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2 results for “sweeping net”
Raw metagenomic data from sweep net samples collected in 2016 as part of the Slikok Creek Watershed Biotic Inventory
<p>We set out to inventory vascular plants, bryophytes, lichens, birds, arthropods, and earthworms on a grid of sites in the portion of Slikok Creek watershed that is on the Kenai National Wildlife Refuge, Kenai Peninsula, Alaska. Occurrence data, images, and field data sheets from this project are available via an <a href="https://arctosdb.org/">Arctos</a> project page at <a href="http://arctos.database.museum/project/10002227">http://arctos.database.museum/project/10002227</a>.</p> <p>This dataset includes the raw FASTQ files from metagenomic processing and associated collection data. Of the 160 sweep net samples collected, 125 were selected for High Throughput Sequencing and shipped to RTL Genomics (<a href="http://rtlgenomics.com">http://rtlgenomics.com</a>) for extraction and sequencing steps. Sequencing was performed on an Illumina MiSeq platform and reads were processed using RTL Genomics’ standard methods with the mlCOIlintF/HCO2198 primer set of Leray et al. (2013), yielding a 313 bp region of the COI gene.</p> <p>Collection data are included in the file <code>ArctosData_43C6167EB1.csv</code> downloaded from Arctos. Extraction methods and sequencing methods provided by RTL Genomics are included in the files <code>Bowser 4869.pdf</code> and <code>Illumina MiSeq Two-Step Method 454 profile only.docx</code>. Primers used are provided in the file <code>Bowser_4869M.txt</code>. The archive <code>FASTQ.zip</code> contains all of the resulting FASTQ files.</p> <p>These sequence data have also been been published to GenBank's Sequence Read Archive in accessions <a href="http://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR10454582">SRR10454582</a>–<a href="http://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR10454706">SRR10454706</a> under BioProject <a href="http://www.ncbi.nlm.nih.gov/bioproject/PRJNA427721">PRJNA427721</a>.</p>
Arthropod abundance in sweep net samples of sites near Crested Butte, CO in 2017-2020
The purpose of this study was to track year-to-year variation in arthropod communities of the host plant Ligusticum porteri (Apiaceae). In each study year (2017-2020), we used sweep nets to sample the arthropods at each of twenty sites containing populations of L. porteri. Sampling took place the end of June (June 28-30th), and we standardized sampling effort across years. Two field technicians netted insects from vegetation at a consistent pace for 30 seconds each. We sorted and identified insects to Order using a dissection microscope and a project-specific taxonomic key. Non-insect arthropods were also counted but not identified to additional taxonomic levels.
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