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18 results for “succinate dehydrogenase”

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zenodo40/100

Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis

<p>Data supporting results published by Ricci et al. Pyruvate transamination and NAD biosynthesis enable proliferation of succinate dehydrogenase-deficient cells by supporting aerobic glycolysis. Cell Death and Disease (2023) 14:403 (https://doi.org/10.1038/s41419-023-05927-5).</p>

opencc-by-4.0Jul 2024View details →
zenodo40/100

Global effects of deletion of the sdh genes, encoding succinate dehydrogenase, and of cobalt on protein abundance in stationary phase Salmonella enterica serovar Typhimurium.

<p>A common strategy that bacteria utilize to increase their survival under stressful conditions in their natural environments, including antibiotic treatment, is the entry into quiescence, a state of reversible cell growth arrest that offers protection against many environmental insults. Understanding quiescence is an important fundamental question, with relevance in the medical and environmental fields. Little is known about the molecular and physiological determinants that orchestrate survival during this temporary arrest of proliferation, or those that allow a rapid transition back to the proliferating state when conditions again become favorable. In the wide host-range pathogen <em>Salmonella enterica</em> serovar Typhimurium (S. Typhimurium) and other Gram-negative bacteria, this temporary arrest of proliferation induces the expression of the alternative sigma subunit of RNA polymerase, &sigma;S/RpoS, which remodels global gene expression to reshape the cell physiology and ensure survival under starvation and various stress conditions (<em>i.e</em>., the general stress response). One important aspect of persistence is the phenotypic differentiation of quiescent populations into sub-population(s) of "persisters" that survive in the presence of lethal concentrations of antibiotics. This phenomenon is worsening the worldwide antibiotic crisis, by causing therapy failure and chronic infections and potentially favoring the development of antibiotic resistance. Understanding mechanisms governing bacterial persisters is thus an important topic and a key issue for drug developments. However, despites many studies, the physiological and molecular mechanisms controlling the formation of persisters are poorly understood and controversial.</p> <p>We have recently discovered an unexpected functional interaction between &sigma;S and succinate dehydrogenase (Sdh) in the formation of persisters. Succinate dehydrogenase (Sdh), a membrane bound complex that connects the TCA cycle and respiratory chain, is one major target down regulated by &sigma;S (Levi-Meyrueis <em>et al</em>. 2014, 2015, Lago <em>et al.</em> 2017). Stationary-phase <em>Salmonella</em> grown in LB rich medium form persisters with a higher frequency than actively growing bacteria, after transfer to fresh LB medium in the presence of lethal concentrations of ampicillin and ciprofloxacin, but not significant effect of the &Delta;<em>rpoS</em> mutation on this phenomenon was observed. Surprisingly however, the &Delta;<em>rpoS</em> mutation suppressed the defect in persister formation of a &Delta;<em>sdh </em>mutant. It is very likely that the &Delta;<em>rpoS</em> mutation compensates for a metabolic perturbation provoked by the &Delta;<em>sdh </em>mutation, and key for persister formation.</p> <p>To get further insights into the synthetic rescue process involved in persisters formation, we used a mass spectrometry-based proteomics approach to compare the proteome of the wild-type, &Delta;<em>rpoS</em>, &Delta;<em>sdh</em> and &Delta;<em>sdh</em>&Delta;<em>rpoS</em> strains grown to late stationary phase in nutrient-rich LB medium. Cells were also grown in LB supplemented with cobalt to pinpoint major changes induced by cobalt on the <em>Salmonella</em> proteome. Indeed, the synthetic rescue process involved in persisters formation in the presence of ampicillin was abolished when the inoculum has been grown in the presence of a non-lethal dose of cobalt (100 &mu;M).</p> <p><strong>Accession number</strong>.The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier <strong>PXD043726.</strong></p> <p><strong>See also:</strong></p> <p>NOREL, F., &amp; MONTEIL, V. (2023). Unraveling a synthetic rescue process involved in persisters formation [Data set]. Zenodo. https://doi.org/10.5281/zenodo.10277562</p> <p>Ph&eacute;gnon, L., Uttenweiler-Joseph, S., &amp; L&eacute;tisse, F. (2024). <span>Key physiological and metabolic characteristics for the differentiation of quiescent Salmonella's cells into persisters [Data set]. </span>Zenodo. <a href="https://doi.org/10.5281/zenodo.10885905" target="_blank" rel="noopener">https://doi.org/10.5281/zenodo.10885905</a></p> <p><strong>This work was supported by the French National Research Agency (ANR-19-CE44-0005-01, PERIOMET project).</strong></p> <p><strong>References</strong></p> <p>Levi-Meyrueis C, Monteil V, Sismeiro O, Dillies MA, Monot M, Jagla B<em>, et al. </em>Expanding the RpoS/sigmaS-network by RNA sequencing and identification of sigmaS-controlled small RNAs in <em>Salmonella</em>. PloS one. 2014;9(5):e96918.</p> <p>Levi-Meyrueis C, Monteil V, Sismeiro O, Dillies M-A, Kolb A, Monot M <em>et al</em>. Repressor activity of the RpoS/sigmaS-dependent RNA polymerase requires DNA binding. Nucleic Acids Res 2015 43, 1456&ndash;1468.</p> <p>Lago M, Monteil V, Douche T, Guglielmini J, Criscuolo A, Maufrais C, <em>et al</em>. Proteome remodelling by the stress sigma factor RpoS/sigma(S) in <em>Salmonella</em>: identification of small proteins and evidence for post-transcriptional regulation. Scientific reports. 2017;7(1):2127.</p> <p>&nbsp;</p>

opencc-by-4.0Aug 2023View details →
ClinicalTrials.gov36/100

A Phase II Trial of the DNA Methyl Transferase Inhibitor, Guadecitabine (SGI-110), in Children and Adults With Wild Type GIST,Pheochromocytoma and Paraganglioma Associated With Succinate Dehydrogenase

ClinicalTrials.gov study NCT03165721. IPD Sharing: NO. Countries: 1. Publications: 1.

closedIPD-NOFeb 2026View details →
ClinicalTrials.gov36/100

Phase 2 Clinical Trial of SGS-742 Therapy in Succinic Semialdehyde Dehydrogenase Deficiency

ClinicalTrials.gov study NCT02019667. IPD Sharing: Not stated. Countries: 1. Publications: 4.

restrictedIPD-UNDECIDEDFeb 2026View details →
dryad36/100

Itaconate drives mtRNA-mediated Type I interferon production via inhibition of succinate dehydrogenase

Open the record for dataset details and reuse information.

publicSep 2024View details →
ClinicalTrials.gov32/100

Relationship Between Succinate Dehydrogenase Mutations and High-Altitude Illness

ClinicalTrials.gov study NCT00202683. IPD Sharing: Not stated. Countries: 1. Publications: 3.

restrictedIPD-UNDECIDEDFeb 2026View details →
ClinicalTrials.gov32/100

Natural History Study of Patients With Succinic Semialdehyde Dehydrogenase (SSADH) Deficiency

ClinicalTrials.gov study NCT03758521. IPD Sharing: Not stated. Countries: 4. Publications: 2.

restrictedIPD-UNDECIDEDFeb 2026View details →
ClinicalTrials.gov28/100

Brain Excitability in Patients With Succinic Semialdehyde Dehydrogenase Deficiency

ClinicalTrials.gov study NCT00132366. IPD Sharing: Not stated. Countries: 1. Publications: 3.

restrictedIPD-UNDECIDEDFeb 2026View details →
ClinicalTrials.gov28/100

PET Imaging of GABA Receptors in Succinic Semialdehyde Dehydrogenase Deficiency

ClinicalTrials.gov study NCT00246870. IPD Sharing: Not stated. Countries: 1. Publications: 3.

restrictedIPD-UNDECIDEDFeb 2026View details →
geo24/100

One deficiency, different outcomes: Succinate dehydrogenase loss in chromaffin cell vs. fibroblast models of paraganglioma

GEO Series GSE263778. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJan 2025View details →
geo24/100

Succinate Dehydrogenase Regulates Homeostasis and Metabolic Integrity of Alveolar Macrophages

GEO Series GSE288614. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenFeb 2025View details →
ClinicalTrials.gov24/100

Temozolomide (TMZ) in Advanced Succinate Dehydrogenase (SDH)-Mutant/Deficient Gastrointestinal Stromal Tumor (GIST)

ClinicalTrials.gov study NCT03556384. IPD Sharing: NO. Countries: 1. Publications: 0.

closedIPD-NOFeb 2026View details →
geo24/100

Succinate dehydrogenase deficiency-driven succinate accumulation induces drug resistance in acute myeloid leukemia via ubiquitin-cullin regulation

GEO Series GSE247623. Homo sapiens. 18 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenAug 2024View details →
geo20/100

Succinate dehydrogenase (SDH)-Complex II regulates skeletal muscle cellular respiration and fatigue but not muscle mass in genetically induced pulmonary emphysema [20 wk]

GEO Series GSE255635. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenFeb 2024View details →
geo20/100

Succinate dehydrogenase (SDH)-Complex II regulates skeletal muscle cellular respiration and fatigue but not muscle mass in genetically induced pulmonary emphysema [3 wk]

GEO Series GSE255632. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenFeb 2024View details →
geo20/100

Global transcriptional response of Saccharomyces cerevisiae following the deletion of succinate dehydrogenase

GEO Series GSE13924. Saccharomyces cerevisiae. 2 samples. Type: Expression profiling by array.

openGEO-OpenDec 2008View details →
geo16/100

Characterizing gene expression changes that accompany succinate dehydrogenase subunit B (SDHB) RNA editing in monocyte-enriched peripheral blood mononuclear cell cultures

GEO Series GSE45900. Homo sapiens. 12 samples. Type: Expression profiling by array.

openGEO-OpenApr 2013View details →
geo16/100

Itaconate links inhibition of succinate dehydrogenase with macrophage metabolic remodeling and regulation of inflammation

GEO Series GSE82043. Mus musculus. 18 samples. Type: Expression profiling by high throughput sequencing.

openGEO-OpenJul 2016View details →

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