SUPPLEMENTAL FIGURES S1-S2

<p>Fig. S1. Generation of Muc5b-EGFP reporter mouse. A. Schematic view of the<br> generation of the reporter mouse into the exon 3 of the Muc5b-/- gene. B. Next page.<br> Sequence of the final modified gene with the inserted EGFP</p> <p>Fig. S2. Validation of the MUC5B antibody staining. A. Carnoy-fixed paraffin-embedded lung<br> section of elastase-exposed WT mouse intrapulmonary airway stained with MUC5B<br> antiserum at 1:5,000 dilution. B. Carnoy-fixed paraffin-embedded lung section of elastaseexposed<br> Muc5b-/- mouse intrapulmonary airway stained with MUC5B antiserum at 1:5,000<br> dilution. Nuclei counter stained with Hoechst.</p>