Dr. Manuele Gori

<p><strong>Supplementary Figure 1. </strong>Immunohistochemical analysis of the TJ and AJ proteins with unchanged expression.</p> <p>LPS-treated vs. control monolayers did not show any differential expression or discontinuous distribution of tight junction protein 1 (TJP1, <strong>a</strong>), E-cadherin (CDH1, <strong>b</strong>) and b-catenin (CTNNB1, <strong>c</strong>) as well as in the ethanol-treated vs. control monolayers for TJP1 (<strong>d</strong>) as shown in the representative immunofluorescence micrographs. The far right histograms show the relative expression of the fluorescence intensity (FITC) on the cell surface of Caco-2 monolayers treated with LPS vs. controls (<strong>a</strong>-<strong>c</strong>) and ethanol vs. controls (<strong>d</strong>) by perimeter analysis. Data are reported as mean &plusmn; SEM; n = 3 independent experiments. Statistical analysis was performed using unpaired t-test. Scale bars: 25 &mu;m. Legend: Ctrl (Control); LPS (Lipopolysaccharide); EtOH (Ethanol).</p> <p>&nbsp;</p>