Left and right paw withdrawal latency with cutoff clamping, asymmetry output, group SEM, and CSV export.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | T4 (s) | |
|---|---|---|---|---|---|---|
Cutoff: 20s. Values exceeding cutoff are clamped on calculation.
| Animal ID | Group | T1 (s) | T2 (s) | T3 (s) | T4 (s) | |
|---|---|---|---|---|---|---|
Cutoff: 20s. Values exceeding cutoff are clamped on calculation.
When to use
Do not use for
Testing every animal in the same left-right order can add order effects. Rotate or randomize paw order and keep the inter-trial interval consistent.
Animals need time to acclimate on the glass floor. Temperature drift can shift withdrawal latency independently of injury or drug effect.
The calculator defines asymmetry as right minus left. If your injured paw is left, interpret the sign accordingly or relabel your data before export.
Left and right paw trials are clamped at the selected cutoff before averaging. Asymmetry is computed as mean right latency minus mean left latency. Group summaries are computed from animal-level means and asymmetry values. SEM uses sample standard deviation divided by square root of group n. All computation runs in the browser.
Last validated 2026-04-30. Calculations are designed for planning and documentation support; verify procurement decisions against manufacturer specifications or institutional SOPs.
ConductScience Hargreaves Latency Calculator (v1.0). ConductScience, Inc. 2026. Available at: https://conductscience.com/tools/hargreaves-latency-calculator
This tool performs calculations on user-entered latency data. It does not set animal welfare limits, replace IACUC-approved protocols, or determine exclusion criteria.
The Hargreaves plantar test applies focused radiant heat to the plantar surface of the hindpaw through a glass floor. The endpoint is paw withdrawal latency in seconds.
The method is especially useful for unilateral inflammatory and neuropathic pain models because left and right paws can be measured separately in the same animal.
This calculator clamps each left and right paw trial at the selected cutoff. It then computes mean left latency, mean right latency, and asymmetry as right mean minus left mean.
Group charts can display left and right paw means side by side or the asymmetry endpoint. SEM is computed across animal-level means within each group.
Collect baseline latencies before injury or dosing, then keep stimulus intensity, glass floor temperature, enclosure size, and paw targeting rules constant.
Use at least 4 trials per paw when feasible, separate repeated paw stimulations by at least 5 minutes, and randomize paw order to reduce order effects.
Infrared plantar test apparatus for paw withdrawal latency measurement
Browse EquipmentNormalize baseline and post-drug paw withdrawal latencies to %MPE
Browse EquipmentAnalyze heated surface response latency with cutoff handling
Browse EquipmentAnalyze radiant heat tail flick latency with short-cutoff handling
Browse