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Fig. 4 in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 4. Vizcainocypria viator gen. nov. sp. nov., male (MUVHNZY0011). A: A2. B: Right prehensile palp. C: Left prehensile palp. D: Hemipenis. E: Zenker organ. Scale bars: A–E = 50 µm.

Fig. 2 in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 2. Vizcainocypria viator gen. nov. sp. nov., female (MUVHNZY0012). A: A1 (arrow pointing to apical claw on penultimate segment). B: A2. C: Md coxa. D: Md palp. E: Detail of α and β setae. F: Mxl. Scale bars: A–F = 50 µm.

Fig. 3 in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 3. Vizcainocypria viator gen. nov. sp. nov., female (MUVHNZY0012). A: T1. B: T2. C: T3. D: CR. E: Caudal attachment. Scale bars: A–E = 50 µm.

Fig. 6 in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 6. Male copulatory organs (hemipenes) of different species of Cyclocyprididae. A: Cyclocypris, B: Cypria, C: Physocypria, D: Dentocypria, E: Keysercypria, F: Brasilocypria, G: Claudecypria, H: Vizcainocypria. Redrawn from Almeida et al. (2023): F, G; Karanovic (2011): C (P. bullata), E; Hartmann (1959): H (V. granadae); Meisch (2000): A, B (C. exsculpta, C. ophtalmica), C (P. kraepelini); Savatenalinton (2017): D; Smith and Janz (2008): B (C. matzkeae), C (P. nipponica, P. biwaensis); Wouters (1984): B (C. subsalsa). Scale bars are shown when available: D. smithi = 46 µm; C. ovum, C. ophtalmica, C. subsalsa, P. nipponica, P. biwaensis, D. mesquitai, B. pea, B. alisonae, C. mesquitai, C. rochei, V. viator = 50 µm; C. matzkeae, P. bullata, K. affinis, K. deformis = 100 µm.

Fig. 1 in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 1. Vizcainocypria viator gen. nov. sp. nov., female (A, C–G, I), male (B, H). A: Mature female specimen. B: Mature male specimen. C: CpL from right side (MUVHNZY0020). D: CpF (MUVHNZY0019). E: CpD (MUVHNZY0018). F: LVi (MUVHNZY0016). G: Detail of the internal tooth (MUVHNZY0016). H: RVi (MUVHNZY0011). I: Detail of the tubercles on RV margin (MUVHNZY0017). Scale bars: A–F, H = 200 µm; G = 10 µm; I = 5 µm.

Fig. 5. Maximum likelihood tree for 28S in Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 5. Maximum likelihood tree for 28S (A) and COX1 (B) genes. Red branches indicate the presence of tubercles on the RV margin.

Fig. 8. The IL-6 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 8. The IL-6 expression levels of Japanese eel and giant mottled eel reared in different spectra. W: white light; B: blue light; G: green light; R: red light; black: dark. Different letters indicate significant differences between different spectra groups of the same eel species (p <0.05).

Fig. 7 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 7. The LZM expression levels of Japanese eel and giant mottled eel reared in different spectra. W: white light; B: blue light; G: green light; R: red light; black: dark. Different letters indicate significant differences between different spectra groups of the same eel species (p <0.05).

Fig. 6 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 6. The SOD expression levels of Japanese eel and giant mottled eel reared in different light spectra. W: white light; B: blue light; G: green light; R: red light; black: dark. Different letters indicate significant differences between different spectra groups of the same eel species (p <0.05).

Fig. 5 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 5. The body weight of giant mottled eel reared in different light spectra for 12 weeks. W: white light; Black: dark; B: blue light; G: green light; R: red light. Different letters indicate significant differences between groups of the same week (p <0.05).

Fig. 3 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 3. The body weight of Japanese eel reared in different light spectra for 12 weeks. W: white light; Black: dark; B: blue light; G: green light; R: red light.

Fig. 2 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 2. The total length of Japanese eel reared in different light spectra for 12 weeks. W: white light; Black: dark; B: blue light; G: green light; R: red light.

Fig. 4 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 4. The total length of giant mottled eel reared in different light spectra for 12 weeks. W: white light; Black: dark; B: blue light; G: green light; R: red light. Different letters indicate significant differences between groups of the same week (p <0.05).

Fig. 1 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 1. Graph of a set of recirculating aquaculture systems (RAS) used in this study. The five tanks were each 40 L in volume and covered by a black board. W: white light; R: red light (622 nm); G: green light (517 nm); B: blue light (467 nm).

Fig. 9 in Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 9. The POD expression levels of Japanese eel and giant mottled eel reared in different spectra. W: white light; B: blue light; G: green light; R: red light; black: dark. Different letters indicate significant differences between different spectra groups of the same eel species (p <0.05).

Fig. 4 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 4. Comparisons of ΔCT values of LOC111853410, kera, and dcn between the "S-Female" (n = 7, four repeats) and "S-Male" (n = 5, four repeats) groups for assessing the threshold for sex typing. (A) LOC111853410, (B) kera, (C) dcn. A solid black circle represented one ΔCT value. The "S-Female" meant the female silver eels, and the "S-Male" meant the male silver eels. For each gene, there were 28 and 20 solid black circles in the "S-Female" group and the "S-Male" group, respectively. The solid red line meant the inferred threshold of ΔCT for sex typing.

Fig. 3 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 3. Comparisons of relative expression levels of four genes possibly higher expressed in the pectoral fins of male silver eels between the "S-Female" (n = 7, four repeats) and "S-Male" (n = 5, four repeats) groups. (A) kera, (B) dcn, (C) cited1, (D) LOC104575574. The arp was a housekeeping gene, and two female samples (F1 and F2) were used as the internal control for calculating relative expression levels. The y-axis was in log2 scale. The "S-Female" meant the female silver eels, and the "S-Male" meant the male silver eels. The solid black line represented the median value, and the solid black circle represented the outliers. Asterisks indicated significant differences (**P <0.01, ***P <0.001) in relative expression levels between two groups.

Fig. 2 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 2. Comparisons of relative expression levels of six genes possibly higher expressed in the pectoral fins of female silver eels between the "S-Female" (n = 7, four repeats) and "S-Male" (n = 5, four repeats) groups. (A) LOC108249696, (B) LOC110515957, (C) egr3, (D) cipc, (E) LOC111853410, (F) cttn. The arp was a housekeeping gene, and two female samples (F1 and F2) were used as the internal control for calculating relative expression levels. The y-axis was in log2 scale. The "S-Female" meant the female silver eels, and the "S-Male" meant the male silver eels. The solid black line represented the median value, and the solid black circle represented the outliers. Asterisks indicated significant differences (**P <0.01, ***P <0.001) in relative expression levels between two groups.

Fig. 1 in Identification of Sexually Dimorphic Genes in Pectoral Fin as Molecular Markers for Assessing the Sex of Japanese Silver Eels ().

Fig. 1. Scheme of the MOLAS database for the Japanese eel. Annotated information of the assembled transcripts, including DNA sequence, amino acids sequence, open reading frames, signal peptides, transmembrane domains, gene ontology, hit KEGG pathway, and FPKM values, can be searched by the names of genes or the transcript ID in the Full-text search. A sequence of DNA can also be used to find the transcript with high similarity through the Sequence Search/BLAST. Moreover, the Pairwise Comparison can compare the differences in expression levels of the transcripts between two different libraries or two library groups, and then the differentially expressed genes can be summarized to a gene list. Furthermore, the Import Genelist can analyze the protein function, gene ontology enrichment, heatmap of expression, and hit terms on a KEGG pathway for a gene list. Additionally, the Clustering can be used to categorize the expressional patterns of transcripts between two different libraries or two library groups. Finally, the KEGG GlobalView and Gene List Analysis can see the hit terms of transcripts on the map of KEGG pathway and compare the different gene lists by Venn diagrams, respectively.