Fiber Photometry Signal Calculator
Compute ΔF/F, correct photobleaching, and generate peri-event time histograms from fiber photometry recordings. Supports TDT, Doric, and generic CSV formats.
Optogenetics & PhotometryΔF/F + z-scoreClient-Side
Tool details, related tools, and citation
- Compute delta-F/F from raw fiber photometry fluorescence values
- Correct photobleaching in long recording sessions using exponential decay fitting
- Generate peri-event time histograms (PETH) aligned to behavioral events
- Compare signal magnitude across sessions using z-score normalization
- Export corrected traces and PETH summaries for downstream analysis
Don't use for
- For two-photon calcium imaging ROI extraction, use Suite2p or CaImAn for spatial segmentation
- When signal-to-noise is extremely low, consider hardware improvements first
- As a replacement for motion artifact rejection in freely-moving recordings with severe cable noise
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