Per-animal tail flick latency with short-cutoff clamping, group means with SEM, and CSV export for %MPE workflows.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | |
|---|---|---|---|---|---|
Cutoff: 10s. Values exceeding cutoff are clamped on calculation.
When to use
Do not use for
Cold tails can lengthen latency and warm tails can shorten it. Acclimate animals and keep room temperature stable.
The stimulus is focused on a small tail region, so repeated tests need careful spacing and rotation.
Loose restraint can add movement artifacts, while excessive restraint can increase stress. Use one restraint approach across the study.
Each animal mean is computed from non-empty trials after clamping every trial at the selected cutoff. Group summaries are computed from animal means. SEM uses sample standard deviation divided by square root of group n. All computation runs in the browser.
Last validated 2026-04-30. Calculations are designed for planning and documentation support; verify procurement decisions against manufacturer specifications or institutional SOPs.
ConductScience Tail Flick Latency Calculator (v1.0). ConductScience, Inc. 2026. Available at: https://conductscience.com/tools/tail-flick-latency-calculator
This tool performs calculations on user-entered latency data. It does not set animal welfare limits, replace IACUC-approved protocols, or determine exclusion criteria.
The tail flick test measures response latency after a thermal stimulus is applied to the tail. The radiant heat version focuses a lamp or infrared beam on a defined tail segment, while immersion protocols use heated water.
Because the endpoint is a rapid withdrawal reflex, tail flick is commonly used for spinal nociceptive transmission and opioid analgesia screening.
This calculator clamps each trial above the cutoff to the cutoff value, then computes a per-animal mean latency. Group means and SEM are calculated from the animal means.
Stimulus intensity is recorded as context only. It does not convert latency values or change the calculation.
Set the cutoff before testing and keep the same cutoff across all animals in a study. Habituate animals to restraint, rotate tail positions, and verify that baseline latencies are stable before dosing.
Document beam intensity, tail position, restraint type, ambient temperature, and the interval between repeated trials.
Define tail flick latency, stimulus rules, cutoff handling, and reporting requirements before analysis
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