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Development and Optimization of an IQF USP5 Activity Assay

<p>Development and optimization of&nbsp;a fluorescence based deubiquitinase (DUB) activity assay for USP5 with internally quenched fluorophore pair (IQF) substrates: di-ubiquitin lysine linked K48 and K63 (Ub2K48, Ub2K63). The inhibitory&nbsp;capability of USP5 zinc-finger ubiquitin binding domain (ZnF-UBD) ligands was investigated.&nbsp;</p>

ShareScore

36/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
16
Reuse readiness
8
Engagement
0

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