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4 results for “target site insensitivity”
Figure 2 in Target-site insensitivity to some acaricides in a field population of Tetranychus urticae Koch (Acari: Tetranychidae) from Egypt
Figure 2. Multiple alignment of the amino acid sequences of T. urticae acetylcholinesterase (AChE) between Eg-Bernasht population and GenBank published populations. Tetranychus urticae AChE sequences were performed local as well as global alignments, using BLASTX search protein databases, using a translated nucleotide (NCBI). Three different specific primer sets (1, 2, and 3; Table 1) contained 422 amino acids of AChE gene. Dots: indicate amino acid similarity. Digital number: amino acid position on the AChE protein.
Figure 1 in Target-site insensitivity to some acaricides in a field population of Tetranychus urticae Koch (Acari: Tetranychidae) from Egypt
Figure 1. Products were imaged on a gel post electrophoresis. Tetranychus urticae acetylcholinesterase (AChE) gene fragment amplified expected sizes (639 bp, 571 bp, and 560 bp), using specific primers sets (1, 2, and 3, respectively). Tetranychus urticae voltage-gated sodium channel (VGSC) gene fragments were amplified products (226 bp, 225 bp, and 292 bp), using specific primers primer sets (4, 5, and 6, respectively). Tetranychus urticae glutamategated chloride channel (GluCl1, GluCl3) genes and chitin synthase 1 (CHS1) gene amplified products (263 bp, 251 bp, 541 bp), using primer sets (7, 8, and 9 respectively). EtBr was added to the gel before electrophoresis to a final concentration of 0.5 μg/ml, followed by separation at 100 V for 1 h. The gel was exposed to UV light and the picture was taken with a gel documentation system. Letter a: sample 1, letter b: sample 2, (-): no DNA templates. M:100 bp DNA ladder.
Dataset for: Ace and ace-like genes of invasive redlegged earth mite: Copy number variation, target-site mutations, and their associations with organophosphate insensitivity
<p class="MsoNormal">This repository contains the scripts and data required to replicate the analyses in Thia et al.'s, "Evolution of an acetylcholinesterase<em> </em>gene complex and its contribution toward organophosphate insensitivity in an invasive mite pest", submitted to <em>Pest Management Science</em>.</p> <p class="MsoNormal">In this work, Thia et al. use a combination of experimental selection and pool-seq genomic analyses to understand the genetic mechanisms underpinning organophosphate insensitivity in the redlegged earth mite, <em>Halotydeus destructor</em>. There is a special emphasis on disentangling the roles of copy number variation and target-site mutations in the acetylcholinesterase genes, <em>ace,</em> and radiated <em>ace</em>-like genes<span>.</span></p> <p class="MsoNormal">There are three major analyses: (1) an F<sub>ST</sub> genome scan to identify outlier loci between alive (insensitive) and dead (sensitive) mites; (2) an analysis of <em>ace </em>copy number variation between alive and dead mites; and (3) an analysis of candidate target-site mutations in the <em>ace</em> gene.</p>
Dataset for: Ace and ace-like genes of invasive redlegged earth mite: Copy number variation, target-site mutations, and their associations with organophosphate insensitivity
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