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Dataset results
102 results for “spatial imaging”
R Code and Images for Developing a Spatial Concordance Coefficient at Harvard Forest 2010
Concordance correlation coefficients have been developed in a variety of different contexts. This problem has been widely addressed in a non-spatial context, but here we consider a coefficient that for a fixed spatial lag allows the comparison of two spatial sequences (e.g., images). We define a spatial concordance coefficient for second-order stationary processes.
Spatially Resolved Infrared Radiofluorescence (SR IR-RF) Image Data
<p>This dataset contains measurement sequences and data output <br> of spatially resolved infrared radiofluorescence (SR IR-RF) measurements<br> on K-feldspar samples carried out at the IRAMAT-CRP2A, UMR 5060, CNRS-Université Bordeaux Montaigne (France)<br> in 2019. The data analysis was performed in 2020. </p> <p>The data may serve as reference data and allow detailed inspection by others to <br> verify or advance the used analysis procedures. </p> <p>Along with the raw image data (TIF-files), the datasets also contain documented R scripts used for data processing and partly treated data as an example. To reproduce the full data analysis, additional software is needed; not part of this repository. </p> <p>Further details can be found in the README.md (README.html), which is part of the dataset.</p>
Vectra Polatis image of human colorectal cancer (CRC1) from: A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially resolved tissue phenotyping at single-cell resolution.
<p>Two 4 µm thick serial sections were cut from CRC1 FFPE block using a microtome. The first slide was dewaxed and rehydrated before carrying out HIER with Antigen Retrieval Reagent-Basic (R&D Systems). The tissue was then blocked and incubated with the anti-CD3 antibody (Dako, Supplementary Table 2) followed by horseradish peroxidase (HRP) conjugated anti-rabbit antibody (Dako) and stained with 3,3' diaminobenzidine (DAB) substrate (Abcam) and haematoxylin. Areas with CD3<sup>+</sup> infiltration in the proximity of the tumour invasive margin were identified by a clinical pathologist (M. R-J.)</p> <p>The second slide was stained with a panel of six antibodies (CD8, PD1, Ki67, PDL1, CD68, GzB, Supplementary Table 2), Opal fluorophores and 4’,6-diamidino-2-phenylindole (DAPI) on a Ventana Discovery Ultra automated staining platform (Roche). Expected expression and cellular localisation of each marker as well as fluorophore brightness were used to minimise fluorescence spillage upon antibody-Opal pairing. Following a one-hour incubation at a 60°C, the slide was subjected to an automated staining protocol on an autostainer. The protocol involved deparaffinisation (EZ-Prep solution, Roche), HIER (DISC. CC1 solution, Roche) and seven sequential rounds of: one hour incubation with the primary antibody, 12 minutes incubation with the HRP-conjugated secondary antibody (DISC. Omnimap anti-Ms HRP RUO or DISC. Omnimap anti-Rb HRP RUO, Roche) and 16 minute incubation with the Opal reactive fluorophore (Akoya Biosciences). For the last round of staining, the slide was incubated with Opal TSA-DIG reagent (Akoya Biosciences) for 12 minutes followed by Opal 780 reactive fluorophore for our hour (Akoya Biosciences). A denaturation step (100°C for 8 minutes) was introduced between each staining round in order to remove the primary and secondary antibodies from the previous cycle without disrupting the fluorescent signal. The slide was counterstained with DAPI (Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific). The Vectra Polaris automated quantitative pathology imaging system (Akoya Biosciences) was used to scan the labelled slide. Six fields of view, within the area selected by the pathologist, were scanned at 20x and 40x magnification using appropriate exposure times and loaded into inForm{Kramer, 2018 #23} for spectral unmixing and autofluorescence isolation using the spectral libraries. After spectral unmixing and merging of six 20x fields of view for a total of >5mm<sup>2</sup> ROI (Table 2), one single-tiff image was extracted for each marker and its intensity was rescaled from 0 to 1 with custom R scripts.</p>
LungVis1.0: Active learning AI-powered 3D imaging ecosystem for spatial profiling of lung geometry and pulmonary nanoparticle delivery
<p>The imaging dataset was obtained by light sheet fluorescence microscopy on tissue cleared murine lungs. It includes whole lung autofluorence image, particle fluorescence image, and artifical intelligence nnU-Net generated lung airway segments. The dataset provides 78 healthy murine lung strucutre and airway geometry for C57BL/6 mice and offers comprehensive delivery features including qualitative and quantitative analysis on the temporal and spatial inter- and intra-acinar deposition patterns and NP regional dosimetry for four commonly-used routes of pulmonary delivery,namely intranasal liquid aspiration, intratracheal liquid instillation, ventilator-assisted and nose-only aerosol inhalation.</p> <p>Raw LSFM imaging data collection was carried out between 2017-2021, the AI code and generated airway segmention were performed in 2021-2022, the whole datasets were then compiled in 2023. </p> <p>Please ensure to cite our paper for any reuse or reanalysis. Yang, L., Liu, Q., Kumar, P. <em>et al.</em> LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages. <em>Nat Commun</em> <strong>15</strong>, 10138 (2024). https://doi.org/10.1038/s41467-024-54267-1</p> <p>For any inquiries, please feel free to contact us at lin.yang@helmholtz-munich.de </p>
Multigrid spatially constrained dispersion curve inversion package: towards distributed acoustic sensing surface wave imaging
<p>Surface wave methods, commonly applied in diverse fields, encounter challenges in complex subsurface environments due to limitations inherent in traditional inversion techniques. Conventional one-dimensional inversion (1DI), with its reliance on fixed grids and deterministic linear approaches, often introduces biases, diminishing lateral resolution. Laterally constrained inversion (LCI) improves robustness by addressing lateral coherency but falls short in delineating arbitrary interfaces due to its dependency on fixed grid models. The advent of Distributed Acoustic Sensing (DAS) technology offers extensive seismic data, yet its potential for high-resolution imaging remains underutilized. We introduce a Multigrid Spatially Constrained Dispersion Curve Inversion (MCI) method to overcome these challenges, aiming to harness high-resolution DAS surface wave imaging capabilities. </p> <p>The package includes essential scripts and models required to replicate key figures from the study by Guan et al. (2023, currently under review). These codes are designed to help readers evaluate the effectiveness of the MCI approach using synthetic demonstrations. Additionally, the package includes a refined 2D Vs (shear wave velocity) model derived from a DAS (Distributed Acoustic Sensing) field study conducted in Imperial Valley, California. This model offers new insights into the regional fault system, underscoring the importance of enhanced spatial resolution in large-scale geophysical investigations.</p> <p>It is organized into three directories and contains a total of 14 files. The directory structure is as follows:<br>├── DAS field data<br>│ ├── Pltmodels.m<br>│ ├── README.txt<br>│ ├── field_models.pdf<br>│ ├── model_1DI.mat<br>│ ├── model_LCI.mat<br>│ └── model_MCI.mat<br>├── MCI_Main<br>│ ├── DisForward.p<br>│ ├── InvForward.p<br>│ ├── InvJacobian.p<br>│ ├── MCI.p<br>│ ├── readme.txt<br>│ └── whitejet3.m<br>└── Synthetic demos<br> ├── MCI_Main.m<br> └── syndata.mat</p>
Accompanying dataset for: "IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues"
<p>Mouse datasets were acquired using the manual IBEX multiplex imaging protocol and accompany the manuscript “IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues”, A. Radtke <em>et al.</em>, 2020, PNAS.</p> <p>All image data are stored using the <a href="https://imaris.oxinst.com/support/imaris-file-format">Imaris file format</a>. To view these multi-channel images, you can either use one of these free viewers, <a href="https://imaris.oxinst.com/imaris-viewer">Imaris viewer</a>, <a href="https://imagej.net/Fiji">Fiji</a>.</p> <p>Each experiment has an associated imaging meta-data file in xlsx format and the resulting image in Imaris format.</p> <p><strong>Mouse spleen (Manual)</strong></p> <p>Dataset is a 16 parameter IBEX experiment performed on a mouse spleen section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse thymus (Manual)</strong></p> <p>Dataset is a 26 parameter IBEX experiment performed on a mouse thymus section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse lung (Manual)</strong></p> <p>Dataset is a 23 parameter IBEX experiment performed on a mouse lung section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.379 µm), y (0.379 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse small intestine (Manual)</strong></p> <p>Dataset is a 20 parameter IBEX experiment performed on a mouse small intestine section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse liver (Manual)</strong></p> <p>Dataset is an 18 parameter IBEX experiment performed on a liver section from a LysM-tdtomato reporter mouse labeled with antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse naive lymph node (Manual)</strong></p> <p>Dataset is a 41 parameter IBEX experiment performed on a mouse lymph node section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p> <p><strong>Mouse immunized lymph node (Manual)</strong></p> <p>Dataset is a 41 parameter IBEX experiment performed on a mouse lymph node section labeled with the nuclear marker JOJO-1 and antibodies directed against the indicated markers. Images were acquired using an inverted Leica TCS SP8 X confocal microscope equipped with a 40X objective (NA 1.3), 4 HyD and 1 PMT detectors, a white light laser that produces a continuous spectral output between 470 and 670 nm as well as 405, 685, and 730 nm lasers. All images were captured at an 8-bit depth, with a line average of 3, and 1024x1024 format with the following pixel dimensions: x (0.284 µm), y (0.284 µm), and z (1 µm). Images were tiled and merged using the LAS X Navigator software (LAS X 3.5.5.19976).</p>
Code and data for: Decoupling channel count from field-of-view and spatial resolution in single-sensor imaging systems for fluorescence image-guided surgery
<p><em>Significance</em></p> <p>Near-infrared fluorescence image-guided surgery is often thought of as a spectral imaging problem where the channel count is the critical parameter, but it should also be thought of as a multiscale imaging problem where the field-of-view and spatial resolution are similarly important.</p> <p><em>Aim</em></p> <p>Conventional imaging systems based on division-of-focal-plane architectures suffer from a strict relationship between the channel count on one hand and the field-of-view and spatial resolution on the other, but bioinspired imaging systems that combine stacked photodiode image sensors and long-pass/short-pass filter arrays offer a weaker tradeoff.</p> <p><em>Approach</em></p> <p>In this paper, we explore how the relevant changes to the image sensor and associated image processing routines affect image fidelity during image-guided surgeries for tumor removal in an animal model of breast cancer and nodal mapping in women with breast cancer.</p> <p><em>Results</em></p> <p>We demonstrate that a transition from a conventional imaging system to a bioinspired one, along with optimization of the image processing routines, yields improvements in multiple measures of spectral and textural rendition relevant to surgical decision-making.</p> <p><em>Conclusions</em></p> <p>These results call for a critical examination of the devices and algorithms that underpin image-guided surgery to ensure that surgeons receive high-quality guidance and patients receive high-quality outcomes as these technologies enter clinical practice.</p>
Imaging Mass Cytometry Images (APP1) from: A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially resolved tissue phenotyping at single-cell resolution.
<p>Four µm-thick sections were cut from the APP1 FFPE block with a microtome and used for staining with a panel of 26 antibodies targeting the main immune, stromal and epithelial cell populations of the gastrointestinal tract (Supplementary Table 2). The optimal dilution of each antibody in the panel was identified by staining and ablating FFPE appendix sections. The resulting images were reviewed by a mucosal immunologist (J.S.) and the dilution giving the best signal to background ratio was selected for each antibody (Supplementary Table 2). To perform the staining for IMC, slides were dewaxed after a one-hour incubation at 60°C, rehydrated and heat-induced antigen retrieval was performed with a pressure cooker in Antigen Retrieval Reagent-Basic (R&D Systems). Slides were incubated in a 10% BSA (Sigma), 0.1% Tween (Sigma), and 2% Kiovig (Shire Pharmaceuticals) Superblock Blocking Buffer (Thermo Fisher) blocking solution at room temperature for two hours. Each antibody was added to a primary antibody mix at the selected concentration in blocking solution and incubated overnight at 4°C. After two washes in PBS and PBS-0.1% Tween, the slides were treated with the DNA intercalator Cell-ID™ Intercalator-Ir (Fluidigm) (containing the two iridium isotopes 191Ir and 193Ir) 1.25 mM in a PBS solution. After a 30-minute incubation, the slides were washed once in PBS and once in MilliQ water and air-dried. The stained slides were then loaded in the Hyperion Imaging System (Fluidigm) imaging module to obtain light-contrast high resolution images of approximately four mm<sup>2</sup>. These images were used to select the ROI in each slide. For APP1, a one mm<sup>2</sup> ROI containing a lymphoid follicle in its whole depth alongside a portion of lamina propria and of epithelium was selected. ROIs were ablated at a o µm/pixel resolution and 200 Hz frequency.</p>
Data for 'Deriving spatial features from in situ proteomics imaging to enhance cancer survival analysis'
<p>Additional data for 'Deriving spatial features from in situ proteomics imaging to enhance cancer survival analysis'</p>
scProAtlas: an atlas of multiplexed single-cell spatial proteomics imaging in human tissues
<p>All analysis results for the spatial proteomics imaging techniques in the scProAtlas database are stored in compressed files named accordingly. Within each compressed file, the folders are organized in a fixed storage structure in the following order: Analysis module > Imaging Technique > Dataset > Tissue > ROI.</p> <p>Each folder contains the corresponding metadata (including original sample information, cell type annotations, and neighborhood annotations) stored in a file named <code>cells.tsv</code>. Additionally, the module used to identify spatial pattern genes includes an <code>anndata</code> format file, named <code>adata_moran.h5ad</code>, which stores the integrated results of scRNA-seq and spatial proteomics.</p> <p>scProAtlas_analysis_code.tar.gz contains example codes for all analysis modules in scProAtlas. Here, we provide the example using <strong>SCP_CODEX1 - Large intestine. </strong>The codes include all the scripts used for the entire workflow, from image segmentation to scRNA-spatial proteomics integration, and spatial analysis.</p> <p>We have also uploaded the raw protein channel matrices with AnnData format in <strong>version 3 and 4.</strong></p>
Code and data for: Decoupling channel count from field-of-view and spatial resolution in single-sensor imaging systems for fluorescence image-guided surgery
Open the record for dataset details and reuse information.
Data package from "Regional Mapping and Spatial Distribution Analysis of Canopy Palms in an Amazon Forest Using Deep Learning and VHR Images"
<p>This data package contains the very high resolution maps of canopy palms from the paper "Regional Mapping and Spatial Distribution Analysis of Canopy Palms in an Amazon Forest Using Deep Learning and VHR Images". These maps have been produced with two GeoEye-1 very high resolution images (0.5 m) and a Deep Learning method for image segmentation called U-net, methods and data are fully described in the article. The total size of the decompressed archive is 2.56 Go and is distributed in two shapefiles, one for each GeoEye-1 image. When using this dataset, please cite the original article https://doi.org/10.3390/rs12142225</p>
Regulatory T cell therapy is associated with distinct immune regulatory lymphocytic infiltrates in kidney transplants: Spatial transcriptomic dataset and images
<p>The outputs of the NanoString GeoMx DSP platform were concatenated into three xlsx files, each illustrating a separate experiment along with their sample annotations. This technique analyzes protein or RNA abundance within regions of interest (ROIs) or specific cell segments selected based on histological features and immunofluorescence. In this repository, the concatenated GeoMx output files are presented, along with PowerPoint presentations for each biopsy that show immunofluorescence images of the selected ROIs and/or cell segments.</p> <ul> <li><strong>Protein_Full ROI:</strong> This experiment measured the abundance of 41 proteins in discrete regions of interest (ROIs) within transplant kidney biopsies.</li> <li><strong>Protein_Rare cell:</strong> This experiment measured the abundance of 40 proteins in specific cell segments, such as CD4+FoxP3- cells vs. CD4+FoxP3+ cells, within transplant kidney biopsies.</li> <li><strong>RNA:</strong> This experiment measured the abundance of 90 genes in discrete ROIs within transplant kidney biopsies.</li> </ul>
Lublin 1944 aerial images with spatial overlay index
<p><em><strong>1. Lublin 1944 aerial image overlay index</strong></em> [.geojson or .gml file] is a vectorized, digital form of selected overlay indexes for degree square 51N022E (https://catalog.archives.gov/id/44241929) of German Flown Aerial Photographs,1939-1945 (https://catalog.archives.gov/id/306065) archived in National Archives and Records Administration, College Park, MD.</p> <p>2. The vectorized index contains geometries, attributes and other metadata of 104 aerial images of Lublin [Poland] captured by Luftwaffe reconaissance from 10th May 1944 to 6th December 1944.</p> <p>3. The dataset contains the archive of 104 digital copies of aerial images, scaned with A2-3050-Sharp363N. The images are .jpg files with 24-bit colour depth and resolution 600 dpi. File size: from 7,5 MB to 20 MB.</p> <p>4. The mosaic of aerial images is uploaded in Ortofotomapa_1944_modificado_3.tif - 0,8GB file. TFW, AUX and OVR files added for GIS users. TPK file with ESRI tiled package is added. This is also available via spatial data services (TMS and WMTS):</p> <p>- XYZ/TMS layers available at <strong><em>//ortolub.umcs.pl/data/tiles_3857/{z}/{x}/{y}.png </em></strong>or via https://ortolub.umcs.pl/map_en.html</p> <p>- WMTS layer available at <strong><em>//tiles.arcgis.com/tiles/STaxETJ8DGWEoQ8D/arcgis/rest/services/Ortolub_1944/MapServer </em></strong>or via ArcGIS Online https://www.arcgis.com/home/item.html?id=d5dd97b49b014d6ea0e6bc221fc37668</p> <p>5. The mosaic cropped to 1931-1947 city boundaries are added: Lublin_1944_aerial_10k_600dpi_gsc.jpg and Lublin_1944_aerial_adm_10k_600dpi_gsc.jpg with area outside the boundaries masked. This is also available at Wikimedia Commons, https://commons.wikimedia.org/wiki/File:Lublin_1944_aerial_image.jpg</p> <p>6. The project and the platform <em><strong>https://ortolub.umcs.pl</strong></em> was developed under the Polish National Science Centre grant programme - Miniatura 4.0. ref. no. 2020/04/X/HS4/00382. I hereby share my work under Creative Commons license CC BY-SA 4.0 (Attribution - ShareAlike).</p>
Supporting data for boundary layer water vapour statistics from high-spatial-resolution spaceborne imaging spectroscopy
<p>This dataset includes the properties necessary to reproduce the analysis of water vapour statistics derived from imaging spectroscopy as in:</p> <p>Richardson et al. (2021a) DOI: 10.5194/amt-14-5555-2021<br> Richardson et al. (2021b) DOI: 10.5194/amt-2021-163 (pre-acceptance DOI, follow links to published version)</p> <p>Files include the retrieval emulator parameters, atmospheric profiles used in the emulator development, column-mean water vapour and cloud water both for the total column water vapour (TCWV) and "effective" TCWV, which accounts for the water vapour integrated along the direct solar path at a range of solar zenith angles, see Richardson 2021b, Eq. (7).</p>
Data and code for "High-speed 3D imaging flow cytometry with optofluidic spatial transformation"
<p>Data and codes used in Ugawa & Ota, "High-speed 3D imaging flow cytometry with optofluidic spatial transformation".</p>
Raw Data - Part 4 : Spatial multi-omic map of human myocardial infarction ---- Raw image
<p>We provide here the raw image for the visium data for the manuscript: Kuppe, Ramirez Flores, Li et al. "Spatial multi-omic map of human myocardial infarction", 2022</p>
Data from: Hyperspectral imaging reveals differential carotenoid and chlorophyll temporal dynamics and spatial patterns in Scots pine under water stress
<p>Data and codes associated with the manuscript '<span>Hyperspectral imaging reveals differential carotenoid and chlorophyll temporal dynamics and spatial patterns in Scots pine under water stress</span>'. </p>
Data Set: Hyperspectral image unmixing with LiDAR data-aided spatial regularization
<p>Data set and matlab codes used for the experimental section of "Hyperspectral Image Unmixing With LiDAR Data-Aided Spatial Regularization"</p> <p>T. Uezato, M. Fauvel and N. Dobigeon, "Hyperspectral Image Unmixing With LiDAR Data-Aided Spatial Regularization," in <em>IEEE Transactions on Geoscience and Remote Sensing</em>, vol. 56, no. 7, pp. 4098-4108, July 2018.<br> doi: 10.1109/TGRS.2018.2823419<br> URL: <a href="http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8347066&isnumber=8393475">http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=8347066&isnumber=8393475</a><br> </p>
Spatial- and Fourier-domain ptychography for high throughput bio-imaging
<p>Title: Spatial and Fourier domain ptychography for high-throughput bio-imaging<br> Version: 1.0 <br> Copyright: Shaowei Jiang, Pengming Song, Guoan Zheng, 2023<br> License: Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License</p> <p>******************************************************************************</p> <p>If you use this code, please cite the following paper:<br> Shaowei Jiang, Pengming Song, Tianbo Wang, Liming Yang,Ruihai Wang, Chengfei Guo, Bin Feng, Andrew Maiden, and Guoan Zheng,<br> "Spatial and Fourier domain ptychography for high-throughput bio-imaging", Nature Protocols, 2023. </p> <p>For algorithmic details, please refer to our paper.</p> <p>******************************************************************************</p> <p>How to use: </p> <p>Foruier-domain ptychography (FP)<br> 1. Unpack the full package and install related softwares. <br> Refer to 'Materials for Procedure 1: Fourier-domain ptychography' and 'Procedure 1: Fourier-domain ptychography' sections in our paper for additional details (including version number and instructions). <br> 2. We provide 4 different FP experimental datasets. Please refer to 'Content in this package' section for additional details. <br> 3. Run FP_Recovery.m for FP image reconstruction. The reconstruction time for a tile of 256x256 dimensions, with 4-times padding, is ~2 seconds. Please refer to our paper for additional information. </p> <p>Spatial-domain coded ptychography (CP)<br> 1. Unpack the full package and install related softwares. <br> Refer to 'Materials for Procedure 2: Spatial-domain coded ptychography' and 'Procedure 2: Spatial-domain coded ptychography' sections in our paper for additional details (including version number and instructions). <br> 2. We provide 3 different CP experimental datasets. Please refer to 'Content in this package' section for additional details. <br> 3. Run CP_Recovery.m for CP image reconstruction. The reconstruction time for raw images with 1024*1024 dimensions, with 3-times padding, is ~58 seconds. Please refer to our paper for additional information. </p> <p>******************************************************************************</p> <p>Content in this package: <br> CP_HeLaCellCulture Dataset and reconstruction code of HeLa cell sample for CP<br> CP_Immunohistochemistry Dataset and reconstruction code of IHC stained sample for CP<br> CP_UnstainedCytologySmear Dataset and reconstruction code of unstained cytology smear for CP<br> FP_Intestine_Aberrations Dataset and reconstruction code of Intestine cancer sample for FP<br> FP_H&E_RGB Dataset (RGB) and reconstruction code of H&E stained sample for FP<br> FP_Immunohistochemistry_RGB Dataset (RGB) and reconstruction code of IHC stained sample for FP<br> FP_Leukemia_RGB Dataset (RGB) and reconstruction code of Leukemia sample for FP</p> <p>******************************************************************************</p> <p>License of using this package (refer to 'license.txt'): </p> <p>Creative Commons Attribution-NonCommercial-ShareAlike 4.0<br> International Public License</p> <p>Any inclusion or other use of this package means acceptance of this license.</p>
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.