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ShareScore release 0.9.0
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14 results for “secretory pathway”
Fluorescence lifetime imaging of pH along the secretory pathway
<p>Many cellular processes are dependent on correct pH levels, and this is especially important for the secretory pathway. Defects in pH homeostasis in distinct organelles cause a wide range of diseases, including disorders of glycosylation and lysosomal storage diseases. Ratiometric imaging of the pH-sensitive mutant of green fluorescent protein (GFP), pHLuorin, has allowed for targeted pH measurements in various organelles, but the required sequential image acquisition is intrinsically slow and therefore the temporal resolution unsuitable to follow the rapid transit of cargo between organelles. We therefore applied fluorescence lifetime imaging microscopy (FLIM) to measure intraorganellar pH with just a single excitation wavelength. We first validated this method by confirming the pH in multiple compartments along the secretory pathway. Then, we analyze the dynamic pH changes within cells treated with Brefeldin A, a COPI coat inhibitor. Finally, we followed the pH changes of newly-synthesized molecules of the inflammatory cytokine tumor necrosis factor (TNF)-α while it was in transit from the endoplasmic reticulum via the Golgi to the plasma membrane. The toolbox we present here can be applied to measure intracellular pH with high spatial and temporal resolution, and can be used to assess organellar pH in disease models.</p>
Results for Genome scale modeling of the protein secretory pathway reveals novel targets for improved recombinant protein production in yeast
<p>This dataset contains the original results for the paper "Genome scale modeling of the protein secretory pathway reveals novel targets for improved recombinant protein production in yeast". To reproduce all figures in this paper, this dataset is required. The scripts can be found in Github (https://github.com/SysBioChalmers/pcSecYeast)</p>
The ribosome collision sensor Hel2 functions as preventive quality control in the secretory pathway
GEO Series GSE156535. Saccharomyces cerevisiae. 24 samples. Type: Other.
BET bromodomain inhibitors synergize with ATR inhibitors to induce DNA damage, apoptosis, senescence-associated secretory pathway and ER stress in Myc- induced lymphoma cells
GEO Series GSE74873. Mus musculus. 8 samples. Type: Expression profiling by array.
Inferring secretory and metabolic pathway activity from omic data with secCellFie
GEO Series GSE248376. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Active Creb3L1 can upregulate secretory pathway genes in HeLa cells
GEO Series GSE23334. Homo sapiens. 6 samples. Type: Expression profiling by array.
Unique human beta-cell senescence-associated secretory phenotype (SASP) reveal conserved signaling pathways and heterogeneous factors
GEO Series GSE162521. Homo sapiens. 27 samples. Type: Protein profiling by protein array.
Activation of Notch1 and Notch3 Skews Human Airway Basal Cell Differentiation Toward a Secretory Pathway
GEO Series GSE53537. Homo sapiens. 5 samples. Type: Expression profiling by array.
CrebA is a major and direct regulator of secretory pathway gene expression
GEO Series GSE23346. Drosophila melanogaster. 6 samples. Type: Expression profiling by array.
CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway
<p><span><span><span><span><span><span><span><span><span><span><span>Neurexins are presynaptic adhesion molecules that shape the molecular composition of synapses. Diversification of neurexins in numerous isoforms is believed to confer synapse-specific properties by engaging with distinct ligands. For example, a subset of neurexin molecules carry a heparan sulfate (HS) glycosaminoglycan that controls ligand binding, but how this posttranslational modification is controlled is not known. Here, we observe that CA10, a ligand to neurexin in the secretory pathway, regulates neurexin-HS formation. CA10 is exclusively found on non-HS neurexin and CA10 expressed in neurons is sufficient to suppress HS addition and attenuate ligand binding and synapse formation induced by ligands known to recruit HS. This effect is mediated by a direct interaction in the secretory pathway that blocks the primary step of HS biosynthesis: xylosylation of the serine residue. NMR reveals that CA10 engages residues on either side of the serine that can be HS-modified, suggesting that CA10 sterically blocks xylosyltransferase access in Golgi. These results suggest a mechanism for the regulation of HS on neurexins and exemplify a new mechanism to regulate site-specific glycosylations.</span></span></span></span></span></span></span></span></span></span></span></p>
CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway
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Secretory pathway differences between HEK293 and CHO enable rescue of difficult to express human proteins
GEO Series GSE157729. Homo sapiens; Cricetulus griseus. 78 samples. Type: Expression profiling by high throughput sequencing.
Aryl hydrocarbon receptor dynamically regulates inflammatory and secretory gene pathways in decidual endometrial stromal cells
GEO Series GSE118413. Homo sapiens. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Aryl hydrocarbon receptor dynamically regulates immunological and secretory gene pathways in decidual endometrial stromal cells
GEO Series GSE114552. Homo sapiens. 10 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.