Dataset related to article "Immune infiltrating cells in duodenal cancers"

<p>This record contains data related to article&nbsp;&quot;Immune infiltrating cells in duodenal cancers&quot;</p> <p>&nbsp;</p> <p>Abstract.</p> <p>&nbsp;Background: Duodenal adenocarcinoma (DA) is a rare yet aggressive malignancy, with increasing incidence in the</p> <p>last decades. Its low frequency has hampered a thorough understanding of the pathogenesis of the disease and of</p> <p>its biology, limiting the identification of tailored therapeutic options. A large body of evidence has clearly shown the</p> <p>clinical relevance of immune cells in solid tumors, correlating immune features with post-surgical prognosis. The aim</p> <p>of this study was to analyze the immune contexture in a cohort of duodenal adenocarcinomas surgically resected at</p> <p>our Institution and define its correlation with clinical variables.</p> <p>&nbsp;Methods: Tissue slides from paraffin-embedded tumor specimens of 15 consecutive DA and 3 adenomas that</p> <p>underwent a pancreaticoduodenectomy in our center between 2010 to 2018 were immunohistochemically stained.</p> <p>The density (percentage of immune reactive area, IRA%) of immune markers CD45RO, CD8, CD20, IL-17, PD-1, CD68</p> <p>was quantified by computer-assisted image analysis. Demographic, clinical, histopathological data were collected.</p> <p>&nbsp;Results: In our population, median IRA % (IQR) of immune subsets was respectively CD45RO-TILs 2.19 (2.14), CD8-TIL</p> <p>0.42 (0.81), CD20-TILs 0.22 (0.51), CD20-TLT 2.84 (4.64), CD68-TAM 2.19 (1.56), IL17+</p> <p>cells 0.39 (0.39), PD1-TILs 0.19 (0.41).</p> <p>The median follow-up was 47.5 (22.4&ndash;63.3) months. At statistical analysis, the density of CD8-TILs inversely correlated</p> <p>with lymph node ratio (p = 0.013), number of metastatic lymph nodes (p = 0.019), and was lower in N+ adenocarcinomas</p> <p>compared to N0 (1.07 vs 0.29; p = 0.093), albeit not significantly. Stratifying patients for the N status, the density of</p> <p>CD8-TILs decreased with the increasing of the N stage (p = 0.065) and was lower in patients who experienced recurrence</p> <p>and died for the disease (0.276 vs 0.641; p = 0.044). Notably, also CD68-TAM distribution was different in patients</p> <p>who had recurrence versus patients who did not (1.028 vs 2.276; p = 0.036).</p> <p>&nbsp;Conclusions: Immune cells showed variable expression in correlation with common prognostic factors, suggesting</p> <p>T cell infiltration may play a protective role towards lymphatic spread of disease and nodal metastatization. Furthermore,</p> <p>T cell density and macrophage infiltration were associated to a lower risk of recurrence and disease related</p> <p>death. A multicentric approach may be indicated to allow analysis of larger cohorts of patients, potentially increasing</p> <p>the power of our observations.</p>