Homogeneous multifocal excitation for high-throughput super-resolution imaging - Expanded centriole particles
<p>Datasets containing the segmented expanded centriole particles. The prefix Hs is used to denote particles acquired in synchronized RPE-1 human cells. Otherwise particles were collected from expanded isolated centrioles from <em>Chlamydomoanas reinhardtii</em>. Resized datasets have uniform voxel size of 14x14x14 nm3 after expansion (56x56x56 nm3 before expansion). Non-resized datasets have 14x14x30 pixel size (56x56x120 nm3 before expansion). All files should be mirrored horizontally/vertically to account for the chirality inversion due to the imaging process</p> <p>The channels in different datasets are:</p> <ul> <li>Chlamy acetylated sample: <ul> <li>C1: acetylated tubulin-Alexa488</li> <li>C2: aTubulin-Alexa568</li> </ul> </li> <li>Chlamy MonoE sample <ul> <li>C1: aTubulin-Alexa488</li> <li>C2: GT335-Alexa568</li> </ul> </li> <li>Chlamy PolyE sample <ul> <li>C1: PolyE-Alexa488</li> <li>C2: aTubulin-Alexa568</li> </ul> </li> <li>Hs sample: <ul> <li>C1: PolyE-Alexa488</li> <li>C2: acetylated tubulin-Alexa586</li> </ul> </li> </ul>
ShareScore
40/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 4