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34 results for “western blot”
Western blots supporting "Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase"
<p>Western blots used to generate figures 2-6 and S4 of the publication, Egbert JR, Silbern I, Uliasz TF, Lowther KM, Yee S-P, Urlaub H, and Jaffe LA. 2023. Phosphatases modified by LH signaling in ovarian follicles: testing their role in regulating the NPR2 guanylyl cyclase. <i>Biology of Reproduction</i>, ioad130. <a href="https://doi.org/10.1093/biolre/ioad130">https://doi.org/10.1093/biolre/ioad130</a></p>
Saxton & Rine 2022 Raw data for western blots
<p>Western blots in Saxton & Rine "Distinct silencer states generate epigenetic states of heterochromatin", corresponding to data in Figure S3B and Figure 2D</p>
Raw Microscopy and Western Blot Data for "Distinct silencer states generate epigenetic states of heterochromatin"
<p>Raw microscopy and western blot images for "Distinct silencer states generate epigenetic states of heterochromatin"</p>
Figure 3. SDS PAGE and Western blots with rEnSerp1 in Identification and partial characterization of a novel serpin from Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
Figure 3. SDS PAGE and Western blots with rEnSerp1, ESP and CWE. Lines 1–3, 1D gel. Lines 4–10, Western blot. M, protein standard; 1, rEnSerp1; 2, ESP; 3, CWE sample; 4, rEnSerp1 with anti-HIS antibodies; 5, rEnSerp1 without primary antibodies; 6, rEnSerp1 with anti-rEnSerp1 sera; 7, rEnSerp1 with pre-immune sera; 8, ESP with anti-rEnSerp1 sera; 9, ESP with pre-immune sera; 10, CWE with anti-rEnSerp1 sera. Arrow points to the expected recombinant EnSerp1 band. Arrowhead points to the natural form of EnSerp1 in the ESP sample. Dots indicate fragmented parts of rEnSerp1.
Fig. 2. Western blot analysis for SINV-3 capsid protein 9 d afer inoculating 6 in Solenopsis invicta virus 3: infection tests with adult honey bees (Hymenoptera: Apidae)
Fig. 2. Western blot analysis for SINV-3 capsid protein 9 d afer inoculating 6 groups of honey bees and 6 fire ant colonies. Lanes 1 and 2 show positive detection of capsid proteins in all 6 inoculated fire ant colonies (shown in 3 rows). Lanes 3 to 8 show negative tests for 18 bees (3 from each of the 6 groups inoculated with SINV-3).
mCherry fluorescence correlation with western blot band intensity
<p><strong>Overview</strong></p> <p>The results present in this dataset were obtained from recombinant <em>Chlamydomonas reinhardtii</em> expressing the fluorescent protein mCherry in different constructs formats, aiming to recombinant protein secretion. </p> <p>Image present in this dataset were obtained by following the steps described in the protocol dx.doi.org/10.17504/protocols.io.kfpctmn. </p> <p>Fluorescence measurements were obtained by growing cc1690 in 50 mL TAP media on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m<sup>2</sup>s). Then, 100 μL of each strain was transferred to a clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland). Fluorescence was measured at excitation 575/9 nm and emission 608/20 nm. Supernatant samples were obtained by spinning 1 mL sample at 15000 × <em>g</em> for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement.</p> <p><strong>Files Info</strong></p> <p>021115_20171219 Correlation_Fluore_vs_Densito_mCherry_50mL_7_days.xlsx -> data points for fluorescence measurements and pixel intensity obtained with FiJI (Fiji Is Just ImageJ). </p> <p>022615 WB SP mCHerry Lysate 3.JPG -> Raw western blot image for lysate samples</p> <p>022615 WB SP mCHerry Supernatant 3 .JPG -> Raw western blot image for supernatant samples </p> <p>20171219 Fluor_Densito_Correlation_Lys.png -> Correlation plot of fluorescence and pixel intensity for lysate samples</p> <p>WB_Lysa_7days_for_Densitom.png -> Prepared image for densitometry analysis of lysate samples</p> <p>20171219 WB_Sup_7days_for_Densitom.png -> Correlation plot of fluorescence and pixel intensity for supernatant samples</p> <p>WB_Sup_7days_for_Densitom.png -> Prepared image for densitometry analysis of supernatant samples</p> <p> </p> <p><strong>Consider citing our work. </strong></p> <p>Molino JVD, de Carvalho JCM, Mayfield SP (2018) Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS ONE 13(2): e0192433. https://doi.org/10.1371/journal. pone.0192433<br> </p> <p> </p> <p> </p>
BRAIN Journal-Suicide: Neurochemical Approaches-Figure 1. Representative bands of Western Blot showing the Protein levels of BDNF, Trk B, NGF and Trk A
<p>The molecular weights of BDNF, TrkB, NGF, TrkA and β-actin were 14 kDa, 145 kDa,<br> 13.5 kDa, 140 kDa and 46 kDa, respectively. The expression levels of BDNF, NGF, TrkB, and<br> TrkA proteins were normalized against the β-actin protein level, which was used as an internal<br> control. The results showed that the expression of BDNF, NGF, TrkB and TrkA in the hippocampus<br> decreased significantly in the suicide subjects when compared to the control subjects (P<0.05,<br> Figure 1).</p>
uncropped western blots for analysis of RPN13 ubiquitylation and NRF1 activation by protein aggregates, as well as source data for qPCR plots and flow cytometry gating and FCS files for agDD-GFP in HeLa or HEK cells
<p>This entry contains uncropped blots for Fig 4D and Fig S4C, Fig. 5B, Fig S5 and Fig S6, and the raw FCS files for Flow Cytometry data in doi.org/10.1101/2024.08.30.610524.</p>
Raw image files of uncropped western blots associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"
<p>This entry contains uncropped blots for Fig. 1C, S1C, S1D, 2I, and S3B for: https://doi.org/10.1101/2024.09.24.614704</p>
Motor usage imprints microtubule stability on the shaft - Western blots
<p>Original western blot images that have been used in Andreu-Carbo et al, Motor usage imprints microtubule stability on the shaft, bioRxiv, 10.1101/2021.04.09.439170. For details see the paper.</p>
Full western blot films related to Figure S3B
<p>This dataset contains the full Western blot films obtained to detect the presence of human dystrophin in Healthy control human induced pluripotent stem cells (hiPSCs), as they were differentiated into skeletal muscle cells, and to validate the gene editing strategy in a line of hiPSCs where the DMD gene (encoding dystrophin) was mutated by CRISPR Cas 9. Briefly, this data helped demonstrate that dystrophin is expressed only at Day 25 (D25) in Healthy hiPSCs, but not from Day 0 to Day 10. In contrast, the protein is not detected at any time point in edited cells, which validates the presence of the mutation.</p> <p>Each film includes manually written numbers "1." and "2.". For each protein target, panel 1. was obtained from teh Healthy control hiPSC line and panel 2. was obtained from the CRISPR line. The sample positionon the gels is as follows:<br> --- Positive control (3 bands) --- Negative control --- Ladder --- hiPSC D0 --- hiPSC D3 --- hiPSC D7 --- hiPSC D10 --- hiPSC D25 ---<br> The ubiquitous proteins GAPDH and alpha-tubulin are used as loading controls. Each protein was revealed on several films with various exposure times, indicated on the topright corner of the film. Each film were obtained from the same gels, after cutting the membrane in fragments according to protein target size.</p>
Western Blotting NSD3 in AML Cell Lines
<p>SGC Open Lab Notebook Project - Understanding the Role of NSD3 in Cancer </p> <p>Experiment 011 - Western blot of MOLM-13, UCSD-AML1, and HL-60 AML cell lines probing for NSD3. </p>
Shigella Congo red secretion profile unaltered Western blots
<p>These are unaltered raw Western blot images (Image Lab files) of a Congo red secretion assay testing the type three secretion profiles of <em>Shigella</em> strains expressing engineered Spa47 mutants.</p>
Dopamine signaling in the dorsomedial striatum promotes compulsive behavior -- Western Blot Supplement
<p>Compulsive behavior is a defining feature of disorders such as substance use disorders. Current evidence suggests that corticostriatal circuits control the expression of established compulsions, but little is known about the mechanisms regulating the development of compulsions. We hypothesized that dopamine, a critical modulator of striatal synaptic plasticity, could control alterations in corticostriatal circuits leading to the development of compulsions (defined here as continued reward seeking in the face of punishment). We used dual-site fiber photometry to measure dopamine axon activity in the dorsomedial striatum (DMS) and the dorsolateral striatum (DLS) as compulsions emerged. Individual variability in the speed with which compulsions emerged was predicted by DMS dopamine axon activity. Amplifying this dopamine signal accelerated animals’ transitions to compulsion, whereas inhibition delayed it. In contrast, amplifying DLS dopamine signaling had no effect on the emergence of compulsions. These results establish DMS dopamine signaling as a key controller of the development of compulsive reward seeking.</p>
Raw Western blots data to assess cold shock protein expression
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Raw western blot from the manuscript "Paradoxical neuronal hyperexcitability in a mouse model of mitochondrial pyruvate import deficiency"
<p>Uncropped western blot associated with the manuscript "Paradoxical neuronal hyperexcitability in a mouse model of mitochondrial pyruvate import deficiency"</p>
Western blots for Do, Hsu et al. 2024
<p>Raw western blots for Do, Hsu et al. "Nucleotide depletion promotes cell fate transitions by inducing DNA replication stress" Developmental Cell (2024) </p>
Raw western blots and agarose gels for "Isoginkgetin and Madrasin are poor splicing inhibitors" manuscript
<p>Raw western blots and agarose gels for "Isoginkgetin and Madrasin are poor splicing inhibitors" manuscript</p>
western blot
<p>original picture for western blot</p>
Raw images of western blot
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OpenNeuro
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