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15 results for “seminal fluid proteins”
Data and code for: Recombinant venom proteins in insect seminal fluid reduces female lifespan
<p>Publication: https://doi.org/10.1101/2024.01.14.575309</p> <p>This repository contains all the data as well as the scripts used for the analysis and visualisation. </p> <table> <tbody> <tr> <td><strong>File</strong></td> <td><strong>Description</strong></td> </tr> <tr> <td>TMT.ipynb</td> <td>The Jupyter notebook containing the Python code for statistical analysis and figure generation. Scripts are sectioned in the order that results are presented in the study, with sub-headings and figure numbers where appropriate.</td> </tr> <tr> <td>actingal4.csv</td> <td>Offspring phenotype data from the UAS:venom x Act5C-GAL4 assay (Results: Testing functional expression of recombinant venoms).</td> </tr> <tr> <td>femalelifespan_1-1.csv</td> <td>Female lifespan data from the 1:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored. </td> </tr> <tr> <td>femalelifespan_3-1.csv</td> <td>Female lifespan data from the 3:1 male:female mating assay (Results: TMT males reduce the lifespan of mated females). Time = days since initial male exposure until death. Status: 1 = death observed; 0 = censored. </td> </tr> <tr> <td>singlemating.csv</td> <td>Data from the single-pair courtship assay. 1 = successful courtship observed; 0 = successful courtship not observed.</td> </tr> <tr> <td>competitivemating.csv</td> <td>Offspring genotype data from the competitive mating assay. Female = group of offspring from a given mother. white = positive result from gDNA PCR using the 'white' primers (supplementary table S2). TMT = positive result from gDNA PCR using the 'UAS' primers (supplementary table S2).</td> </tr> <tr> <td>malelifespan.csv</td> <td>TMT male lifespan data from the male longevity assay. Time = days since eclosion. Status: 1 = death observed; 0 = censored. </td> </tr> </tbody> </table> <p> </p> <p>TMT-GAMA.db and TMT-lethality.db contains data from the GAMA model (doi:10.5281/zenodo.11439089), which is accessed by the Jupyter notebook.</p> <table> <tbody> <tr> <td><strong>Table</strong></td> <td><strong>Description</strong></td> </tr> <tr> <td>TMT_step_output</td> <td>Each simulation will write key values to this table each cycle once transgenic males have begun to be released.</td> </tr> <tr> <td>TMT_female_output</td> <td>Each female will write key values to this table upon their death once transgeni males have begun to be released.</td> </tr> <tr> <td>TMT_PR50</td> <td>Each simulation will write to this table when/if the female population reaches 50% of its initial size.</td> </tr> <tr> <td>TMT_PR95</td> <td>Each simulation will write to this table when/if the female population reaches 95% of its initial size.</td> </tr> </tbody> </table>
Data from: A novel sperm-derived seminal fluid protein in Caenorhabditis nematodes
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Phenotypic data from seminal fluid protein Acp29AB knockdown experiments
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Sperm length and seminal fluid proteins promote male reproductive success in D. melanogaster
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Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation
<p>Despite holding a central role for fertilisation success, reproductive traits often show elevated rates of evolution and diversification. The rapid evolution of seminal fluid proteins (Sfps) within populations is predicted to cause mis-signalling between the male ejaculate and female reproductive tract between populations resulting in postmating prezygotic (PMPZ) isolation. Crosses between populations of <i>Drosophila montana</i> show PMPZ isolation in the form of reduced fertilisation success in both noncompetitive and competitive contexts. Here we test whether male ejaculate proteins deriving from either the accessory glands or the ejaculatory bulb differ between populations using liquid chromatography tandem mass spectrometry. We find more than 150 differentially abundant proteins between populations which may contribute to PMPZ isolation. These proteins include a number of proteases and peptidases, and several orthologs of <i>D. melanogaster</i> Sfps, all known to mediate fertilisation success and which mimic PMPZ isolation phenotypes. Males of one population typically produced greater quantities of Sfps and the strongest PMPZ isolation occurs in this direction. The accessory glands and ejaculatory bulb have different functions and the ejaculatory bulb contributes more to population differences than the accessory glands. Proteins with a secretory signal, but not Sfps, evolve faster than non-secretory proteins although the conservative criteria used to define Sfps may have impaired the ability to identify rapidly evolving proteins. We take advantage of quantitative proteomics data from three <i>Drosophila</i> species to determine shared and unique functional enrichments of Sfps that could be subject to selection between taxa and subsequently mediate PMPZ isolation. Our study provides the first high throughput quantitative proteomic evidence showing divergence of reproductive proteins implicated in the emergence of PMPZ isolation between populations.</p>
Plasmid sequences for: Recombinant venom proteins in insect seminal fluid reduces female lifespan
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Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation
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Non-adaptive molecular evolution of seminal fluid proteins in drosophila
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Data from: Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail
Seminal fluid proteins (SFPs) are components of the ejaculate that often induce drastic changes in female physiology, such as reducing remating rate or shortening longevity. There is compelling evidence that these functions make SFPs a determinant of male reproductive success, and some evidence that males can strategically invest in their differential production. However, SFP-mediated effects have received relatively little attention in simultaneous hermaphrodites, i.e. organisms that are male and female at the same time. Since this reproductive mode is widespread in animals and their SFPs have unique functions compared to separate-sexed species, examining SFPs of hermaphrodites would help generalize our understanding of the impact of SFPs. We therefore examined if individuals strategically alter seminal fluid production in response to mate availability and sperm competition in the freshwater snail Lymnaea stagnalis. We exposed snails to different social group sizes, and measured the expression of six SFP genes. We found that the snails plastically elevated SFP expression in the presence of at least one mating partner. Specifically, paired snails showed higher SFP expression than isolated snails, while SFP expression of snails exposed to sperm competition, i.e. in a larger group size, was equivalent to that of paired snails. Furthermore, five out of six SFP genes we examined responded to mate availability in a very similar way, implying that overall seminal fluid production increases when the snails have mating opportunities. The plastic expression of seminal fluid depending on mate availability supports that SFPs play important roles in post-copulatory processes in this hermaphroditic species.
Data from: Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)
Background: Seminal fluid proteins control many aspects of fertilization and in turn, they play a key role in post-mating sexual selection and possibly reproductive isolation. Because effective proteome profiling relies on the availability of high-quality DNA reference databases, our knowledge of these proteins is still largely limited to model organisms with ample genetic resources. New advances in sequencing technology allow for the rapid characterization of transcriptomes at low cost. By combining high throughput RNA-seq and shotgun proteomic profiling, we have characterized the seminal fluid proteins secreted by the primary male accessory gland of the migratory grasshopper (Melanoplus sanguinipes), one of the main agricultural pests in central North America. Results: Using RNA sequencing, we characterized the transcripts of ~ 8,100 genes expressed in the long hyaline tubules (LHT) of the accessory glands. Proteomic profiling identified 353 proteins expressed in the long hyaline tubules (LHT). Of special interest are seminal fluid proteins (SFPs), such as EJAC-SP, ACE and prostaglandin synthetases, which are known to regulate female oviposition in insects. Conclusions: Our study provides new insights into the proteomic components of male ejaculate in Orthopterans, and highlights several important patterns. First, the presence of proteins that lack predicted classical secretory tags in accessory gland proteomes is common in male accessory glands. Second, the products of a few highly expressed genes dominate the accessory gland secretions. Third, accessory gland transcriptomes are enriched for novel transcripts. Fourth, there is conservation of SFPs' functional classes across distantly related taxonomic groups with very different life histories, mating systems and sperm transferring mechanisms. The identified SFPs may serve as targets of future efforts to develop species- specific genetic control strategies.
Data from: A targeted in situ hybridization screen identifies putative seminal fluid proteins in a simultaneously hermaphroditic flatworm
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Data from: Plastic expression of seminal fluid protein genes in a simultaneously hermaphroditic snail
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Data from: Control of seminal fluid protein expression via regulatory hubs in D. melanogaster
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Data from: Genetic and environmental variation in transcriptional expression of seminal fluid proteins
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Data from: Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)
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