Conductivity and Salinity in Zebrafish Systems
Conductivity is the standard measure of dissolved ion content in recirculating aquatic systems. For zebrafish (*Danio rerio*), which are freshwater teleosts, maintaining appropriate ionic strength is essential for osmoregulation, gill health, and reproductive success.
Key ranges (µS/cm):
- <200: Ion-poor — osmotic stress, poor egg quality
- 300–500: Acceptable low range
- 500–800: Optimal for most facilities (target ~700 µS/cm)
- 800–1500: Acceptable high range
- >1500: Risk of gill irritation and impaired development
Most facilities use a blend of salts (NaCl, CaCl₂, MgSO₄, NaHCO₃) or commercial preparations (e.g., Instant Ocean at 0.3–0.5 g/L) to achieve target conductivity while also providing appropriate hardness and buffering.
Dosing Best Practices
Adjusting conductivity should be done gradually to avoid osmotic shock:
Stepwise dosing protocol:
1. Measure current conductivity with a calibrated meter
2. Calculate the total stock volume needed (this calculator does this)
3. Divide into 3–5 increments
4. Add each increment, wait 5 minutes for mixing, then re-measure
5. Stop when target is reached — do not rely solely on calculated volumes
Common pitfalls:
- Adding stock directly to the sump without mixing — creates local hot spots
- Not accounting for biofilter salt consumption (bacteria consume some ions)
- Using table salt with iodine or anti-caking agents — use aquarium-grade or reagent-grade NaCl
- Assuming conductivity is stable — it drifts due to evaporation (increases) and water changes (decreases)