Identification of ovarian granulosa cells and effect examination

<p>Figure S1. Identification of ovarian granulosa cells.<br> Figure S2. The mensuration of over-expression effect. (A: The relative mRNA level of over-expression MT1, MT2, Cry1, and Cry2 at 24h by Q-PCR; B: Western blotting (WB) results of MT1, MT2, CRY1, and CRY2 protein levels, respectively. Data are presented as the mean &plusmn; standard error of the mean ( SEM) of triplicate experiments. ** P &lt; 0.01, * P &lt; 0.05)<br> Figure S3. The mensuration of silencing effect. (A-D: Q-PCR assay of the MT1, MT2, Cry1, and Cry2 mRNA levels in ovarian granular cells after transfection of siRNAs against MT1, MT2, Cry1, and Cry2, respectively. E: Western blotting (WB) results of MT1, MT2, CRY1, and CRY2 protein levels in ovarian granular cells after transfection of siRNAs against MT1, MT2, Cry1, and Cry2, respectively. Data are presented as the mean &plusmn; standard error of the mean ( SEM) of triplicate experiments. ** P &lt; 0.01, * P &lt; 0.05)</p>