig. 3. Concatenated mitochondrial and nuclear SSU-rDNA tree inferred from an alignment of 2333 included characters. Most likely ML tree is shown; the BI tree was the same for well-supported nodes. Node support is as in Fig 1.
Fig. 2 in Broad Taxon Sampling of Ciliates Using Mitochondrial Small Subunit Ribosomal DNA
Fig. 2. Nuclear SSU-rDNA tree inferred from an alignment of 1543 included characters. The most likely ML tree is shown; the BI tree was the same for well-supported nodes. Node support is as in Fig 1.
Fig. 1 in Broad Taxon Sampling of Ciliates Using Mitochondrial Small Subunit Ribosomal DNA
Fig. 1. Mitochondrial SSU-rDNA tree inferred from an alignment of 790 included characters. The most likely ML tree is shown; the BI tree was the same for well-supported nodes. Node support is shown as: ML bootstraps/BI posterior probability. Values ≤ 50 are shown as "-".
Fig. 3. Maximum likelihood tree constructed from 38 nuclear rDNA ITS1 and ITS2 sequences from Apiaceae genus Daucus and relatives using a in Molecular phylogeny of Daucus (Apiaceae): Evidence from nuclear ribosomal DNA ITS sequences
Fig. 3. Maximum likelihood tree constructed from 38 nuclear rDNA ITS1 and ITS2 sequences from Apiaceae genus Daucus and relatives using a transition/transversion rate ratio of 1.6. Branch lengths are proportional to the number of expected nucleotide substitutions per site.
Fig. 2 in Molecular phylogeny of Daucus (Apiaceae): Evidence from nuclear ribosomal DNA ITS sequences
Fig. 2. Neighbor-joining tree inferred form the analysis of 39 nuclear rDNA ITS1 and ITS2 sequences from Apiaceae genus Daucus and its relatives using a transition/transversion rate ratio of 1.6. Branch lengths are proportional to distance estimated from the two parameter method of Kimura. Numbers at nodes indicate bootstrap values for 100 replicate analyses. On this tree, bootstrap values <20% are not indicated.
Fig. 1 in Molecular phylogeny of Daucus (Apiaceae): Evidence from nuclear ribosomal DNA ITS sequences
Fig. 1. Strict consensus of 11 parsimony trees derived from equally-weighted parsimony analysis of combined nuclear DNA ITS1 and ITS2 sequences from Daucus and its relatives using all unambiguously-aligned positions (CIs with and without uninformative characters= 0.6613 and 0.5817; RI=0.8387). From the left to the right, names of taxa, sections, and clades are given. Numbers above the nodes indicate the number of times a monophyletic group occurred in 100 bootstrap replicates; AutoDecay values are given below.
Figure 3 in Diceratocephala boschmai (Platyhelminthes: Temnocephalida) from crayfish farms in Thailand: investigation of the topographic surface and analysis of 18S ribosomal DNA sequences
Figure 3. The neighbor-joining phylogenetic tree based on the 18S rDNA gene, showing the relationships of D. boschmai with 29 other turbellarian species.
Figure 2 in Diceratocephala boschmai (Platyhelminthes: Temnocephalida) from crayfish farms in Thailand: investigation of the topographic surface and analysis of 18S ribosomal DNA sequences
Figure 2. Surface topography of D. boschmai. A–C) Unhatched and hatched eggs; D) ventral view of a specimen; E) mouth with protruding pharynx; F) thread-like filaments adhered to the pharynx; G, H) a higher magnification of write-dot boxes in 2E; I, J) a higher magnification of write-dot boxes in 2D; K) ventral view of a specimen at posterior end; L) dorsal view of a specimen; M) a higher magnification of write-dot box in Figure L. ad, adhesive disc; af, adhered filaments; ci, ciliated cell; ds, double spines; fi, filament; gr, groove; gv, gravel-like units; in, intertentacular flange; mo, mouth; op, opercular plate; pe, peduncle; pi, pit; sp, single spine; tb, trabecular meshwork; tc, tentacle; th, thread-like filaments; tr, trunk; vi, villi..
Figure 1. A in Diceratocephala boschmai (Platyhelminthes: Temnocephalida) from crayfish farms in Thailand: investigation of the topographic surface and analysis of 18S ribosomal DNA sequences
Figure 1. A) C. destructor harboring adult D. boschmai and eggs of flatworm; B) dorsal view of an extending body; C) diagram of organ structures in dorsal view; D) diagram of reproductive complex; E, F) photomicrograph and diagram of penial stylet, respectively; G) unhatched and hatched eggs. ad, adhesive disc; at, atrium; cv, contractile vesicle; ds, dorsal side; es, ejaculatory sac; ey, eye; ev, excretory vesicle; fi, filament; in, intertentacular flange; ine, intestine; int, introvert; mo, mount; ov, ovary; pe, peduncle; pf, plane of fracture; ph, pharynx; pn, subepidermal pigment network; ps, penial stylet; rv, resorbens vesicle; s, stalk; se, seminal vesicle; sr, seminal receptacle; sp, sclerotized papillae; tc, tentacle; te, testis; tg, tentacular gland; ve, vasa efferentia; vg, vagina; vi, vitellaria; vs, ventral side.
The alignments of chloroplast genome sequences and nuclear ribosomal DNA fragments of six oak species sampled in the hot-dry valley of the Jinsha River, southwestern China
<p>Both chloroplast (cp) genome sequences and nuclear ribosomal (nr) DNA were assembled using GetOrganelle v.1.7.6.1 for 18 oak trees sampled in the Panzhihua Cycad National Nature Reserve, Sichuan Province, China. These trees belong to six oak species, including Quercus cocciferoides, Q. dolicholepis, Q. franchetii, Q. griffithii, Q. longispica, and Q. variabilis. We used PhyloSuite v.1.1.152 to extract coding sequences (CDSs), tRNA genes, rRNA genes, introns, and intergenic spacers (IGSs) of the 18 oak cp genomes. These sequences were aligned separately using MAFFT v.7.3.13 and manually adjusted with BioEdit v.7.2.5. Length variations in mononucleotide repeats were excluded and inversions were replaced with their reverse complements because of their tendency for homoplasy. Other indels were coded as binary characters according to the simple gap coding method using GapCoder. Separate assignments were concatenated according to their respective positions in the cp genome to obtain the alignments of LSC, SSC, IRb, and the whole cp genome.</p>
Capturing single-copy nuclear genes, organellar genomes, and nuclear ribosomal DNA from deep genome skimming data for plant phylogenetics: A case study in Vitaceae
<p>With the decreasing cost and availability of many newly developed bioinformatics pipelines, next-generation sequencing (NGS) has revolutionized plant systematics in recent years. Genome skimming has been widely used to obtain high-copy fractions of the genomes, including plastomes, mitochondrial DNA (mtDNA), and nuclear ribosomal DNA (nrDNA). In this study, through simulations, we evaluated the optimal (minimum) sequencing depth and performance for recovering single-copy nuclear genes (SCNs) from genome skimming data, by subsampling genome resequencing data and generating 10 datasets with different sequencing coverage <i>in silico</i>. We tested the performance of four datasets (plastome, nrDNA, mtDNA, and SCNs) obtained from genome skimming based on phylogenetic analyses of the <i>Vitis</i> clade at the genus level and Vitaceae at the family level, respectively. Our results showed that optimal minimum sequencing depth for high-quality SCNs assembly via genome skimming was about 10× coverage. Without the steps of synthesizing baits and enrichment experiments, coupled with incredibly low sequencing costs, we showcase that deep genome skimming (DGS) is as effective for capturing large datasets of SCNs as the widely used Hyb-Seq approach, in addition to capturing plastomes, mtDNA, and entire nrDNA repeats. DGS may serve as an efficient and economical alternative and may be superior to the popular target enrichment/Hyb-Seq approach.</p>
New insights into infrageneric relationships of Lonicera (Caprifoliaceae) as revealed by nuclear ribosomal DNA cistron data and plastid phylogenomics
<p>The discontinuous geographic distribution pattern of plants in the north temperate zone has been a focus of biogeographic research, especially concerning the mechanisms behind the formation of such a pattern and the spatial and temporal evolution of this intermittent distribution pattern. Hypotheses of boreotropical origin, land bridge migration, and out-of-Tibet have been proposed to explain the formation of the discontinuous distribution pattern. The distribution of <em>Lonicera</em> shows a typical Europe-Asia-North America discontinuous distribution, which makes for a good case study to investigate the above three hypotheses. In this study, we inferred the phylogeny based on plastid genomes and a nuclear data set with broad taxon sampling, covering 83 species representing two subgenera and four sections. Both nuclear and plastid phylogenetic analyses found section <em>Isika</em> polyphyletic, while sections <em>Nintooa</em>, <em>Isoxylosteum</em>, and <em>Coelxylosteum</em> were monophyletic in subgenus <em>Chamaecerasus</em>. Based on the nuclear and chloroplast phylogeny, we suggest transferring L. <em>maximowiczii</em> and L. <em>tangutica</em> into section <em>Nintooa</em>. Reconstruction of ancestral areas suggests that <em>Lonicera</em> originated in the Qinghai-Tibetan Plateau (QTP) and/or Asia, and subsequently dispersed to other regions. The aridification of the Asian interior may have facilitated the rapid radiation of <em>Lonicera</em> in the region. At the same time, the uplifts of the Tibetan Plateau appear to have triggered the spread and recent rapid diversification of the genus on the QTP and adjacent areas. Overall, our results deepen the understanding of the evolutionary diversification history of <em>Lonicera</em>.</p>
Capturing single-copy nuclear genes, organellar genomes, and nuclear ribosomal DNA from deep genome skimming data for plant phylogenetics: A case study in Vitaceae
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Data from: Extensive allopolyploidy in the neotropical genus Lachemilla (Rosaceae) revealed by PCR ‐based target enrichment of the nuclear ribosomal DNA cistron and plastid phylogenomics
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New insights into infrageneric relationships of Lonicera (Caprifoliaceae) as revealed by nuclear ribosomal DNA cistron data and plastid phylogenomics
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Supplementary material 1 from: Tedersoo L, Liiv I, Kivistik PA, Anslan S, Kõljalg U, Bahram M (2016) Genomics and metagenomics technologies to recover ribosomal DNA and single-copy genes from old fruit-body and ectomycorrhiza specimens. MycoKeys 13: 1-20. https://doi.org/10.3897/mycokeys.13.8140
Full information and metadata about the genomic and metagenomic samples : Explanation note: Detailed information about metadata, DNA quality and genomic/metagenomic results of fruit-body and EcM root tip samples.
Fig. 3 in A phylogeny of Sericini with particular reference to Chinese species using mitochondrial and ribosomal DNA (Coleoptera: Scarabaeidae)
Fig. 3 Projections of phylogenetic relationships of selected Sericina clades into geographical space, illustrating the spatial within clade divergence between Himalayan and Chinese lowland species. a Sericina subclade 2 (Fig. 2), the North American clade is not shown, b detail of
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