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17 results for “shikonin”
Characterization of triacylglycerol secretion with shikonin derivatives in Lithospermum erythrorhizon
<p><span>This dataset contains data from electron microscopy and biochemical analyses described in the paper: "Tatsumi, K., et al. Excretion of triacylglycerol as a matrix lipid facilitating apoplastic accumulation of a lipophilic metabolite shikonin" submitted to Journal of Experimental Botany. The raw data underlying the paper are given as separate excel files, which are deposited to 'Data'. The Excel file 'result03_positive_181119_Yazaki_Lab_5.xlsx' is the data of LC-MS for Figure 3 of the above paper. Another Excel file 'GC-FID_rawdata-processed.xlsx' is the raw data for the quantitative analysis of fatty acids by GC-FID as well as the processed data shown in Figure 5 and Supporting Figure S7. The third Excel file 'lipidome_data_processed.xlsx' is the data of lipidome analysis shown in Supporting Figures S3, S5, and S6.</span></p>
Characterization of triacylglycerol secretion with shikonin derivatives in Lithospermum erythrorhizon
Open the record for dataset details and reuse information.
Fig. 5 in Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis
Fig. 5. Multiple amino acid sequence alignment of geranylhydroquinone 3″-oxygenases. Alignments of cytochrome P450s CYP76B100 and CYP76B101 from L. erythrorhizon and CYP76B74 from A. euchroma are illustrated. Red boxes indicate identical amino acid residues; Red fonts with no box indicate identical residues for at least two enzyme sequences. The highly conserved I-helix motif AGT(V)DTT, the K-helix motif KEA(T)L(V)R and the conserved haem binding domain PFGAGRRS (I)CPG are highlighted in blue. CYP76B74 (GenBank accession number: AZU97066) was functionally characterized as geranylhydroquinone 3″-hydroxylase from A. euchroma.
Fig. 2 in Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis
Fig. 2. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B100 in feeding experiments. (A) The HPLC chromatogram of authentic standard GHQ (black line), the extracts from a control yeast strain expressing AtCPR1 alone (green line), and the extracts from the engineered yeast strain co-expressing CYP76B100 and AtCPR1 (red line). The labelled peaks correspond to GHQ-3″-OH (1), and authentic substrate GHQ. (B) Oxidation at the C-3″ position of substrate GHQ to GHQ-3″-OH (1) catalyzed by CYP76B100. (C) The mass spectrum for the product 1 eluting at 21.1 min.
Fig. 1 in Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis
Fig. 1. The proposed biosynthetic pathway of shikonin in L. erythrorhizon. Single arrows represent one step reaction, while double arrows represent multiple step reactions. Dashed arrows signify undefined steps or the enzymes have not been verified yet. The steps with red-colored enzymes indicate the oxidation reactions of converting GHQ to GHQ-3″-OH or GHQ-3″-COOH by CYP76B100 and CYP76B101. PGT, geranyl diphosphate: 4-hydroxybenzoate 3-geranyltransferase; GBA, 3- geranyl-4-hydroxybenzoic acid; GHQ, geranylhydroquinone; GHQ-3″-OH, 3″-hydroxy-geranylhydroquinone; GHQ-3″-CHO, 3″-aldehyde-geranylhydroquinone; GHQ- 3″-COOH, 3″-carboxyl-geranylhydroquinone.
Fig. 4 in Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis
Fig. 4. HPLC analysis of CYP76B100 and CYP76B101 microsomal enzyme assays in vitro incubated with GHQ. Microsomal preparation containing AtCPR1 alone was used as a negative control (black line). Microsomal proteins were respectively prepared from the engineered yeast strain co-expressing CYP76B100 and AtCPR1 (green line), and the engineered yeast strain co-expressing CYP76B101 and AtCPR1 (blue line).
Inhibitory effect of shikonin on lung cancer cells
GEO Series GSE222640. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
RNA-Seq analysis of breast cancer cells after shikonin treatment
GEO Series GSE100687. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome and microRNA array analyses reveal a stimulatory effect of the phytochemical shikonin on epithelial–mesenchymal transition (EMT) in mouse skin [microRNA expression profile]
GEO Series GSE32695. Mus musculus. 4 samples. Type: Non-coding RNA profiling by array.
Transcriptome and microRNA array analyses reveal a stimulatory effect of the phytochemical shikonin on epithelial–mesenchymal transition (EMT) in mouse skin [gene expression profile]
GEO Series GSE32694. Mus musculus. 8 samples. Type: Expression profiling by array.
Shikonin inhibit progression of colon cancer
GEO Series GSE234709. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
Fig. 3 in Potential role of two cytochrome P450s obtained from Lithospermum erythrorhizon in catalyzing the oxidation of geranylhydroquinone during Shikonin biosynthesis
Fig. 3. LC-MS analysis of the biosynthetic products from GHQ catalyzed by CYP76B101 in feeding experiments. (A) The HPLC chromatogram of authentic standard GHQ (black line), the extracts from a control yeast strain expressing AtCPR1 alone (green line), and the extracts from the engineered yeast strain co-expressing CYP76B101 and AtCPR1 (blue line). The labelled peaks correspond to GHQ-3″-OH (2), GHQ-3″-COOH (3), and authentic substrate GHQ. (B) Oxidation at the C-3″ position of substrate GHQ to GHQ-3″-OH (2), to possible intermediate GHQ-3″-CHO (undetectable) and finally to GHQ-3″-COOH (3), all catalyzed by CYP76B101. (C) The mass spectra for the product 2 eluting at 21.1 min. (D) The mass spectra for the product 3 eluting at 21.6 min.
Expression profile of shikonin resistant K562 cell line
GEO Series GSE34298. Homo sapiens. 6 samples. Type: Expression profiling by array.
Effects of shikonin on the gene expression of human lymphoma U937 cells
GEO Series GSE24743. Homo sapiens. 4 samples. Type: Expression profiling by array.
Transcriptome and microRNA array analyses reveal a stimulatory effect of the phytochemical shikonin on epithelial–mesenchymal transition (EMT) in mouse skin
GEO Series GSE32696. Mus musculus. 12 samples. Type: Expression profiling by array; Non-coding RNA profiling by array.
Beta,Beta-Dimethylacryloyl shikonin, mechanism of preventing and treating zebrafish soybean meal enteritis
GEO Series GSE273916. Danio rerio. 9 samples. Type: Expression profiling by high throughput sequencing.
Shikonin inhibits the malignant progression of triple-negative breast cancer
GEO Series GSE281168. Homo sapiens. 6 samples. Type: Expression profiling by high throughput sequencing.
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