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111 results for “white blood cells”
Fluorescent Confocal Laser Scanning Microscopy of White Blood Cells, Cancer Cell Line MCF7, and Mixtures of these Cells: A Model System for Circulating Tumor Cell Biomarker Evaluation V.1
<p>This is a confocal laser scanning microscopy data set of white blood cells (leukocytes), the cancer cell line MCF7, and mixtures of these cells acquired on a Zeiss LSM 780 microscope in the University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core. Cells are fluorescently labeled for DNA with DAPI (Sigma D9542), lipids with Bodipy 495/503 (Thermo Fisher D3922), the filament protein cytokeratin (CK) with pan-cytokertain-alexa555 antibodies (Cell Signaling Technologies 3478S) and the surface membrane antigen CD45 with CD45-alexa647 antibodies (Biolegend 304020). Bodipy was excited with a continuous wave (CW) 488 nm laser, alexa555 was excited with CW 561 nm laser, and alexa647 was excited with a CW 633 nm laser. The acquiring instrument does not have a CW 405 nm source so DAPI was excited by two photon process using a Coherent Cameleon ultrafast pulsed laser tuned to 765 nm. The objective used was a Zeiss Plan-Apochromat 20x, 0.8 NA, air.</p> <p>The data consists of 4 channel 8x8 mosaic z-stacks. The Zeiss software performed stitching of the mosaics. These stitched data images are included and marked with _Stitched at the end. Those interested in performing the stitching themselves can do this with the raw data files (without the _Stitched). The jpeg images are processed from the stitched LSM images. The LSM files contain additional meta data on the experiment including power levels and acquisition settings.</p> <p>The _Stiched .lsm files will load in ImageJ (tested with V.1.49) as 4 channel 3 stack images.</p> <p>This data is a model system for evaluating the DNA/Lipids/CK/CD45 biomarker panel to identify circulating tumor cells (CTCs). The D- population of the model is the WBCs and the D+ population is the MCF7 cancer cell line. The amount of separation the biomarker panel plus analysis algorithm can produce between these populations (D+/D-) is an estimate the sensitivity and specificity of the biomarker panel plus algorithm to CTCs.</p> <p>Experiments generating the data were performed over the course of 15 days. Peripheral blood samples were collected from the Gynecological Tissue and Fluid Bank (COMIRB 07-0935 / COMIRB 05-1081) from consenting patients undergoing surgery at the University of Colorado Hospital. Blood samples were used the same day they were collected. Blood samples were collected from 3 patients with benign conditions, labeled WBBN#, and 3 patients with ovarian cancer, labeled WBCA#. We do not expect there to be any difference in the isolated white blood cells samples prepared from the cancer and benign patients. Samples were stored at room temperature until white blood cells were isolated. Mixed samples were prepared by passaging a MCF7 flask and mixing it with isolated white blood cells before fixation. A schedule showing the time duration between collection, processing and imaging is included as “experimental schedule.gif”.</p> <p>The MCF7 cancer cell line was a kind gift from Dr. Heide Ford. Genomic DNA was isolated from the MCF7 cell line after the experiment and sent for cell line authentication. The gDNA was a match to MCF7. The authentication report and data are included in this submission.</p> <p>CD45 antibodies were exhausted on day 7. New antibody was purchased and received on day 8. The day 7 images only has labels for DAPI and Bodipy. The samples prepared with the old antibodies on days 4 and 7 were relabeled and imaged with the new antibodies on days 14 and 15. This labeling was also done to confirm the pan-CK antibodies remained good since they are dim in the MCF7 cells imaged on days 12 and 13. The pan-CK on days 14 and 15 looks the same as it did on days 5 and 7 confirming the antibodies are good.</p> <p>Four of the filters containing cells were not sufficiently flat to be acquired with a 3 slice z-stack so a 5 slice z-stack was used. These files have been zipped to compress them under the 2 GB limit permitted by zenodo.org</p> <p>Further information on how these samples were prepared, processed, and analyzed can be found in our associated 2016 SPIE Photonics West BIOS conference proceeding titled, “Quantitative image cytometry measurements of lipids, DNA, CD45 and cytokeratin for circulating tumor cell identification in a model system”, http://dx.doi.org/10.1117/12.2222317.</p> <p>This work was supported by funding provided to the University of Colorado Cancer Center by the American Cancer Society and awarded as Institutional Research Grant Number 57-001-53, by funding provided by the Defense Advanced Research Projects Agency under grant number N66001-10-4035, and by funding provided by NIH/NCATS Colorado CTSI Grant Number TL1 TR001081. The University of Colorado Anschutz Medical Campus Advanced Light Microscopy Core is also supported in part by NIH/NCATS Colorado CTSI Grant Number UL1 TR001082. The funders had no role in the study design, data collection, analysis, or decision to publish.</p>
White Blood Cells with cytoplasm and surrounding red blood cells
<p>This dataset is taken from doi:10.17632/w7cvnmn4c5.1 and is used for segmentation of irregular shaped white blood cells in 2D. We also provide dataset for cytoplasm and we trained StarDist and UNET models to be applied in the VollSeg setting on this dataset.</p>
Do changes in body mass alter white blood cell profiles and immune function in Australian cane toads (Rhinella marina)?
<p><span>Variation in food resources can result in dramatic fluctuations in the body condition of animals dependent on those resources. Decreases in body mass can disrupt patterns of energy allocation and impose stress, thereby altering immune function. In this study we investigated links between changes in body mass of captive cane toads (Rhinella marina), their circulating white blood cell populations, and their performance in immune assays. Captive toads that lost weight over a 3-month period had increased levels of monocytes and heterophils and reduced levels of eosinophils. Basophil and lymphocyte levels were unrelated to changes in mass. Because individuals that lost mass had higher heterophil levels but stable lymphocyte levels, the ratio of these cell types was also higher, partially consistent with a stress response. Phagocytic ability of whole blood was higher in toads that lost mass, due to increased circulating levels of phagocytic cells. Other measures of immune performance were unrelated to mass change. These results highlight the challenges faced by invasive species as they expand their range into novel environments which may impose substantial seasonal changes in food availability that were not present in the native range. Individuals facing energy restrictions may shift their immune function towards more economical and general avenues of combating pathogens. </span></p>
Anti-CD19 White Blood Cells for Children and Young Adults With B Cell Leukemia or Lymphoma
ClinicalTrials.gov study NCT01593696. IPD Sharing: NO. Countries: 1. Publications: 9.
Activated White Blood Cells With ASCT for Newly Diagnosed Multiple Myeloma
ClinicalTrials.gov study NCT00566098. IPD Sharing: NO. Countries: 1. Publications: 1.
Melanoma Treatment With White Blood Cells That Destroy MART Expressing Tumor Cells
ClinicalTrials.gov study NCT01495572. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Do changes in body mass alter white blood cell profiles and immune function in Australian cane toads (Rhinella marina)?
Open the record for dataset details and reuse information.
Data from: Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats
Open the record for dataset details and reuse information.
Improving White Blood Cell Collection From Healthy Donors
ClinicalTrials.gov study NCT01553214. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Effects of Antibodies to White Blood Cells on Platelet Transfusion Patients
ClinicalTrials.gov study NCT00460122. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Fast-track Blood Test for Suspected Fever by Deficiency of a Kind of White Blood Cells As Main Defense Against Infection
ClinicalTrials.gov study NCT05393505. IPD Sharing: NO. Countries: 1. Publications: 22.
NK White Blood Cells and Interleukin in Children and Young Adults With Advanced Solid Tumors
ClinicalTrials.gov study NCT01875601. IPD Sharing: YES. Countries: 1. Publications: 3.
White Blood Cell Infusions to Treat HIV Infection
ClinicalTrials.gov study NCT00001647. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Dendritic Cells (White Blood Cells) Vaccination for Advanced Melanoma
ClinicalTrials.gov study NCT00683670. IPD Sharing: NO. Countries: 1. Publications: 50.
LCI Apheresis to Obtain Plasma or White Blood Cells
ClinicalTrials.gov study NCT00562601. IPD Sharing: Not stated. Countries: 1. Publications: 1.
White Blood Cell SPECT/CT and Diabetic Foot Osteomyelitis
ClinicalTrials.gov study NCT02927678. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Modified White Blood Cells That Secrete IL-2 and Express a Protein That Targets the ESO-1tumor Protein for Metastatic Cancer
ClinicalTrials.gov study NCT01457131. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Studies of White Blood Cells Derived From HHT Patients
ClinicalTrials.gov study NCT00230633. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Safety and Survival of Genetically Modified White Blood Cells in HIV-infected Twins The Gemini Study
ClinicalTrials.gov study NCT04799483. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Total-Body Irradiation Plus Stem Cell Transplantation And White Blood Cell Infusion in Treating Older Patients With Acute Myeloid Leukemia
ClinicalTrials.gov study NCT00005801. IPD Sharing: Not stated. Countries: 1. Publications: 1.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
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ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.