Fig. 1 in Isolation of an archaeon at the prokaryote eukaryote interface

Fig. 1 | Growth curves and photomicrographs of the cultured Lokiarchaeota strain MK-D1. a, Growth curves of MK-D1 in anaerobic medium supplemented with casamino acids (CA) alone; casamino acids with 20 amino acids (AAs) and powdered milk (PM); or peptone with powdered milk. Results are also shown for cultures fed with 10- and 100-fold dilution of casamino acids,20 amino acids and powdered milk.b, c, Fluorescence images of cells from enrichment cultures after 8 (b) and 11 (c) transfers stained with DAPI (violet) and hybridized with nucleotide probes that target MK-D1 (green) and Bacteria (red). Pie charts show the relative abundance of microbial populations based on SSU rRNA gene-tag sequencing (iTAG) analysis.d, A fluorescence image of cells from enrichment cultures after 11 transfers hybridized with nucleotide probes that target MK-D1 (green) and Methanogenium (red).The FISH experiments were performed three times with similar results.e, SEM image of a highly purified co-culture of MK-D1 and Methanogenium. White arrows indicate Methanogenium cells.We observed four different co-cultures with Methanogenium. Representative of n = 40 recorded images.The detailed iTAG-based community compositions of cultures corresponding to each of the images are shown in Supplementary Table 1. Scale bars,10 µm (b, c) and 5 µm (d, e).