Per-animal tail flick latency with short-cutoff clamping, group means with SEM, and CSV export for %MPE workflows.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | |
|---|---|---|---|---|---|
Cutoff: 10s. Values exceeding cutoff are clamped on calculation.
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The tail flick test measures response latency after a thermal stimulus is applied to the tail. The radiant heat version focuses a lamp or infrared beam on a defined tail segment, while immersion protocols use heated water.
Because the endpoint is a rapid withdrawal reflex, tail flick is commonly used for spinal nociceptive transmission and opioid analgesia screening.
This calculator clamps each trial above the cutoff to the cutoff value, then computes a per-animal mean latency. Group means and SEM are calculated from the animal means.
Stimulus intensity is recorded as context only. It does not convert latency values or change the calculation.
Set the cutoff before testing and keep the same cutoff across all animals in a study. Habituate animals to restraint, rotate tail positions, and verify that baseline latencies are stable before dosing.
Document beam intensity, tail position, restraint type, ambient temperature, and the interval between repeated trials.
Radiant heat tail flick apparatus for spinal reflex pain testing
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