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15 results for “soybean aphid”
Improved genome assembly and annotation of the soybean aphid (Aphis glycines Matsumura)
<p>Updated genome assembly and annotation of <em>Aphis glycines</em> biotype 4.</p> <p><strong>Overview of files included in this release:</strong></p> <p><strong>Frozen release:</strong></p> <p>Updated <em>A. glycines </em>biotype 4 genome assembly: Aphis_glycines_4.v2.1.scaffolds.fa.gz </p> <p>BRAKER2 gene models for updated <em>A. glycines </em>biotype 4 genome assembly: Aphis_glycines_4.v2.1.scaffolds.fa.gff</p> <p>BRAKER2 protein sequences: Aphis_glycines_4.v2.1.scaffolds.fa.gff.aa.fa</p> <p>BRAKER2 nucleotide coding sequences: Aphis_glycines_4.v2.1.scaffolds.fa.gff.CDS.fa</p> <p><strong>Unfiltered raw intermediate genome assemblies:</strong></p> <p>Canu assembly of biotype 4 PacBio data from Wenger et. al. (2017): canu.fa.gz</p> <p>DBG2OLC hybrid assembly of selected biotype 4 MiSeq data and biotype 4 PacBio data from Wenger et. al. (2017): DBG2OLC.fa.gz</p> <p>Merged Canu and DBG2OLC assembly created with quickmerge: quickmerge.fa.gz</p> <p>Pilon polished (2 rounds) quickmerge assembly: quickmerge.pilon_r2.fa.gz</p> <p><strong>Mitochondrial and endosymbiont contigs extracted from the pilon polished quickmerge assembly: </strong></p> <p><em>A. glycines </em>biotype 4 mitochondrial genome: Aphis_glycines_4_Buchnera_v1.fa</p> <p><em>A. glycines </em>biotype 4 <em>Buchnera aphidicola</em> contigs: Aphis_glycines_4_Buchnera_v1.fa</p> <p><em>A. glycines </em>biotype 4 <em>Wolbachia</em> contigs: Aphis_glycines_4_Buchnera_v1.fa</p> <p><strong>Other files:</strong></p> <p>MUSCLE alignment of <em>A. glycines </em>v1, <em>A. glycines </em>biotype 4 v2.1 and <em>Drosophila melanogaster</em> R6.22 Osiris proteins in fasta format: D_mel_v1_v2_osiris.prots.muscle.fasta</p> <p>FastTree Maximum Likelihood phylogeny based on the MUSCLE alignment of Osiris genes in newick format: D_mel_v1_v2_osiris.prots.muscle.FastTree.nwk</p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p> <p> </p>
Figure 1. Plant tissue-culture growth chamber Percival. A in Survivorship of soybean aphid biotypes (Hemiptera: Aphididae) on winter hosts, common and glossy buckthorn
Figure 1. Plant tissue-culture growth chamber Percival. A) Soybean plants maintained in a plant growth chamber for 21 days before placed Rhamnus cathartica. B) Leaf of R. cathartica infested with soybean aphid biotype 1. C) Leaf of Frangula alnus with soybean aphid biotype 4.
Figure 3 in Survivorship of soybean aphid biotypes (Hemiptera: Aphididae) on winter hosts, common and glossy buckthorn
Figure 3. Males of soybean aphid, Aphis glycines, biotype 3. A) Alate male. B) Apterous male with sclerites on thorax. C) Apterous male without sclerites on thorax. The slides mounted images were magnified to 64.3x.
Figure 2 in Survivorship of soybean aphid biotypes (Hemiptera: Aphididae) on winter hosts, common and glossy buckthorn
Figure 2. Adult morphs and eggs of soybean aphid, Aphis glycines, biotype 3 on Rhamnus cathartica. A) Gynopara. B) Ovipara. C) Dorsal view of apterous male. D) Ventral view of apterous male. E) Eggs on bud.
Local plant diversity and soybean biological control 2012 Aphid Surveys:Biodiversity II: Effects of Plant Biodiversity on Population and Ecosystem Processes
Biodiversity II (E120) is designed to determine how the number of plant species affects the dynamics of ecological processes at the population, community, and ecosystem levels. By experimentally manipulating the number of species and the kinds of species, the amount of plant growth and the change from year to year, that result can be examined. Plots are large (9m x 9m actively maintained) and well-replicated, allowing responses of plant pathogens, insect herbivores, seed predators, soil parameters, invasive plant species and other variables to also be studied. Plots were seeded in May 1994 to have 1, 2, 4, 8, or 16 species, with roughly 30 replicates of each diversity level. The species composition of each plot was chosen by random draw from a pool of 18 grassland perennials that included four warm-season (C4) grasses, four cool-season (C3) grasses, four legumes, four non-legume forbs, and two woody species. All species occur in monoculture allowing comparison of responses of each species in monoculture to combinations of these same species. The experiment was established in 1994 by the lead investigators David Tilman, Peter Reich, Johannes Knops, and David Wedin. Experiment 120 is similar to Experiment 123, but it uses larger plots to provide a large capacity for long-term subexperiments.
Local plant diversity and soybean biological control 2011 Aphid and Enemy Surveys:Biodiversity II: Effects of Plant Biodiversity on Population and Ecosystem Processes
Biodiversity II (E120) is designed to determine how the number of plant species affects the dynamics of ecological processes at the population, community, and ecosystem levels. By experimentally manipulating the number of species and the kinds of species, the amount of plant growth and the change from year to year, that result can be examined. Plots are large (9m x 9m actively maintained) and well-replicated, allowing responses of plant pathogens, insect herbivores, seed predators, soil parameters, invasive plant species and other variables to also be studied. Plots were seeded in May 1994 to have 1, 2, 4, 8, or 16 species, with roughly 30 replicates of each diversity level. The species composition of each plot was chosen by random draw from a pool of 18 grassland perennials that included four warm-season (C4) grasses, four cool-season (C3) grasses, four legumes, four non-legume forbs, and two woody species. All species occur in monoculture allowing comparison of responses of each species in monoculture to combinations of these same species. The experiment was established in 1994 by the lead investigators David Tilman, Peter Reich, Johannes Knops, and David Wedin. Experiment 120 is similar to Experiment 123, but it uses larger plots to provide a large capacity for long-term subexperiments.
Data from: Implementing an evolutionary framework for understanding genetic relationships of phenotypically defined insect biotypes in the invasive soybean aphid (Aphis glycines)
Adaptive evolution of pest insects in response to the introduction of resistant cultivars is well documented and commonly results in virulent (i.e. capable of feeding upon resistant cultivars) insect populations being labeled as distinct biotypes. Phenotypically defined, biotypes frequently remain evolutionarily indistinct, resulting in ineffective application of virulence control measures and shorter durability of resistant cultivars. Here we utilize an evolutionary framework to discern the genetic relationship between biotypes of the soybean aphid (Aphis glycines, Matsumura). The soybean aphid is invasive in North America, and is among the most destructive pests of commercial soybean on the continent. Attempts to breed host-plant resistant soybean have been hampered by the emergence of virulent aphid biotypes that are unaffected by the plant's resistance mechanism(s). Comparative population genetic analysis of virulent and avirulent (i.e. unable to feed on resistant cultivars) biotypes found populations to be genetically indistinguishable across biotype and geographic distance, with high rates of inter-population immigration and admixture. The lack of genetic distinction between biotypes coupled with elevated genotypic diversity within all populations suggested virulence has a non-genetic based or includes a gene complex that is widely distributed throughout soybean aphid populations, which undergo regular dispersal and unimpeded sexual recombination.
Data from: Implementing an evolutionary framework for understanding genetic relationships of phenotypically defined insect biotypes in the invasive soybean aphid (Aphis glycines)
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Characterization of induced susceptibility effects on soybean- soybean aphid interactions
GEO Series GSE129626. Glycine max. 10 samples. Type: Expression profiling by high throughput sequencing.
Identification of Soybean Genes Differentially Regulated in Near Isogenic Lines Differing in Resistance to Aphid Infestation
GEO Series GSE67779. Glycine max. 20 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome profiling of interaction effects of soybean cyst nematodes and soybean aphids on soybean.
GEO Series GSE125103. Glycine max. 47 samples. Type: Expression profiling by high throughput sequencing.
Data from: Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324
Host-plant resistance is an effective method for controlling soybean aphid (Aphis glycines Matsumura), the most damaging insect pest of soybean (Glycine max (L.) Merr.) in North America. Recently, resistant soybean lines have been discovered and at least four aphid resistance genes (Rag1, Rag2, Rag3 and rag4) have been mapped on different soybean chromosomes. However, the evolution of new soybean aphid biotypes capable of defeating host-plant resistance conferred by most single genes demonstrates the need for finding germplasm with multigenic resistance to the aphid. This study was conducted to map quantitative trait loci (QTL) for aphid resistance in PI 567324. We identified two major QTL (QTL_13_1 and QTL_13_2) for aphid resistance on soybean chromosome 13 using 184 recombinant inbred lines from a 'Wyandot' × PI 567324 cross. QTL_13_1 was located close to the previously reported Rag2 gene locus, and QTL_13_2 was close to the rag4 locus. A minor QTL (QTL_6_1) was also detected on chromosome 6, where no gene for soybean aphid resistance has been reported so far. These results indicate that PI 567324 possesses oligogenic resistance to the soybean aphid. The molecular markers closely linked to the QTL reported here will be useful for development of cultivars with oligogenic resistance that are expected to provide broader and more durable resistance against soybean aphids compared with cultivars with monogenic resistance.
Data from: Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324
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Transcriptional response to soybean aphid infestation in susceptible and resistant soybean plants
GEO Series GSE35427. Glycine max. 24 samples. Type: Expression profiling by array.
Transcriptional response to long-term soybean aphid infestation in susceptible and resistant soybean plants
GEO Series GSE115790. Glycine max. 12 samples. Type: Expression profiling by array.
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