Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
21
datasets available to search
ShareScore release 0.9.0
Dataset results
21 results for “sample preservation”
Mohonk Preserve Stream Water Quality Invasive Species and Macroinvertebrate Sampling in from 2017-Present
The mission of the Mohonk Preserve is to protect the Shawangunk Mountains region and inspire people to care for, enjoy, and explore their natural world. Among these 8,000 acres are the vernal pools, permanent springs, tributaries, Humpo Marsh, and the Humpo Kill, and parts of the Kleine Kill and Coxing Kill watersheds within the Hudson River Drainage Basin. Not only are the areas around the Shawangunks established habitats for New York State (NYS) protected species, including an Audubon-designated Important Bird Area, but the watershed also encapsulates more than one agricultural land use area, as well as Rondout Creek, which is an important waterway for the New York City water supply. A conservation plan must be implemented in these areas in particular, keeping in line with the Mohonk Preserves goal to conserve the Shawangunk region for both humans and the greater ecosystem within it. Recognizing the immediate and long-term conservation needs of the streams in this region by employing volunteer data collection will be a catalyst to the Preserves understanding of which environmental threats of this area should be prioritized. The StreamWatch citizen science program will be the newest addition to an array of volunteer research areas, which include collection of weather data, phenology observations, monitoring of peregrine falcon breeding activities, and monitoring of fall hawk migration. Using concise stream monitoring protocol designed for volunteer safety and maximum data accuracy, StreamWatch will evaluate water quality using an array of parameters. Following thorough observation and assessment (which will include analyzing appearance and smell of the water, shape of the stream, canopy cover, nearby land uses, recent weather, and presence of riparian vegetation including invasive species) water quality will be evaluated by means of temperature, dissolved oxygen, pH, and turbidity measurements, in addition to a macroinvertebrate count. Width and depth will also be
Text-fig. 5. Plant fragments from Govone with evidence of preserved cuticle. a: Decussate pair of leaves of "Thuja" saviana (C.T.GAUDIN) C.T.GAUDIN with a window (arrow) opened in the brownish cuticle, showing the yellowish mesophyll cells and some possible resin canals (dark), MGPT-PU141094. b: Angiosperm leaf fragment (from sample MGPT-PU141017) under the stereomicroscope, showing the blackish compressed mesophyll on the right and patches of cleaned, yellowish cuticle at the top (arrow). Scale bar 1 mm. in Remains Of A Subtropical Humid Forest In A Messinian Evaporitebearing Succession At Govone, Northwestern Italy - Preliminary Results
Text-fig. 5. Plant fragments from Govone with evidence of preserved cuticle. a: Decussate pair of leaves of "Thuja" saviana (C.T.GAUDIN) C.T.GAUDIN with a window (arrow) opened in the brownish cuticle, showing the yellowish mesophyll cells and some possible resin canals (dark), MGPT-PU141094. b: Angiosperm leaf fragment (from sample MGPT-PU141017) under the stereomicroscope, showing the blackish compressed mesophyll on the right and patches of cleaned, yellowish cuticle at the top (arrow). Scale bar 1 mm.
FIGURE 3. Trackway block samples after 3 in Towards sustainable treatments to preserve fossils from weathering, as part of the garden redevelopment project at the Natural History Museum
FIGURE 3. Trackway block samples after 3 treatments with CaLoSiL® E25 (no TiO2) at x10000 magnification, top left: ornithopod trackway before treatment; bottom left: ornithopod trackway after treatment; top right: theropod trackway before treatment; bottom right: theropod trackway after treatment. EHT 6.00kV, Signal A SE2, WD 8.1 mm, Magnification 10000 x.
Text-fig. 6. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal (a, b, d, e: sample 141, c: sample 300); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0563) through the median plane of the seed (holotype, S174178) showing the palisade-shaped cells of exotesta and collapsed inner parts of seed coat, raised exotestal tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and remains of cellular nutritive tissue. b) Longitudinal orthoslice (xz0659) through the micropylar region perpendicular to the median plane showing the exotestal tissue surrounding the transverse micropyle slit (mi). c) Longitudinal orthoslice (yz0500) through the median plane of the seed (S175095) showing raised tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and poorly preserved cells of the seed coat. d) Longitudinal orthoslice (xz0810) through middle part of seed perpendicular to the median plane (S174178) showing seed coat and remains of cellular nutritive tissue. e) Tangential and longitudinal orthoslice (xz0162) through the seed coat of holotype (S174178) showing thickened, undulate cell walls of exotesta (ex) and the short, thin-walled cells of tegmen (te) with a finely striate wrinkled surface. Scale bars = 250 µm (a, c, d); 125 µm (b, e). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 6. Reyispermum parvum gen. et sp. nov. seeds from the Early Cretaceous Vale de Água locality, Portugal (a, b, d, e: sample 141, c: sample 300); Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0563) through the median plane of the seed (holotype, S174178) showing the palisade-shaped cells of exotesta and collapsed inner parts of seed coat, raised exotestal tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and remains of cellular nutritive tissue. b) Longitudinal orthoslice (xz0659) through the micropylar region perpendicular to the median plane showing the exotestal tissue surrounding the transverse micropyle slit (mi). c) Longitudinal orthoslice (yz0500) through the median plane of the seed (S175095) showing raised tissue (arrow head) immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and poorly preserved cells of the seed coat. d) Longitudinal orthoslice (xz0810) through middle part of seed perpendicular to the median plane (S174178) showing seed coat and remains of cellular nutritive tissue. e) Tangential and longitudinal orthoslice (xz0162) through the seed coat of holotype (S174178) showing thickened, undulate cell walls of exotesta (ex) and the short, thin-walled cells of tegmen (te) with a finely striate wrinkled surface. Scale bars = 250 µm (a, c, d); 125 µm (b, e).
Text-fig. 9. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0769) through the median plane of the seed (S174345) showing expanded exotestal cells (ex, arrow head) in the hilar region and well-preserved cellular nutritive tissue with an embedded tiny embryo (asterisk markes the margin of the embryo); note well-developed mesotesta (me) on the raphal side of seed. b) Longitudinal orthoslice (yz0750) in the median plane showing detail of micropylar part of seed with well-preserved exotesta (ex) and mesotesta (me) and tiny embryo with two rudimentary cotyledons (asterisks mark the margin of the cotyledons); note numerous granular bodies in the cellular nutritive tissue that are probably the remains of protein and lipid bodies. c) Transverse orthoslice (xy0311) through seed below hilum showing the bulging exotesta (arrow heads) (S174472); note that the exotestal cells in this region have thinner walls (arrows). d) Transverse orthoslice (xy0900) through the middle of a seed showing the uneven thickening of the anticlinal walls of the exotestal cells (ex), which are thicker towards the outside and very thin towards the inside; note also mesotesta (me) and the well-preserved cellular nutritive tissue (S174472). Scale bars = 500 µm (a, d); 250 µm (b, c). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 9. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz0769) through the median plane of the seed (S174345) showing expanded exotestal cells (ex, arrow head) in the hilar region and well-preserved cellular nutritive tissue with an embedded tiny embryo (asterisk markes the margin of the embryo); note well-developed mesotesta (me) on the raphal side of seed. b) Longitudinal orthoslice (yz0750) in the median plane showing detail of micropylar part of seed with well-preserved exotesta (ex) and mesotesta (me) and tiny embryo with two rudimentary cotyledons (asterisks mark the margin of the cotyledons); note numerous granular bodies in the cellular nutritive tissue that are probably the remains of protein and lipid bodies. c) Transverse orthoslice (xy0311) through seed below hilum showing the bulging exotesta (arrow heads) (S174472); note that the exotestal cells in this region have thinner walls (arrows). d) Transverse orthoslice (xy0900) through the middle of a seed showing the uneven thickening of the anticlinal walls of the exotestal cells (ex), which are thicker towards the outside and very thin towards the inside; note also mesotesta (me) and the well-preserved cellular nutritive tissue (S174472). Scale bars = 500 µm (a, d); 250 µm (b, c).
Text-fig. 8. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025) (a, b, d, e), and the Vale de Água locality (sample 333) (c, f, g), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a) Smaller seed in lateral view showing the bulging region (arrow head) close to hilum (S174467). b) Cut volume rending (cut at yz1032) of seed in (8a) showing the expanded cells of exotesta immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and the well preserved nutritive tissue. c) Small seed in lateral view showing the bulging exotesta (arrow head) close to hilum (S175046). d, e) Larger seed in lateral (d) and raphal (e) view (S174035). f) Detail of seed in (8c) showing the micropylar slit (mi) above the hilum (hi) and bulging exotestal tissue (arrow head) in which the walls of the exotestal cells have straight anticlinal walls. g) Surface of seed in (8c) showing the raised undulate anticlinal walls of the exotestal cells. Scale bars = 500 µm (a–e); 250 µm (f); 125 µm (g). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 8. Lusitanispermum choffatii gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025) (a, b, d, e), and the Vale de Água locality (sample 333) (c, f, g), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, volume renderings). a) Smaller seed in lateral view showing the bulging region (arrow head) close to hilum (S174467). b) Cut volume rending (cut at yz1032) of seed in (8a) showing the expanded cells of exotesta immediately adjacent to the lower edge of the hilum (hi), micropyle (mi) and the well preserved nutritive tissue. c) Small seed in lateral view showing the bulging exotesta (arrow head) close to hilum (S175046). d, e) Larger seed in lateral (d) and raphal (e) view (S174035). f) Detail of seed in (8c) showing the micropylar slit (mi) above the hilum (hi) and bulging exotestal tissue (arrow head) in which the walls of the exotestal cells have straight anticlinal walls. g) Surface of seed in (8c) showing the raised undulate anticlinal walls of the exotestal cells. Scale bars = 500 µm (a–e); 250 µm (f); 125 µm (g).
Sampling a pika’s pantry: Temporal shifts in nutritional quality & over-winter preservation of American pika food caches
Climate change is increasing temperature, decreasing precipitation, and increasing atmospheric CO2 concentrations in many ecosystems. As atmospheric carbon rises, plants may increase carbon-based defenses such as phenolics, thereby potentially affecting food quality, foraging habits, and habitat suitability for mammalian herbivores. In alpine habitats, the American pika (Ochotona princeps) is a model species for studying effects of changing plant chemistry on mammals. To survive between growing seasons, pikas cache “haypiles” of plants rich in phenolics. Although they are acutely toxic to pikas, phenolic compounds help plants retain biomass and nutrition during storage, and they break down over time. Alpine avens (Geum rossii, Rosales: Rosaceae) is a high-phenolic plant species that comprises up to 75% of pika winter diet in Colorado. Here, we tested the hypothesis that contemporary climate change has affected the nutritional value of Alpine avens to pikas in the last 30 years. Specifically, we compared phenolic activity, nutritional quality, and overwinter preservation of plants collected at Niwot Ridge, Colorado (USA) in 1992 to those collected between 2010 – 2018, spanning nearly three decades of climate change. Phenolic activity increased in alpine avens since 1992, while fiber and nitrogen content decreased. Importantly, overwinter preservation of plant biomass also increased, particularly on windblown slopes without long-lasting snow cover. Previous studies indicate that pikas at this site still depend on alpine avens in their winter food caches. Increasing phenolic content in alpine avens could therefore enhance the preservation of haypiles over winter; however, if pikas must further delay consuming these plants to avoid acute toxicity, then he nutritional gains from enhanced preservation may not be beneficial. This study provides important insights into how changing plant chemistry will affect mammalian herbivores in the future.
Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river
<p>Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain to be addressed. We investigated the effect of sampling me‐ dium and time, and preservation methods, on fish detection performance based on eDNA metabarcoding of neotropical freshwater samples. Water and sediment sam‐ ples were collected from 11 sites along the Jequitinhonha River, Southeastern Brazil; sediment samples were stored in ethanol, while the same amounts of water per sam‐ ple (3 L) were stored in a cool box with ice, as well as by adding the cationic surfactant benzalkonium chloride (BAC). Sediment and water samples yielded a similar amount of fish MOTUs (237 vs. 239 in the first sampling event, and 153 vs. 142 in the second sampling event). Water stored in ice provided better results than those preserved in BAC (239 and 142 vs. 194 and 71 MOTUs). While documenting the effectiveness of eDNA surveys as practical tools for fish biodiversity monitoring in poorly accessible areas, we showed that keeping water samples cooled results in greater eDNA recovery and taxon detection than by adding cationic surfactants (BAC) as sample pre‐ servatives. Furthermore, by comparing two sets of samples collected from the same locations at a 3‐week interval, we highlight the importance of conducting multiple sampling events when attempting to recover a realistic picture of fish assemblages in lotic systems.</p>
Sample preservation methods for nitrous oxide concentration and isotope ratio measurements in aquatic environments
<p>This is the raw data for the method paper submitted in Limnology and Oceanography: Methods titled "Sample preservation methods for nitrous oxide concentration and isotope ratio measurements in aquatic environments".</p>
Structure preserving adversarial generation of labeled training samples for single cell segmentation
<p>Supplementary package (dataset, generated data and code) for the article: "<strong>Structure preserving adversarial generation of labeled training samples for single cell segmentation</strong>". (Cell Reports Methods, 2023 - under submission)</p> <p>Content of the package:</p> <ul> <li>datasets.zip: the images and annotations for the fallopian tube and salivary gland dataset.</li> <li>mask_quality_experiment.zip: the generated masks using SIMCEP and the generated microscopy images using pix2pix using the SIMCEP masks.</li> <li>pix2pix_models.zip: the models for fold 0 for both datasets. It can be used to synthesize new images.</li> <li>StyleGAN2-ada_models.zip: the models for synthesizing new masks.</li> <li>code.zip: a github repository (named .git) for executing the pipeline.</li> </ul>
Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river
Open the record for dataset details and reuse information.
Data from: Time is of the essence: using archived samples to develop a GT-seq panel to preserve continuity of ongoing genetic monitoring
Open the record for dataset details and reuse information.
Unwanted loss of volatile organic compounds (VOCs) during in situ chemical oxidation sample preservation: Mechanisms and solutions
Open the record for dataset details and reuse information.
Optimising recovery of DNA from minimally-invasive sampling methods: efficacy of buccal swabs, preservation strategy and DNA extraction approaches for amphibian studies_Dataset_Rscript
<p>Datasets and Rscript associated with paper draft titled: "<span>Optimising recovery of DNA from minimally-invasive sampling methods: efficacy of buccal swabs, preservation strategy and DNA extraction approaches for amphibian studies".</span></p> <p> </p> <p>Abstract: <span>Studies in evolution, ecology and conservation are increasingly based on genetic and genomic inferences. With increased focus on molecular approaches, ethical concerns about destructive or more invasive techniques need to be considered, with a push for minimally invasive sampling to be optimised. Buccal swabs have been increasingly used to collect DNA in a number of taxa, including amphibians.<span> </span>However, DNA yield and purity from swabs is often low, limiting its use. In this study we compare different types of swabs, preservation method and storage, and DNA extraction technique in three case studies to assess the optimal approach for recovering DNA in anurans. Out of the five different types of swab that we tested, Isohelix MS-02 and Rapidry swabs generated higher DNA yields than other swabs. When comparing storage buffers, ethanol is a better preservative than a non-alcoholic alternative. Dried samples resulted in similar or better final DNA yields than ethanol-fixed samples if kept cool. DNA extraction via a Qiagen</span><span>™</span><span> DNeasy Blood and Tissue Kit and McHale’s salting out extraction method resulted in similar DNA yields but the Qiagen</span><span>™</span><span> kit extracts contained less contamination. We also found that samples produce better DNA recovery if frozen as soon as possible after collection. We provide recommendations for sample collection and extraction under different conditions, including budgetary considerations, size of individual sampled, access to cold storage facilities, and DNA extraction methodology. Maximising efficacy of all of these factors for better DNA recovery will allow buccal swabs to be used for genetic and genomic studies in a range of vertebrates.</span></p>
Text-fig. 12. Silutanispermum kvacekiorum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz1170) through the median plane of the seed (S170238) in the micropylar region showing exotestal cells lining the micropylar slit (mi) and hilum (hi); note the well-preserved mesotestal cells (me). b) Longitudinal orthoslice (xz0805) of seed perpendicular to the median plane through the micropylar region showing the transverse micropylar slit (mi) lined by radiating exotestal cells; note abundant mesotestal cells (me). c) Transverse orthoslice (xy0768) through seed below hilum and micropyle showing exotesta (ex) and mesotesta (me) that is strongly developed along the raphe (ra) (S174352). d) Transverse orthoslice (xy2113) through middle of the seed showing well-preserved cellular nutritive tissue with empty cells; note that the raphe (ra) is enclosed in mesotestal tissue (S174352). e) Longitudinal orthoslice (yz0970) through seed coat showing exotesta (ex) of tall palisade-shaped cells and thick mesotesta (me) of low cells (S174352). Scale bars = 500 µm (a, c, d); 250 µm (b); 125 µm (e). in Extinct Taxa Of Exotestal Seeds Close To Austrobaileyales And Nymphaeales From The Early Cretaceous Of Portugal
Text-fig. 12. Silutanispermum kvacekiorum gen. et sp. nov. seeds from the Early Cretaceous Famalicão locality (sample 025), Portugal; Synchrotron radiation X-ray tomographic microscopy (SRXTM, orthoslices). a) Longitudinal orthoslice (yz1170) through the median plane of the seed (S170238) in the micropylar region showing exotestal cells lining the micropylar slit (mi) and hilum (hi); note the well-preserved mesotestal cells (me). b) Longitudinal orthoslice (xz0805) of seed perpendicular to the median plane through the micropylar region showing the transverse micropylar slit (mi) lined by radiating exotestal cells; note abundant mesotestal cells (me). c) Transverse orthoslice (xy0768) through seed below hilum and micropyle showing exotesta (ex) and mesotesta (me) that is strongly developed along the raphe (ra) (S174352). d) Transverse orthoslice (xy2113) through middle of the seed showing well-preserved cellular nutritive tissue with empty cells; note that the raphe (ra) is enclosed in mesotestal tissue (S174352). e) Longitudinal orthoslice (yz0970) through seed coat showing exotesta (ex) of tall palisade-shaped cells and thick mesotesta (me) of low cells (S174352). Scale bars = 500 µm (a, c, d); 250 µm (b); 125 µm (e).
Supplementary material 2 from: Baricevic A, Chardon C, Kahlert M, Karjalainen SM, Pfannkuchen DM, Pfannkuchen M, Rimet F, Tankovic MS, Trobajo R, Vasselon V, Zimmermann J, Bouchez A (2022) Recommendations for the preservation of environmental samples in diatom metabarcoding studies. Metabarcoding and Metagenomics 6: e85844. https://doi.org/10.3897/mbmg.6.85844
Data 2
Supplementary material 3 from: Baricevic A, Chardon C, Kahlert M, Karjalainen SM, Pfannkuchen DM, Pfannkuchen M, Rimet F, Tankovic MS, Trobajo R, Vasselon V, Zimmermann J, Bouchez A (2022) Recommendations for the preservation of environmental samples in diatom metabarcoding studies. Metabarcoding and Metagenomics 6: e85844. https://doi.org/10.3897/mbmg.6.85844
Data 3
Supplementary material 1 from: Baricevic A, Chardon C, Kahlert M, Karjalainen SM, Pfannkuchen DM, Pfannkuchen M, Rimet F, Tankovic MS, Trobajo R, Vasselon V, Zimmermann J, Bouchez A (2022) Recommendations for the preservation of environmental samples in diatom metabarcoding studies. Metabarcoding and Metagenomics 6: e85844. https://doi.org/10.3897/mbmg.6.85844
Data 1
Data from: Preservation of RNA and DNA from mammal samples under field conditions
Open the record for dataset details and reuse information.
Comparison of microRNA expression using different preservation methods of matched psoriatic skin samples
GEO Series GSE28729. Homo sapiens; Murid betaherpesvirus 1; Mus musculus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Rattus norvegicus; JC polyomavirus; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Betapolyomavirus macacae. 75 samples. Type: Non-coding RNA profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.