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Stem trichome mRNA-Seq dataset from the 20 accessions (part 5)

<p><strong>RNA isolation and sequencing</strong>&nbsp;</p> <p>Total RNA were isolated using the RNeasy Plant Mini Kit (QIAGEN) from 50 to 150mg of stem trichome material. RNA concentration and quality was assessed using the Agilent 2100 Bioanalyzer instrument (Agilent).&nbsp;&nbsp;mRNA sequencing was done using the Proton Ion system using PI chips and yielded between 25 and 33 million reads per sample.</p> <p><strong>Table of genotypes used</strong></p> <p>&nbsp;</p> <pre><code class="language-markdown">| accession | species | accession nr | synonym | origin | |------------|--------------------------------|--------------|---------|-------------| | LA2172 | S. arcanum | TR0009 | - | Peru | | LA1401* | S. cheesmaniae f. minor | EA00652 | - | Ecuador | | LA1840 | S. chmielewskii | - | - | unknown | | LA2695 | S. chmielewskii | EA00759 | - | Peru | | LA0407 | S. habrochaites f. glabratum | EA00558 | - | Ecuador | | LA1777 | S. habrochaites f. hirsutum | EA00703 | - | Peru | | PI134418 | S. habrochaites f. glabratum | TR00015 | LYC38 | unknown | | LYC4 | S. habrochaites f. hirsutum | TR00017 | - | unknown | | LA1718 | S. habrochaites f. glabratum | EA00699 | LYC4934 | Peru | | PI127826 | S. habrochaites f. hirsutum | - | - | Peru | | LA1364 | S. huaylasense | TR00030 | - | Peru | | Moneymaker | S. lycopersicum | - | C32 | Netherlands | | LA4024 | S. lycopersicum | TA209 | - | unknown | | LA2133 | S. neorickii/L. parviflorum | EA00729 | - | Peru | | LA0735 | S. neorickii | TR00025 | LYC140 | unknown | | LA0716 | S. pennellii | EA00585 | - | Peru | | LA1278 | S. peruvianum/pimpinellifolium | TR00005 | - | unknown | | LA1954 | S. peruvianum | EA00713 | - | Peru | | LA1578 | S. pimpinellifolium | EA00674 | - | Peru |</code></pre> <p>&nbsp;</p>

ShareScore

16/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
0
Reuse readiness
0
Engagement
4

Topics

Dataset details

Repository
zenodo
Published
February 15, 2016
License
Not reported
DOI
10.5281/zenodo.3611143

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