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26 results for “speed cells”
Raw data accompanying the manuscript "Cost-effective high-speed, three-dimensional live-cell imaging of HIV-1 transfer at the T cell virological synapse"
<p>These are the raw datasets used to generate the figures for the manuscript entitled "Cost-effective high-speed, three-dimensional live-cell imaging of HIV-1 transfer at the T cell virological synapse". The data files are 3D image stacks of a custom-built wide field deconvolution fluorescence microscope (.tif) and super-resolution structured illumination microscopy data (.dv) of Jurkat T cells transferring HIV-1 virus particles to previously uninfected primary T cells.</p>
Computer code accompanying Schraivogel, D. et al. "High-speed fluorescence image-enabled cell sorting" Science, 2022. doi: 10.1126/science.abj3013
<p>Computer code accompanying Schraivogel et al. "High-speed fluorescence image-enabled cell sorting". Details are provided in the manuscript's data and materials availability section and table 3.</p> <p> </p> <p>We provide three directories:</p> <p>(1) R code to reproduce figures (ICS2021_0.1.0.tar.gz)</p> <p>(2) Python code to reproduce figures (ICS_Fiji_Plugin.zip)</p> <p>(3) Code for ICS/CellView Fiji plugins (ICSPython.zip)</p> <p> </p> <p>Code for (1) and (3) has also been shared via Github:</p> <p>https://github.com/benediktrauscher/ICS</p> <p>https://github.com/embl-cba/ICS</p> <p> </p> <p>We recommend downloading the ICS Fiji plugins via Github or to install them using the Fiji update site to ensure you're using the most recent version.</p>
Effect of heterogeneous substrate adhesivity of follower cells on speed and tension profile of leader cells in primary keratocyte collective cell migration
<p><span>In single keratocyte motility, membrane tension is reported to be high at cell-fronts and believed to establish front coherence. To understand role of membrane mechanics in collective cell migration, we study membrane height fluctuations in cell sheets from fish scales using interference reflection microscopy (IRM). We report the monolayer to have cells lacking substrate adhesion and show that such "non-sticky" cells can form bridges between leader cells and far-away follower cells. Do such interactions alter motility and membrane mechanics in such leaders? We find non-significant, but reduced speed for leaders with "non-sticky" followers in comparison to other leaders. Cells show high phenotypic variability in their membrane fluctuation tension profiles. On average, this tension is found to be lower at cell fronts than the mid-section. However, leaders with non-sticky followers are more prone to display higher tension at their front and have a negative correlation between cell speed and front-mid tension difference. We, thus, conclude that intracellular tension gradients are heterogeneous in cell sheets and substrate adhesivity of followers can control the coupling of the gradient to cell speed.</span></p>
High-speed video microscopy analysis of cilia before and after airway cell culture
<p>High-speed video microscopy analysis (HSVA) is a diagnostic tool used within the UK Primary Ciliary Dyskinesia (PCD) Service to access airway ciliary function on nasal brushing biopsies. The Southampton PCD group is based at the University of Southampton, Faculty of Medicine and the University Hospital Southampton NHS Foundation Trust and is led by Professor Jane Lucas. We also use air-liquid interface (ALI) culture to differentiate airway epithelial cells to regrow healthy cilia to repeat standard PCD tests (including HSVA, immunofluoresence labelling of cilia proteins, transmission electron microscopy and functional genomics) and provide PCD research samples, which also allow us to develop new diagnostic approaches. ALI-culture can restore normal ciliary movement when secondary damage (due to infection or poor cell health) temporarily causes of abnormal cilia movement or a lack of cilia. ALI-culture can also re-confirm when ciliary defects and abnormal ciliary function are permanent and cause by inherited PCD (a ciliopathy). </p>
A look beyond topography: transient phenomena of Escherichia coli cell division captured with high-speed in-line force mapping
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Effect of heterogeneous substrate adhesivity of follower cells on speed and tension profile of leader cells in primary keratocyte collective cell migration
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Data from: Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus
Directional cell migration in dense three-dimensional (3D) environments critically depends upon shape adaptation and is impeded depending on the size and rigidity of the nucleus. Accordingly, the nucleus is primarily understood as a physical obstacle, however, its pro-migratory functions by step-wise deformation and reshaping remain unclear. Using atomic force spectroscopy, time-lapse fluorescence microscopy and shape change analysis tools, we determined nuclear size, deformability, morphology and shape change of HT1080 fibrosarcoma cells expressing the Fucci cell cycle indicator or being pre-treated with chromatin-decondensating agent TSA. We show oscillating peak accelerations during migration through 3D collagen matrices and microdevices that occur during shape reversion of deformed nuclei (recoil), and increase with confinement. During G1 cell cycle phase, nucleus stiffness was increased and yielded further increased speed fluctuations together with sustained cell migration rates in confinement as compared to interphase populations, or to periods of intrinsic nuclear softening in the S/G2 cell cycle phase. Likewise, nuclear softening by pharmacological chromatin decondensation or after lamin A/C depletion reduced peak oscillations in confinement. In conclusion, deformation and recoil of the stiff nucleus contributes to saltatory locomotion in dense tissues.
Data from: Positive size-speed relationships in gametes and vegetative cells of Chlamydomonas reinhardtii; implications for the evolution of sperm.
It is commonly held that differences in gametes of the two sexes (anisogamy) evolved from ancestors whose gametes were similar in size and behaviour (isogamy). Underlying many hypotheses explaining anisogamy are assumed relationships between cell size and speed in the ancestral isogamous population. Using the isogamous alga Chlamydomonas reinhardtii, we explored size-speed distributions in vegetative and gamete cells of ten cell lines, and clonal data from within two cell lines. We applied an independent speed selection approach to gamete populations of C. reinhardtii, monitoring correlated responses in size following selection for high speed. We demonstrate positive size-speed relationships in clones, cell lines and artificially selected speed selection lines. We found different size-speed relationships in the two cell types of C. reinhardtii even though they overlap in size, suggesting that cell composition and/or programs of gene expression are capable of altering this relationship, and that the relationship is evolvable. The positive genetic size-speed correlation means that the division of parent vegetative cells into numerous gametes trades off against not only size but also speed, a trade-off that has not received previous attention. Our results support re-evaluating the role of speed selection in the evolution of anisogamy.
Data from: Positive size-speed relationships in gametes and vegetative cells of Chlamydomonas reinhardtii; implications for the evolution of sperm.
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Data from: Cell migration through three-dimensional confining pores: speed accelerations by deformation and recoil of the nucleus
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Transcriptome analysis of cells of different cycling speed during Yamanaka reprogramming
GEO Series GSE53074. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
Quantifying DNA replication speeds in single cells by scEdU-seq
GEO Series GSE211037. Homo sapiens. 50 samples. Type: Other.
High-speed live cell interferometry for screening bioprinted organoids
GEO Series GSE218693. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
Arginine methylation of C/EBP⍺ controls the speed of immune cell transdifferentiation (RNA-Seq)
GEO Series GSE204745. Mus musculus. 36 samples. Type: Expression profiling by high throughput sequencing.
High-speed fluorescence image-enabled cell sorting
GEO Series GSE167944. Homo sapiens. 39 samples. Type: Other.
Genome-wide and experimental resolution of relative translation elongation speed at individual gene level in human cells
GEO Series GSE46613. Homo sapiens. 7 samples. Type: Expression profiling by high throughput sequencing; Other.
Statistical inference with a manifold-constrained RNA velocity model uncovers cell cycle speed modulations
GEO Series GSE250148. Homo sapiens. 3 samples. Type: Expression profiling by high throughput sequencing.
Arginine methylation of C/EBP⍺ controls the speed of immune cell transdifferentiation (ATAC-Seq)
GEO Series GSE204742. Mus musculus. 36 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Arginine methylation of C/EBP⍺ controls the speed of immune cell transdifferentiation
GEO Series GSE204746. Mus musculus. 80 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Arginine methylation of C/EBP⍺ controls the speed of immune cell transdifferentiation (ChIP-Seq)
GEO Series GSE229072. Mus musculus. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.