Left and right paw withdrawal latency with cutoff clamping, asymmetry output, group SEM, and CSV export.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | T4 (s) | |
|---|---|---|---|---|---|---|
Cutoff: 20s. Values exceeding cutoff are clamped on calculation.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | T4 (s) | |
|---|---|---|---|---|---|---|
Cutoff: 20s. Values exceeding cutoff are clamped on calculation.
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The Hargreaves plantar test applies focused radiant heat to the plantar surface of the hindpaw through a glass floor. The endpoint is paw withdrawal latency in seconds.
The method is especially useful for unilateral inflammatory and neuropathic pain models because left and right paws can be measured separately in the same animal.
This calculator clamps each left and right paw trial at the selected cutoff. It then computes mean left latency, mean right latency, and asymmetry as right mean minus left mean.
Group charts can display left and right paw means side by side or the asymmetry endpoint. SEM is computed across animal-level means within each group.
Collect baseline latencies before injury or dosing, then keep stimulus intensity, glass floor temperature, enclosure size, and paw targeting rules constant.
Use at least 4 trials per paw when feasible, separate repeated paw stimulations by at least 5 minutes, and randomize paw order to reduce order effects.
Infrared plantar test apparatus for paw withdrawal latency measurement
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