FIGURE 4 in Uncovering a hidden diversity: a new species of freshwater shrimp Macrobrachium (Decapoda: Caridea: Palaemonidae) from Neotropical region (Brazil) revealed by morphological review and mitochondrial genes analyses

FIGURE 4. Left: Phylogenetic tree obtained from maximum likelihood analysis (ML) of 16S sequences of Macrobrachium rasiliense (Heller, 1862) and Macrobrachium veredensis sp. nov. Highlighted numbers on nodes represent the posterior probabilities obtained by Bayesian Inference analysis of 16S sequences and numbers without asterisk are bootstrap obtained by ML. Right: Haplotype network based on Median-Joining analysis of COI sequences, indicating the distribution of each haplotype found in both species. The size of the circles is proportional to the haplotype frequency. The haplotype identification is below each circle. Different colors represent populations from different states of Brazil: Pará (PA), Tocantins (TO), Mato Grosso (MT), São Paulo (SP), Minas Gerais (MG). The length of the lines between circles is proportional to mutational steps among the haplotypes, except between haplotypes from Minas Gerais (MG) (Macrobrachium veredensis sp. nov.) and the others (Macrobrachium brasiliense), where three small traces represent a lot of the mutational step. Yellow star indicates median vector.