kDPRA trainig Video

<pre>The kinetic direct peptide reactivity assay (kDPRA) is an extension of the DPRA, which is already internationally recognized as Test Guideline No. 442C of the OECD. While the DPRA only provides information about a skin sensitizing potential, the kDPRA can also measure the potency, i.e. the effectiveness of skin sensitization. For this purpose, the reaction kinetics of peptide depletion are measured in the kDPRA. A distinction can be made between strong and weak skin-sensitizing effects, which are then classified according to CLP / GHS sub-categories 1A and 1B. In the kDPRA, the reaction kinetics of the reaction of the test substance with a synthetic cysteine-containing heptapeptide is evaluated. For this purpose, it is measured how much peptide reacts with different test substance concentrations after different reaction times. After the respective reaction time, the reaction is stopped by adding the highly reactive and non-fluorescent monobromobimane. This binds to peptides that have not reacted with the test substance, creating a fluorescent product that is quantified by fluorescence measurement. The peptide depletion, the amount of peptide that had reacted with the test substance, results from the difference between the peptide starting material and the fluorescent product. The reaction kinetic constant, k, is calculated by interpolation from the test substance concentration, reaction time and depletion matrix.</pre> <p>Wareing, Britta, et al. &quot;The kinetic Direct Peptide Reactivity Assay (kDPRA): Intra-and inter-laboratory reproducibility in a seven-laboratory ring trial.&quot; <em>ALTEX-Alternatives to animal experimentation</em> (2020).&nbsp; <a href="https://doi.org/10.14573/altex.2004291">https://doi.org/10.14573/altex.2004291 </a></p>