Human nasal epithelial organoids for therapeutic development in cystic fibrosis

<p>Table S1: Individual subject demographics and available clinical data for selected participants contributing functional data. Figure S1: Example measurement of &micro;OCT ciliary beat frequencies, Figure S2: Organoid CFTR (green) co-localization with apical ZO-1 (red). (A) To illustrate the location of the ZO-1 staining within the organoid, an alpha-blended 3-D volume reconstruction was created with the fluorescence intensity increased to reveal the organoid&rsquo;s shape and orient the reader to the location of the 2D co-localization image below. (B) Magnified view of the yellow frame in (A) to better visualize the CFTR and ZO-1 overlay, Video S1: Brightfield video of luminal movement of mucus, Video S2: &micro;OCT video of luminal movement of mucus, Video S3: Brightfield video of luminal cilia beating, Video S4: &micro;OCT video of cilia beating, Video S5: FIS assay of non-CF organoids, Video S6: FIS assay of F508del/P67L organoids. Video S7: FIS assay of F508del/F508del organoids.</p>